|
来源
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用途
|
交叉反应性
|
分子量
|
|
Rabbit
|
IHC
|
M
|
-
|
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
-
|
-
|
-
|
1:200
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-
|
-
|
-
|
-
|
抗体详细信息如下:
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About this Antibody
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Name
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OOEP Rabbit Polyclonal Antibody
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Category
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Polyclonal antibody(pAb); Primary antibody
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|
Isotype
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IgG
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Purification
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Peptide affinity purified
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About the Immunogen
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|
Immunogen
|
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to mouse Ooep.
|
|
Gene ID
|
441161
|
|
SwissProt
|
A6NGQ2
|
|
Synonyms
|
KHDC2; FLOPED; HOEP19; C6orf156
|
|
Category
|
Chromatin Regulation / Nuclear Function
|
|
Background
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OOEP (oocyte-expressed protein homolog), also known as KHDC2 (KH homology domain-containing protein 2) or OEP19 (oocyte- and embryo-specific protein 19), is a 149 amino acid cytoplasmic protein that belongs to the KHDC1 family and contains one KH domain. As a member of the subcortical maternal complex (SCMC), OOEP is necessary for zygotes to progress beyond the first embryonic cell divisions. In addition to OOEP, the SCMC includes NALP5 and TLE6. The gene that encodes OOEP consists of approximately 26,579 bases and maps to human chromosome 6q13. With 170 million base pairs, chromosome 6 comprises nearly 6% of the human genome. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Additionally, Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map to chromosome 6.
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包装清单：
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产品编号
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产品名称
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包装
|
|
AF0252
|
OOEP Rabbit Polyclonal Antibody
|
50μl
|
|
AZ050
|
Western一抗稀释液
|
50ml
|
|
—
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说明书
|
1份
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保存条件：
OOEP Rabbit Polyclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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