碧云天生物技术-EMSA探针－NF-κB (1.75μM)(GS056)

试剂

技术服务

订货电话

400-1683301

800-8283301

电话订货时间:

工作日9:00-17:00

订货邮件

order@beyotime.com

订货QQ

4001683301

订货须知

订单下载

碧云天订单.xls

资源下载

试剂> 信号转导> 转录调控> EMSA相关

EMSA探针－NF-κB (1.75μM)

一键复制产品信息

产品编号: GS056

产品包装:60μl

选择包装

60μl

数

量

说明书下载

价格: ¥ 195.00

加入购物车

				产品简介
			
				使用说明
			
				产品文件
			
				相关产品
			
				相关论文
			
				产品问答

产品编号	产品名称	产品包装	产品价格
GS056	EMSA探针－NF-κB (1.75μM)	60μl	195.00元

      EMSA探针－NF-κB是用于EMSA(也称gel shift)研究的NF-κB consensus oligonucleotide。这个双链寡核苷酸含有公认的NF-κB结合位点，可以用作EMSA研究时的探针。
      NF-κB consensus oligo的序列如下：
      5'-AGT TGA GGG GAC TTT CCC AGG C-3'
      3'-TCA ACT CCC CTG AAA GGG TCC G-5'
      本EMSA探针可以使用T4 Polynucleotide Kinase在[γ-³²P]ATP存在的情况下进行标记，也可以用于生物素标记。
      一个包装的探针可以进行约30个同位素标记探针的反应，每次标记的探针可以测定约100个样品；或约可以进行20个生物素标记探针反应。如果作为未标记的探针用于同位素标记探针的冷竞争(cold competition)，可以进行30-60个冷竞争反应。
包装清单：

产品编号	产品名称	包装
GS056	EMSA探针－NF-κB(1.75μM)	60μl
―	说明书	1份

保存条件：
-20℃保存，一年有效。
注意事项：
避免加热到40℃以上，温度过高会导致双链DNA探针解聚成单链。而单链无法用于EMSA研究。
对于EMSA的详细操作可以参考我们的EMSA试剂盒的使用说明。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 用于EMSA探针的标记，可以参考如下反应体系：

待标记探针(1.75μM)	2μl
T4 Polynucleotide Kinase 10X Buffer	1μl
Nuclease-Free Water	5μl
[γ-³²P]ATP(3,000Ci/mmol at 10mCi/ml)	1μl
T4 Polynucleotide Kinase (5-10u/μl)	1μl
总体积	10μl

2. 如果用于生物素标记，具体方法和步骤可以参考碧云天的EMSA探针生物素标记试剂盒(GS008)。
3. 如果用于冷竞争，没有标记的探针的用量应该是标记探针的用量的50-100倍。

物质安全数据单

输入右侧验证码点击下载：

下载

质检证书

输入批号搜寻COA

搜索

相关产品请点击如下按钮：

EMSA相关

使用本产品的文献：

1. Zheng Y, Liu X, Guo SW.

Therapeutic potential of andrographolide for treating endometriosis.

Hum Reprod. 2015 May;27(5):969-77. (IF 5.733)

2. Xu Y, Gao AM, Ji LJ, Li X, Zhong LL, Li HL, Zheng DH.

All-Trans Retinoic Acid Attenuates Hypoxia-Induced Injury in NRK52E Cells via Inhibiting NF-x03BA;B/VEGF and TGF-β2/VEGF Pathway.

CELL PHYSIOL BIOCHEM. 2016;38(1):229-36. (IF 5.5)

3. Heling Yu, Hengyong Xu, Chaoyang Yan, Shiliang Zhu, Xi Lan, Yuxiang Lu, Qijian He, Huadong Yin, Qing Zhu, Xiaoling Zhao, Diyan Li, Yiping Liu, Yan Wang

gga-miR-148a-5p-Targeting PDPK1 Inhibits Proliferation and Cell Cycle Progression of Avain Leukosis Virus Subgroup J (ALV-J)-Infected Cells

Front Cell Dev Biol. 2020 Dec 15;8:587889. doi: 10.3389/fcell.2020.587889. (IF 5.201)

4. Liu JS, Wei XD, Lu ZB, Xie P, Zhou HL, Chen YY, Ma JM, Yu LZ.

Liang-Ge-San, a classic traditional Chinese medicine formula, protects against lipopolysaccharide-induced inflammation through cholinergic anti-inflammatory pathway.

ONCOTARGET. 2016 Apr 19;7(16):21222-34. (IF 5.168)

5. Sun XG, Fu XQ, Cai HB, Liu Q, Li CH, Liu YW, Li YJ, Liu ZF, Song YH, Lv ZP.

Proteomic Analysis of Protective Effects of Polysaccharides from Salvia miltiorrhiza AgainstImmunological Liver Injury in Mice.

Phytother Res. 2011 Jul;25(7):1087-94. (IF 4.087)

6. Qin QJ, Niu JY, Wang ZX, Xu WJ, Qiao ZD, Gu y.

Astragalus membranaceus Inhibits Inflammation via Phospho-P38 Mitogen-Activated Protein Kinase (MAPK) and Nuclear Factor (NF)-κB Pathways in Advanced Glycation End Product-Stimulated Macrophages.

INT J MOL SCI . 2012;13(7):8379-87. (IF 3.687)

7. Xie X, Peng J, Huang K, Huang J, Shen X, Liu P, Huang H.

Polydatin ameliorates experimental diabetes-induced fibronectin through inhibiting the activationof NF-κB signaling pathway in rat glomerular mesangial cells.

Mol Cell Endocrinol. 2012 Oct 15;362(1-2):183-93. (IF 3.871)

8. Sun J, Fan L, Li M, Zhang Y, Cheng N.

Decreasing Pin1 suppresses telomerase activity by NF-κB in HCT116 cells colorectal carcinoma.

《中德临床肿瘤学杂志》. 2013 April; 12( 4):181-7.

9. Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N.

Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-κappa B signaling induced by tumor necrosis factor-α in human A549 lung epithelial cells.

PLoS One. 2014 Jun 24;9(6):e100787. (IF 2.74)

10. Guan H, Zhao L, Cao H, Chen A, Xiao J.

Epoxyeicosanoids suppress osteoclastogenesis and prevent ovariectomy-induced bone loss.

FASEB J. 2015 Mar;29(3):1092-101. (IF 4.966)

11. Guan H, Mi B, Li Y, Wu W, Tan P, Fang Z, Li J, Zhang Y, Li F.

Decitabine represses osteoclastogenesis through inhibition of RANK and NF-κB.

Cell Signal. 2015 May;27(5):969-77. (IF 3.968)

12. Shan X, Tian LL, Zhang YM, Wang XQ, Yan Q, Liu JW.

Ginsenoside Rg3 suppresses FUT4 expression through inhibiting NF-κB/p65 signaling pathway to promote melanoma cell death.

Int J Oncol. 2015 Aug;47(2):701-9. (IF 3.899)

13. Tan P, Guan H, Xie L, Mi B, Fang Z, Li J, Li F.

FOXO1 inhibits osteoclastogenesis partially by antagnozing MYC.

SCI REP-UK. 2015 Nov 16;5:16835. (IF 3.998)

14. Jiang X, Li Z, Jiang S, Tong X, Zou X, Wang W, Zhang Z, Wu L, Tian D.

Lipoxin A4 exerts protective effects against experimental acute liver failure by inhibiting the NF-κBpathway.

Int J Mol Med. 2016 Mar;37(3):773-80. (IF 3.098)

15. Zhang Y, Wu J, Ying S, Chen G, Wu B, Xu T, Liu Z, Liu X, Huang L, Shan X, Dai Y, Liang G.

Discovery of new MD2 inhibitor from chalcone derivatives with anti-inflammatory effects in LPS-inducedacute lung injury.

SCI REP-UK. 2016 Apr 27;6:25130. (IF 3.998)

16. Wu J, Hu G, Lu Y, Zheng J, Chen J, Wang X, Zeng Y.

Palmitic acid aggravates inflammation of pancreatic acinar cells by enhancing unfolded protein responseinduced CCAAT-enhancer-binding protein β-CCAAT-enhancer-binding protein α activation.

INT J BIOCHEM CELL B. 2016 Oct;79:181-193. (IF 3.673)

17. Liu C, Guan H, Cai C, Li F, Xiao J.

Lipoxin A4 suppresses osteoclastogenesis in RAW264.7 cells and prevents ovariectomy-induced bone loss.

Exp Cell Res. 2017 Mar 15;352(2):293-303. (IF 3.383)

18. Zhou J, Ma X, Shi M, Chen C, Sun Y, Li J, Xiong Y, Chen J, Li F.

Serum metabolomics analysis reveals that obvious cardioprotective effects of low dose Sini decoction against isoproterenol-induced myocardial injury in rats.

Phytomedicine. 2017 Jul 15;31:18-31. (IF 4.268)

19. Zhao L, Cai C, Wang J, Zhao L, Li W, Liu C, Guan H, Zhu Y, Xiao J.

Dihydromyricetin Protects against Bone Loss in Ovariectomized Mice by Suppressing Osteoclast Activity.

Front Pharmacol. 2017 Dec 19;8:928. (IF 4.225)

20. Chong Geng, Xiao Li, Yanni Li, Shuailing Song, Chunhui Wang

Nonsteroidal anti-inflammatory drugs alleviate severity of post-endoscopic retrograde cholangiopancreatography pancreatitis by inhibiting inflammation and reducing apoptosis

EUR J GASTROEN HEPAT. 2020 May;35(5):896-904. doi: 10.1111/jgh.15012. (IF 2.251)

21. Jiansheng Li, Yanqin Qin, Yulong Chen, Peng Zhao, Xuefang Liu, Haoran Dong, Wanchun Zheng, Suxiang Feng, Xiaoning Mao, Congcong Li

Mechanisms of the lipopolysaccharide-induced inflammatory response in alveolar epithelial cell/macrophage co-culture

Exp Ther Med. 2020 Nov;20(5):76. doi: 10.3892/etm.2020.9204. (IF 1.785)

浏览此产品的顾客也同时浏览：

GS056B

200μl

生物素标记EMSA探针－NF-κB (0.2μM)

NF-κB激活－核转运检测试剂盒(兔多抗)

GS032T

30μl

EMSA突变探针－HIF-1α (10μM)

EMSA突变探针－HIF-1α (1.75μM)

用户名：
密码：
校验码：
	后续自动登录

	找回密码注册

新冠相关产品

试剂

核酸相关

多肽与蛋白

细胞相关

信号转导

抗体

抑制剂激活剂等

常用试剂

耗材

仪器

技术服务