碧云天生物技术-RNase Inhibitor(RNA酶抑制剂)(R0102-2kU)

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RNase Inhibitor

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产品编号: R0102-2kU

产品包装:2000U

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产品编号	产品名称	产品包装	产品价格
R0102-2kU	RNase Inhibitor	2000U	175.00元
R0102-10kU	RNase Inhibitor	10000U	689.00元
R0102-50kU	RNase Inhibitor	50000U	2716.00元

RNase Inhibitor (Ribonuclease Inhibitor)，即RNases抑制剂，是一种大肠杆菌重组表达的人胎盘核糖核酸酶抑制剂
(human placental RNase inhibitor)，可以通过非竞争性方式按1:1比例和RNase A、RNase B、RNase C和人胎盘核糖核酸酶(human placental RNase)结合，并抑制这些酶的活性，从而保护RNA不被这些酶降解。
RNase Inhibitor对RNase A、RNase B、RNase C和人胎盘核糖核酸酶的抑制能力极强，其Ki值低至约为4×10^-14M。通常抗体和抗原的亲和常数仅为10^-6-10^-9M。并且RNase Inhibitor和这些RNA酶的结合是非常快速的，几乎在加入的瞬间就会和这
些RNA酶形成复合物从而抑制其酶活性。
RNase Inhibitor不能抑制RNase I、T1、T2、H、U1、U2、CL3、RNase from Aspergillus、S1 Nuclease、Taq DNA
polymerase、M-MLV reverse transcriptase和SP6、T7、T3 RNA polymerase的的酶活性。
RNase Inhibitor在pH 5-8范围内保持其RNA酶抑制活性，在pH 7-8时抑制活性最高。RNase Inhibitor维持其活力需要至少在溶液中含有1mM DTT。
本产品与Thermo公司的RiboLock RNase Inhibitor以及Promega公司的RNasin Ribonuclease Inhibitor属于同类产品，都是重组表达的人胎盘核糖核酸酶抑制剂，对于RNase的抑制效果也完全相同(参考图1和图2)。

图1. 碧云天的RNase Inhibitor和同类产品(Competitor RNase Inhibitor)对RNase A酶活性的抑制效果比较。5μg yeast RNA与0或2ng RNase A及8、4、2、1或0U RNase Inhibitor，在100μl的反应体系(50mM MOPS, 5mM MgCl₂, pH6.5)中，37℃孵育15分钟。反应完毕后立即取20μl反应液用1%琼脂糖凝胶进行电泳分析。

图2. 碧云天的RNase Inhibitor和同类产品(Competitor RNase Inhibitor)对RNase A的酶活性的抑制效果比较。2μg Hela细胞总RNA与0或0.5ng RNase A及20、10、5、2.5、1.25、0.625或0U RNase Inhibitor，在20μl的反应体系(50mM MOPS, 5mM MgCl₂, pH6.5)中，37℃孵育15分钟。反应完毕后立即全部用1%琼脂糖凝胶进行电泳分析。

用途：用于cDNA合成，体外转录，体外翻译，以及mRNA-protein复合物分离纯化等过程中保护RNA不被降解；还可用于特定RNase活性的鉴定等。
来源：由大肠杆菌表达，表达基因的来源为human placenta中编码该酶的基因。
分子量：约49.6 kDa(单体)。
活性定义：将能够抑制5ng RNase A的50%活性的酶量定义为一个活性单位。
活性检测条件：100mM Tris-HCl (pH7.5), 1.2mM EDTA, 0.1mg/ml BSA, 100ng/ml RNase A, 0.1mg/ml E.coli [3H]-
RNA, 50mg/ml yeast RNA, 8mM DTT。
纯度：不含DNA内切酶和外切酶，不含RNA酶。
储存溶液：20mM HEPES-KOH (pH7.5), 50 mM KCl, 5 mM DTT, 50% (v/v) glycerol。
失活或抑制：75℃加热10分钟可以导致完全失活。70℃加热10分钟仍会有微量活性残留。SDS和尿素等导致蛋白变性的试剂
以及对氯汞基苯甲酸盐(p-chloromercuribenzoate)、重铬酸钾等氧化剂会抑制RNase Inhibitor与RNA酶的结合。
关于碧云天RNase Inhibitor的比较和选择，请参考碧云天的相关网页：https://www.beyotime.com/support/rnase-inhibitor.htm。
包装清单：

产品编号	产品名称	包装
R0102-2KU	RNase Inhibitor (40U/μl)	2000U
R0102-10KU	RNase Inhibitor (40U/μl)	10000U
R0102-50KU	RNase Inhibitor (40U/μl)	50000U
－	说明书	1份

保存条件：
-20℃保存。
注意事项：
使用时宜存放在冰盒内或冰浴上，使用完毕后宜立即放置于-20℃保存。
酶储存溶液中加入DTT可保证RNase Inhibitor长期贮存的稳定性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 对于cDNA合成、体外转录、体外翻译等常见的反应体系中，为保护其中的RNA不被RNase降解，RNase Inhibitor推荐用量为最终浓度1-2U/μl。

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