|
来源
|
用途
|
交叉反应性
|
分子量
|
|
Mouse
|
WB, FC, IHC, ICC, IF
|
H, M, R
|
62KDa
|
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:500-1:1000
|
-
|
1:200
|
1:200
|
1:200
|
1:50-1:100
|
-
|
-
|
抗体详细信息如下:
|
About this Antibody
|
|
Name
|
SQSTM1/p62 Mouse Monoclonal Antibody
|
|
Category
|
Monoclonal antibody(mAb); Primary antibody
|
|
Isotype
|
IgG1
|
|
Purification
|
ProA affinity purified
|
|
About the Immunogen
|
|
Immunogen
|
This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to SQSTM1.
|
|
Gene ID
|
8878
|
|
SwissProt
|
Q13501
|
|
Synonyms
|
OSIL antibody; Oxidative stress induced like antibody p60 antibody p62 antibody p62B antibody Paget disease of bone 3 antibody PDB 3 antibody PDB3 antibody
|
|
Category
|
Autophagy
|
|
Background
|
Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress and autophagy. It was first identified as a protein that binds to the SH2 domain of p56Lck and independently found to interact with PKCζ. SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome. Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway. It may play a role in titin/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. P62 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.
|
包装清单：
|
产品编号
|
产品名称
|
包装
|
|
AF0279
|
SQSTM1/p62 Mouse Monoclonal Antibody
|
50μl
|
|
AZ050
|
Western一抗稀释液
|
50ml
|
|
—
|
说明书
|
1份
|
保存条件：
SQSTM1/p62 Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
微信在线咨询
微信在线咨询
