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多肽与蛋白> 重组蛋白> 细胞因子
Recombinant Murine G-CSF
产品编号: P6002-10μg
产品包装:10μg
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价格: ¥ 1671.00
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产品编号 产品名称 产品包装 产品价格
P6002-10μg Recombinant Murine G-CSF 10μg 1671.00元
P6002-100μg Recombinant Murine G-CSF 100μg 7987.00元
P6002-1mg Recombinant Murine G-CSF 1mg 25545.00元
Species Gene ID Accession Source Length MW Tag
Mouse 12985 P09920 E. coli 178aa 18.9kDa
About this Antibody
Name Recombinant Murine G-CSF (Recombinant Murine Granulocyte Colony Stimulating Factor; rMuG-CSF);Recombinant Mouse G-CSF ; 重组小鼠粒细胞集落刺激因子
Synonyms CSF3; C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3OS; filgrastim; Filgrastim; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin; Pluripoietin
Purity >98% by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2μm filtered concentrated solution in 10mM Sodium Citrate, pH4.0, 150mM NaCl, 0.01% Tween-20.
Endotoxin Less than 0.1EU/μg of rMuG-CSF as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category Cytokine
Background Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-α activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation and function of 2 related white cell populations of the blood, the granulocytes and the monocytesmacrophages. In addition, it may function in some adhesion or recognition events at the cell surface. The murine G-CSF cDNA encodes a 208 amino acid (a.a.) residue precursor protein containing a 30a.a. residue signal peptide that is proteolytically cleaved to generate the 178a.a. residue mature protein. Murine G-CSF is 73% identical at the amino acid level to human G-CSF and the two proteins show species cross-reactivity.
Amino Acid Sequence VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA

包装清单:

产品编号 产品名称 包装
P6002-2μg Recombinant Murine G-CSF 2μg
P6002-10μg Recombinant Murine G-CSF 10μg
P6002-100μg Recombinant Murine G-CSF 100μg
P6002-1mg Recombinant Murine G-CSF 1mg
- 说明书 1份

保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明,碧云天相关产品均为冻干粉,由于微量的蛋白在冻干过程中沉积在管内,形成很薄或不可见的蛋白层,所以在打开管盖前,我们建议在离心机中约8,000-12,000g离心10-30秒,使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的,一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子,即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡,以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化。

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