碧云天生物技术-Phospho-p38 MAPK抗体(小鼠单抗)(AM063)

试剂> 抗体> MAPK与PI3K/Akt通路> p38 MAPK Signaling

Phospho-p38 MAPK抗体(小鼠单抗)

一键复制产品信息

产品编号: AM063

产品包装:>20次

选择包装

>20次

数

量

说明书下载

价格: ¥ 612.00

加入购物车

				产品简介
			
				使用说明
			
				产品文件
			
				相关产品
			
				相关论文
			
				产品问答

产品编号	产品名称	产品包装	产品价格
AM063	Phospho-p38 MAPK抗体(小鼠单抗)	>20次	612.00元

来源	用途	交叉反应性	抗体类型	p38 MAPK分子量
Mouse	WB, IP, F	H, M, R,	IgG1	43kD

      WB, Western blot; IP, Immunoprecipitation; F, Flow cytometry.
      H, human; M, mouse; R, rat.
      本Phospho-p38 MAPK(Thr180/Tyr182)抗体(Phospho-p38 MAPK(Thr180/Tyr182)antibody)为进口分装，用经过适当修饰的含有磷酸化Thr180/Tyr182的一段human p38 MAPK多肽为抗原制备而成的抗Phospho-p38 MAPK(Thr180/Tyr182)小鼠单克隆抗体。克隆号为28B10。
      本Phospho-p38 MAPK抗体不识别磷酸化或非磷酸化的p44/42 MAPK，也不识别磷酸化或非磷酸化的SAPK/JNK。
      本抗体检测Thr180和Tyr182同时被磷酸化的p38 MAPK，常用于检测p38 MAPK的激活。
      p38 MAP kinase(MAPK)也称RK或CSBP，是酵母中HOG kinase在哺乳动物中的同源蛋白，在细胞因子和应激(stress)诱导的信号转导过程中起重要作用。p38 MAPK可以被炎性细胞因子、LPS、紫外线、生长因子以及一些stress所激活。MKK3和SEK可以通过磷酸化p38 MAPK的Thr180和Tyr182，从而激活p38 MAPK。激活的p38 MAPK可以磷酸化激活MAPKAP kinase等，并最终激活ATF-2、Max和MEF2等转录因子。注：上述氨基酸残基的位置为相对应于human p38 MAP kinase的氨基酸位置。
      配套提供了Western一抗稀释液，可以用于Western检测时的一抗稀释。
      建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB	IP	F
1:1000	1:25	1:500

本抗体如果用于常规的Western检测，至少可以检测20次。
包装清单：

产品编号	产品名称	包装
AM063-1	Phospho-p38 MAPK抗体(小鼠单抗)	20μl
AM063-2	Western一抗稀释液	20ml
－	说明书	1份

保存条件：
      Phospho-p38 MAPK抗体-20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
      对于本抗体，Western检测时一抗要4℃缓慢摇动过夜，如果仅短时间与一抗孵育检测效果较差。
      在Western实验后，请注意回收稀释的抗体。回收的抗体在进行Western实验时至少可以重复使用10次。稀释后的抗体，包括已经使用过的稀释抗体，4℃保存。
      回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
      为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1.Western检测：
A.按照1:1000用碧云天提供的Western一抗稀释液稀释抗体。
B.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
C.回收稀释的一抗，4℃保存以备下次继续使用。
D.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页： http://www.beyotime.com/support/immunol-staining.htm
3.其它实验操作请自行参考适当的protocol进行。

物质安全数据单

输入右侧验证码点击下载：

下载

质检证书

输入批号搜寻COA

搜索

相关产品请点击如下按钮：

p38 MAPK Signaling

使用本产品的文献：

1. Li J, Qian W, Xu Y, Chen G, Wang G, Nie S, Shen B, Zhao Z, Liu C, Chen K.

Activation of RAW 264.7 cells by a polysaccharide isolated from Antarctic bacterium Pseudoaltermonas sp. S-5.

CARBOHYD POLYM. 2015 Oct 5;130:97-103. (IF 7.182)

2. Zhang S, Tu Y, Sun YM, Li Y, Wang RM, Cao Y, Li L, Zhang LC, Wang ZB

Swiprosin-1 deficiency impairs macrophage immune response of septic mice.

JCI Insight. 2018 Feb 8;3(3). pii: 95396. (IF 6.205)

3. Zhang L, Liu X, Ma X, Liu Y, Che S, Cui J, An X, Cao B, Song Y

Testin was regulated by circRNA3175-miR182 and inhibited endometrial epithelial cell apoptosis in pre-receptive endometrium of dairy goats.

J Cell Physiol. 2018 Oct;233(10):6965-6974. (IF 5.546)

4. Xu Y, Li J, Ju J, Shen B, Chen G, Qian W, Zhu L, Lu J, Liu C, Qin G, Wang G, Chen K.

Exopolysaccharide from Trichoderma pseudokoningii promotes maturation of murine dendritic cells.

Int J Biol Macromol. 2016 Nov;92:1155-61. (IF 5.162)

5. Mimi Mu, Peiyu Gao, Qian Yang, Jing He, Fengjiao Wu, Xue Han, Shujun Guo, Zhongqing Qian, Chuanwang Song

Alveolar Epithelial Cells Promote IGF-1 Production by Alveolar Macrophages Through TGF-β to Suppress Endogenous Inflammatory Signals

Front Immunol. 2020 Jul 21;11:1585. doi: 10.3389/fimmu.2020.01585. (IF 5.085)

6. Chen S, Yin DK, Yao WB, Wang YD, Zhang YR, Gao XD.

Macrophage receptors of polysaccharide isolated from a marine filamentous fungus Phoma herbarum YS4108.

Acta Pharmacol Sin. 2009 Jul;30(7):1008-14. (IF 5.064)

7. Li H, Zhang L, Huang Q.

Differential expression of mitogen-activated protein kinase signaling pathway in the hippocampus of rats exposed to chronic unpredictable stress.

Behav Brain Res. 2009 Dec 14;205(1):32-7. (IF 2.977)

8. Khan M, Ding C, Rasul A, Yi F, Li T, Gao H, Gao R, Zhong L, Zhang K, Fang X, Ma T.

Isoalantolactone induces reactive oxygen species mediated apoptosis in pancreatic carcinoma PANC-1 cells.

Int J Biol Sci. 2012;8(4):533-47. (IF 4.858)

9. Shan R, Chen L, Li X, Wu H, Liang Q, Tang X.

Hypoxia promotes rabbit pulmonary artery smooth muscle cells proliferation through a 15-LOX-2product 15(S)-hydroxyeicosatetraenoic acid.

PROSTAG LEUKOTR ESS. 2012 Jan-Feb;86(1-2):85-90. (IF 2.932)

10. Xu S, Wen H, Jiang H.

Urotensin II promotes the proliferation of endothelial progenitor cells through p38 and p44/42 MAPK activation.

Mol Med Rep. 2012 Jul;6(1):197-200. (IF 2.1)

11. Zhou BR, Huang QH, Xu Y, Wu D, Yin ZQ, Luo D.

Dihydrotestosterone induces SREBP-1 expression and lipogenesis through the phosphoinositide 3-kinase/Aktpathway in HaCaT cells.

Lipids Health Dis. 2012 Nov 15;11:156. (IF 2.906)

12. Mao J, Liu J, Pang X, Li M, Song J, Han C, Wu D, Wang S.

Nicotine induces the expression of C-reactive protein via MAPK-dependent signal pathway in U937 macrophages.

Mol Cells. 2012 Nov;34(5):457-61. (IF 4.081)

13. Liu Y, Su WW, Wang S, Li PB.

Naringin inhibits chemokine production in an LPS-induced RAW 264.7 macrophage cell line.

Mol Med Rep. 2012 Dec;6(6):1343-50. (IF 2.1)

14. Song L, Zhu Y, Jin M, Zang B.

Hydroxysafflor yellow a inhibits lipopolysaccharide-induced inflammatory signal transduction in human alveolarepithelial A549 cells.

Fitoterapia. 2013 Jan;84:107-14. (IF 2.527)

15. Qiu M, Chen Y, Cheng L, Chu Y, Song HY, Wu ZW.

Pyrrolidine dithiocarbamate inhibits herpes simplex virus type 1 and 2 replication and its activity may be mediated through dysregulation of ubiquitin-proteasome system.

J Virol. 2013 Aug;87(15):8675-86. (IF 4.501)

16. Jin M, Sun CY, Pei CQ, Wang L, Zhang PC.

Effect of Safflor Yellow Injection on inhibiting lipopolysaccharide-induced pulmonary inflammatory injury in mice.

Chin J Integr Med. 2013 Nov;19(11):836-43. (IF 1.545)

17. Pang X, Liu J, Zhao J, Mao J, Zhang X, Feng L, Han C, Li M, Wang S, Wu D.

Homocysteine induces the expression of C-reactive protein via NMDAr-ROS-MAPK-NF-κB signal pathway in rat vascular smooth muscle cells.

Atherosclerosis. 2014 Sep;236(1):73-81. (IF 3.919)

18. Jiang S, Zhao L, Lu Y, Wang M, Chen Y, Tao D, Liu Y, Sun H, Zhang S, Ma Y.

Piwil2 inhibits Keratin 8 degradation through promoting p38-induced phosphorylation to resist Fas-mediated apoptosis.

Mol Cell Biol. 2014 Nov;34(21):3928-38. (IF 3.611)

19. Ji H, Lu X, Wang C, Zong H, Fang H, Sun J, Zhuge J, Zhuge B.

Identification of a Novel HOG1 Homologue from an Industrial Glycerol Producer Candida glycerinogenes.

Curr Microbiol. 2014 Dec;69(6):909-14. (IF 1.746)

20. Zhang M, Wang G, Tao Y, Zhang H.

The proinflammatory effect and molecular mechanism of IL- 17 in the intestinal epithelial cell line HT-29.

J BUON. 2015 Jan-Feb;20(1):120-7. (IF 1.695)

21. Li L, Tang J, Sun Y, Wu J, Yu P, Wang G.

Upregulation of HO-1 Attenuates LPS-Stimulated Proinflammatory Responses Through Downregulation of P38 Signaling Pathways in Rat Ovary.

Inflammation. 2015 Jun;38(3):1085-92. (IF 3.212)

22. Huang EW, Liu CZ, Liang SJ, Zhang Z, Lv XF, Liu J, Zhou JG, Tang YB, Guan YY.

Endophilin-A2-mediated increase in scavenger receptor expression contributes to macrophage-derivedfoam cell formation.

Atherosclerosis. 2016 Nov;254:133-41. (IF 3.919)

23. Li Y, He S, Tang J, Ding N, Chu X, Cheng L, Ding X, Liang T, Feng S, Rahman SU, Wang X, Wu J.

Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κB/MAPK Signaling Pathway.

EVID-BASED COMPL ALT. 2017;2017:8248142. (IF 1.813)

24. Pan J, Zhang L, Xu S, Cheng X, Yu H, Bao J, Lu R

Induction of Apoptosis in Human Papillary-Thyroid-Carcinoma BCPAP Cells by Diallyl Trisulfide through Activation of the MAPK Signaling Pathway.

J AGR FOOD CHEM. 2018 Jun 13;66(23):5871-5878. (IF 4.192)

25. Chandra Roy A, Wang Y, Zhang H, Roy S, Dai H, Chang G, Shen X

Sodium Butyrate Mitigates iE-DAP Induced Inflammation Caused by High-Concentrate Feeding in Liver of Dairy Goats.

J AGR FOOD CHEM. 2018 Aug 29;66(34):8999-9009. (IF 4.192)

26. Li Y, Duan F, Zhou X, Pan H, Li R

Differential responses of GC‑1 spermatogonia cells to high and low doses of bisphenol A.

Mol Med Rep. 2018 Sep;18(3):3034-3040. (IF 2.1)

27. Wang H, Zhang H, Cao F, Lu J, Tang J, Li H, Zhang Y, Feng B, Tang Z

Protection of insulin‑like growth factor 1 on experimental peripheral neuropathy in diabetic mice.

Mol Med Rep. 2018 Nov;18(5):4577-4586. (IF 2.1)

28. Gao Z, Zhao GS, Lv Y, Peng D, Tang X, Song H, Guo QN

Anoikis‑resistant human osteosarcoma cells display significant angiogenesis by activating the Src kinase‑mediated MAPK pathway.

Oncol Rep. 2019 Jan 41(1):235-245. (IF 3.417)

29. Chang G, Ma N, Zhang H, Wang Y, Huang J, Liu J, Dai H, Shen X

Sodium Butyrate Modulates Mucosal Inflammation Injury Mediated by GPR41/43 in the Cecum of Goats Fed a High Concentration Diet.

Front Physiol. 2019 Aug 30 10:1130. (IF 3.367)

30. Huang C, Wang XL, Qi FF, Pang ZL

Berberine inhibits epithelial-mesenchymal transition and promotes apoptosis of tumour-associated fibroblast-induced colonic epithelial cells through regulation of TGF-β signalling.

J Cell Commun Signal. 2019 Aug 9 (IF 3.788)

31. Wang K, Zou P, Zhu X, Zhang T

Ziyuglycoside II suppresses the aggressive phenotype of triple negative breast cancer cells through regulating Src/EGFR-dependent ITGB4/FAK signaling.

Toxicol In Vitro. 2019 Dec 61:104653. (IF 2.959)

32. Shi S, Zhang S, Zhang H, Jin Q, Wu D

Silencing circANKRD36 protects H9c2 cells against lipopolysaccharide-induced injury via up-regulating miR-138.

Exp Mol Pathol. 2019 Dec 111:104300. (IF 2.28)

33. Peng L, Hu C, Zhang C, Lu Y, Man S, Ma L

Anti-cancer activity of Conyza blinii saponin against cervical carcinoma through MAPK/TGF-β/Nrf2 signaling pathways.

J Ethnopharmacol. 2019 Dec 28 251:112503. (IF 3.69)

34. Lv H, Zhang S, Hao X

Swainsonine protects H9c2 cells against lipopolysaccharide-induced apoptosis and inflammatory injury via down-regulating miR-429.

Cell Cycle. 2020 Jan 19(2):207-217. (IF 3.699)

35. Yanqiang Zou, Cheng Zhou, Heng Xu, Jizhang Yu, Ping Ye, Hao Zhang, Shanshan Chen, Jing Zhao, Sheng Le, Jikai Cui, Lang Jiang, Jie Wu, Jiahong Xia

Glibenclamide ameliorates transplant-induced arteriosclerosis and inhibits macrophage migration and MCP-1 expression

Life Sci. 2020 Jan 15;241:117141. doi: 10.1016/j.lfs.2019.117141. (IF 3.647)

36. Lei Peng, Chenxi Hu, Chaozheng Zhang, Yingying Lu, Shuli Man, Long Ma

Anti-cancer activity of Conyza blinii saponin against cervical carcinoma through MAPK/TGF-β/Nrf2 signaling pathways

J Ethnopharmacol. 2020 Apr 6;251:112503. doi: 10.1016/j.jep.2019.112503. (IF 3.69)

37. Songyun Deng, Lina Zhang, Yunan Mo, Yan Huang, Wenchao Li, Qianyi Peng, Li Huang, Yuhang Ai

Mdivi-1 attenuates lipopolysaccharide-induced acute lung injury by inhibiting MAPKs, oxidative stress and apoptosis

Pulm Pharmacol Ther. 2020 Jun;62:101918. doi: 10.1016/j.pupt.2020.101918. (IF 2.681)

38. Fengge Shen, Chunpo Ge, Peng Yuan

Aloe-emodin induces autophagy and apoptotic cell death in non-small cell lung cancer cells via Akt/mTOR and MAPK signaling

Eur J Pharmacol. 2020 Nov 5;886:173550. doi: 10.1016/j.ejphar.2020.173550. (IF 3.263)

39. Xiuying Shi, Xu Han, Yu Cao, Cheng Li, Yaming Cao

ZCCHC14 regulates proliferation and invasion of non-small cell lung cancer through the MAPK-P38 signalling pathway

J Cell Mol Med. 2021 Feb;25(3):1406-1414. doi: 10.1111/jcmm.16223. (IF 4.486)

40. Jiliang Yan, Sumaira Hanif, Dongya Zhang, Muhammad Ismail, Xiao Wang, Qianjin Li, Bingyang Shi, Pir Muhammad, Haigang Wu

Arsenic Prodrug-Mediated Tumor Microenvironment Modulation Platform for Synergetic Glioblastoma Therapy

ACS Appl Mater Interfaces. 2022 Aug 17;14(32):36487-36502. doi: 10.1021/acsami.2c12076.

浏览此产品的顾客也同时浏览：

p38 MAPK Rabbit Monoclonal Antibody

AF5887

50μl

Phospho-p38 MAPK (Thr180/Tyr182) Rabbit Polyclonal Antibody

AJ516

>20次

Phospho-JNK/SAPK抗体(小鼠单抗)

AF5884

50μl

Phospho-p38 MAPK (Thr180) Rabbit Polyclonal Antibody

AM071

>20次

Phospho-p44/42 MAPK抗体(小鼠单抗)

用户名：
密码：
校验码：
	后续自动登录

	找回密码注册

新冠相关产品

试剂

核酸相关

多肽与蛋白

细胞相关

信号转导

抗体

抑制剂激活剂等

常用试剂

耗材

仪器

技术服务