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 ATP检测试剂盒

  产品编号S0026
  产品包装: 200次
  产品价格: 927.00元
产品编号 产品名称 产品包装 产品价格
S0026 ATP检测试剂盒 200次 927.00元

      ATP检测试剂盒(ATP Assay Kit)可以用于检测普通溶液、细胞或组织内的ATP(adenosine 5'-triphosphate)水平。细胞和组织样品一步裂解即可完成样品制备,检测灵敏度高达1nM,化学发光可以持续稳定达30分钟,并且获得的样品还可以同时进行Western检测。
      ATP,作为最重要的能量分子,在细胞的各种生理、病理过程中起着重要作用。ATP水平的改变,会影响细胞的功能。通常细胞在凋亡、坏死或处于一些毒性状态下,ATP水平会下降;而高葡萄糖刺激等可上调某些细胞的细胞内ATP水平。通常ATP水平的下降表明线粒体的功能受损或下降,在细胞凋亡时ATP水平的下降通常和线粒体的膜电位下降同时发生。
      本试剂盒根据萤火虫萤光素酶(firefly luciferase,也称荧光素酶)催化萤光素产生萤光时需要ATP提供能量研制而成。当萤火虫萤光素酶和萤光素都过量时,在一定的浓度范围内萤光的产生和ATP的浓度成正比。这样就可以高灵敏地检测溶液中的ATP浓度。
      样品制备简单。本试剂盒提供了可以用于细胞和组织裂解的ATP检测裂解液,简单裂解后即可用于ATP检测。无需进行高氯酸或三氯乙酸(TCA)抽提,或样品裂解后的煮沸等繁琐操作。
      灵敏度高,线性范围宽,在1nM至10μM范围内有良好检测效果。本试剂盒在样品体积为100微升时可以检测浓度低达1nM的ATP。而常规的细胞或组织裂解液中ATP的浓度为0.1-1μM,一些常见细胞的细胞内ATP水平约为10nmol/mg蛋白。并且本试剂盒的检测浓度范围非常宽,检测上限可以高达10μM,并在1nmol/L-10μM范围内可以形成良好的标准曲线。
      读数稳定。本试剂盒进行了特殊的优化设计,使检测ATP时的化学发光非常稳定。对于ATP标准曲线的检测结果显示,在开始反应后10分钟内化学发光无明显下降,开始反应后30分钟内化学发光的下降不超过10%。
      制备的样品兼容性好。使用本试剂盒中的ATP检测裂解液裂解获得的细胞或组织样品,不仅可以用于ATP检测,还可用于检测蛋白浓度、进行SDS-PAGE或一些常规的较易溶解蛋白的Western检测。
      使用方便快捷。通常10-20个样品可以在30-60分钟内测定完毕。
      本ATP检测试剂盒的检测灵敏度低于S0027, 但高于S0026B;本试剂盒对于一些特殊样品的兼容性低于S0026B
      国际顶级期刊Cell发表的论文中注明使用了本产品(Cell. 2014 Jul 31;158(3):607-19)。
      一个包装的本试剂盒至少可以检测200个样品。
包装清单:

产品编号 产品名称 包装
S0026-1 ATP检测试剂 2ml(0.5ml/管,共4管)
S0026-2 ATP检测试剂稀释液 20ml
S0026-3 ATP标准溶液(0.5mM) 0.1ml
S0026-4 ATP检测裂解液 100ml
说明书 1份

保存条件:
      -20℃保存,半年有效。-70℃保存,一年有效。ATP检测试剂需避光保存。
注意事项:
      ATP检测试剂中含有萤光素酶,反复冻融会导致其逐渐失活。尽管经测试ATP检测试剂反复冻融5次对于其检测效果无明显影响,为取得较好的使用效果,建议用户使用时的冻融次数不宜超过3次。ATP检测试剂稀释成ATP检测工作液后,最好一次用完,不宜冻存后再使用。
      ATP,特别是裂解后样品中的ATP在室温不太稳定,需在4或冰上操作。ATP在冰上可以稳定长达6小时。
      本试剂盒需使用luminometer,即化学发光仪(检测萤光素酶报告基因时所用的仪器)。如果没有luminometer,也可以使用液闪仪。液闪仪的测定效果取决于液闪仪的检测灵敏度和检测精度。
      使用可检测化学发光的多功能酶标仪时,推荐使用孔和孔之间不透光的96孔白板或黑板。如使用普通的透明96孔板,须特别注意在检测孔之间设置间隔孔,以减少邻近孔之间的相互干扰。对于透明96孔板,一个发光孔可以使上下或左右邻近孔的RLU值升高该孔的10-20%左右,使上下或左右间隔一个孔的邻近孔的RLU值升高该孔的1%-4%左右;对于相同的样品,底部不透光的96孔白板的化学发光读数可以达到透明96孔板的5-10倍左右,达到底部透光96孔白板读数的3倍左右(实测数据会因96孔板、检测仪器和样品等的不同而存在差异)。
      本试剂盒提供的ATP检测裂解液可以有效裂解并释放常见的培养细胞和组织中的ATP。对于一些特殊的组织或样品,如果发现检测出来的ATP水平显著低于预期水平,可以在裂解样品后并且在离心前,取部分样品煮沸2分钟以充分释放ATP。煮沸后样品中的蛋白会变性,从而会在后续的离心步骤中被沉淀,因此煮沸的样品不能用于蛋白浓度测定、SDS-PAGE和Western检测。可以使用剩余的部分样品进行蛋白浓度测定、SDS-PAGE和Western检测。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 样品测定的准备:(注意:样品裂解需在4℃或冰上操作)
对于贴壁细胞:
吸除培养液,按照6孔板每孔加入200微升裂解液的比例(即相当于细胞培养液量2毫升的1/10)加入裂解液,裂解细胞。裂解细胞时为了裂解充分,可以使用移液器进行反复吹打或晃动培养板使裂解液充分接触并裂解细胞。通常细胞在接触裂解液后会立即裂解。裂解后4℃ 12000g离心5分钟,取上清,用于后续的测定。
对于悬浮细胞:
用离心管离心沉淀细胞,弃上清,轻轻弹散细胞,按照6孔板每孔的细胞量加入200微升裂解液的比例加入裂解液,裂解细胞。裂解细胞时为了裂解充分可以弹击离心管管底或适当Vortex使裂解液充分接触并裂解细胞。通常细胞在接触裂解液后会立即裂解。裂解后4℃ 12000g离心5分钟,取上清,用于后续的测定。
对于组织样品:
按照每20毫克组织加入约100-200微升裂解液的比例加入裂解液,然后用玻璃匀浆器或其它匀浆设备进行匀浆。充分匀浆可以确保组织被完全裂解。裂解后4℃ 12000g离心5分钟,取上清,用于后续的测定。
2. 标准曲线测定的准备:
冰浴上融解待用试剂,把ATP标准溶液用ATP检测裂解液稀释成适当的浓度梯度。具体的浓度需根据样品中ATP的浓度而定。初次检测可以检测0.01、0.03、0.1、0.3、1、3和10μM这几个浓度,在后续的实验中,可以根据样品中ATP的浓度对标准品的浓度范围进行适当调整。
3. ATP检测工作液的配制:
按照每个样品或标准品需100微升ATP检测工作液的比例配制适当量的ATP检测工作液。把待用试剂在冰浴上融解。取适量的ATP检测试剂,按照1:9的比例用ATP检测试剂稀释液稀释ATP检测试剂。例如100微升ATP检测试剂加入900微升ATP检测试剂稀释液配制成1毫升ATP检测工作液。稀释后的ATP检测试剂即为用于后续实验的ATP检测工作液。ATP检测工作液可在冰浴上暂时保存。
4. ATP浓度的测定:
a. 加100微升ATP检测工作液到检测孔或检测管内。室温放置3-5分钟,以使本底性的ATP全部被消耗掉,从而降低本底。可以一次性把10-20个检测孔或检测管分别加上100微升ATP检测工作液,从而节省时间。
b. 在检测孔或检测管内加上20微升样品或标准品,迅速用枪(微量移液器)混匀,至少间隔2秒后,用化学发光仪(luminometer)或液闪仪测定RLU值或CPM。(注:样品的体积可以自行在10-100微升范围内调节。如果样品中的ATP浓度比较低则可以加入100微升样品,如果样品中ATP浓度比较高则可以加入较小体积的样品,同时标准品也需要使用相同的体积。如果样品中ATP的浓度特别高,可以用ATP检测裂解液稀释样品后再测定。本试剂盒在加入10-100微升标准品时,大致在1nM-10μM的浓度范围内有很好的线性关系(参考图1)。

图1. 本产品对ATP标准品的检测效果。图中数据为20微升标准品并减去空白对照后的数据。实测数据会因检测仪器等的不同而存在差异,图中数据仅供参考。

c. 根据标准曲线计算出样品中ATP的浓度。
d. 为了消除样品制备时由于蛋白量的差异而造成的误差,可以用碧云天生产的BCA蛋白浓度测定试剂盒
(
P0009/P0010/P0010S/P0011/P0012/P0012S)测定样品中的蛋白浓度。然后把ATP的浓度换算nmol/mg蛋白的形式。

常见问题:
1. Luminometer和荧光分光光度计有何不同?
荧光分光光度计检测的样品本身不能发光,样品需要由特定波长的激发光激发,然后才能产生荧光并被荧光分光光度计检测。Luminometer,即化学发光检测仪,检测的样品本身可以发光,不需要激发光进行激发。也就是说Luminometer是检测化学发光(萤光)的仪器。有些型号的荧光分光光度计也具有luminometer的功能,即也可以检测化学发光。您所使用的荧光分光光度计能否用于化学发光的测定请仔细阅读该仪器的说明书。
2. 可以进行萤光素酶报告基因检测的仪器是否就可以用于本试剂盒的ATP检测?
是。萤光素酶报告基因的检测原理和本ATP检测试剂盒的原理相同,可以用相同的仪器测定,并且可以选择相同的测定参数,例如检测前等待时间为2秒,检测时间为10秒等。
3. 多功能酶标仪是否可以用于本试剂盒的ATP检测?
不一定。如果该多功能酶标仪具有检测化学发光的功能,即具有luminometer的功能,就可以用于本试剂盒的检测,否则就不能了。


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Role of poly(ADP-ribose) glycohydrolase in the regulation of cell fate in response tobenzo(a)pyrene.
Exp Cell Res. 2012 Mar 10;318(5):682-90.
27.Yuan Y, Ming Z, Gong-Hua H, Lan G, Lu D, Peng L, Feng J, Cai-Gao Z.
Cr(VI) induces the decrease of ATP level and the increase of apoptosis rate mediated by ROS orVDAC1 in L-02 hepatocytes.
Environ Toxicol Pharmacol. 2012 Sep;34(2):579-87.
28.Ji X, Gao Y, Chen L, Zhang Z, Deng Y, Li Y.
Nanohybrid systems of non-ionic surfactant inserting liposomes loading paclitaxel for  reversal of multidrugresistance.
Int J Pharm. 2012 Jan 17;422(1-2):390-7.
29.Liu YQ, Cheng X, Guo LX, Mao C, Chen YJ, Liu HX, Xiao QC, Jiang S, Yao ZJ, Zhou GB.
Identification of an annonaceous acetogenin mimetic, AA005, as an AMPK activator and autophagy inducer in colon cancer cells.
PLoS One. 2012;7(10):e47049.
30.Yue R, Hu H, Yiu KH, Luo T, Zhou Z, Xu L, Zhang S, Li K, Yu Z.
Lycopene protects against hypoxia/reoxygenation-induced apoptosis by preventing mitochondrial dysfunction inprimary neonatal mouse cardiomyocytes.
PLoS One. 2012;7(11):e50778. 
31.Peng Y, Yang J, Zhang E, Sun H, Wang Q, Wang T, Su Y, Shi C.
Human positive coactivator 4 is a potential novel therapeutic target in non-small cell lung cancer.
Cancer Gene Ther. 2012 Oct;19(10):690-6.
32.Lu Y, Lai Q , Zhang C, Zhao H, Xing X.
Alteration of energy metabolism in Enterobacter aerogenes by external addition of  pyrophosphates and overexpression of polyphosphate kinase for enhanced hydrogen  production.
Journal of Chemical Technology and Biotechnology. 2012 Jul;87(7):996–1003.
33.Wei Z, Yuan S, Hao J, Fang X.
Mechanism of inhibition of P-glycoprotein mediated efflux by Pluronic P123/F127 block copolymers: Relationship between copolymer concentration and inhibitory activity.
Eur J Pharm Biopharm. 2013 Feb;83(2):266-74.
34.Ma C, Tang Z, Wang K, Yang X, Tan W.
A novel sensitive and selective ligation-based ATP assay using a molecular beacon.
Analyst. 2013 Apr 22;138(10):3013-7.
35.Tang Y, Wang F, Jin C, Liang H, Zhong X, Yang Y.
Mitochondrial injury induced by nanosized titanium dioxide in A549 cells and rats.
Environ Toxicol Pharmacol. 2013 Jul;36(1):66-72.
36.Wang X, Bai H, Zhang X, Liu J, Cao P, Liao N, Zhang W, Wang Z, Hai C.
Inhibitory effect of oleanolic acid on hepatocellular carcinoma via ERK-p53-mediated  cell cycle arrest andmitochondrial-dependent apoptosis.
Carcinogenesis.2013 Jun;34(6):1323-30.
37.Wang K, Gao F, Ji Y, Liu Y, Dan Z, Yang P, Zhu Y, Li S.
ORFH79 impairs mitochondrial function via interaction with a subunit of electron  transport chain complex III inHonglian cytoplasmic male sterile rice.
New Phytol. 2013 Apr;198(2):408-18. 
38.Wang J, Chen Y, Zhang W, Zheng G, Meng S, Che H, Ke T, Yang J, Chen J, Luo W.
Akt Activation Protects Liver Cells from Apoptosis in Rats during Acute Cold Exposure.
Int. J. Biol. Sci. 2013;9(5):509-17. 
39.Li N, Zhang CX, Wang XX, Zhang L, Ma X, Zhou J, Ju RJ, Li XY, Zhao WY, Lu WL.
Development of targeting lonidamine liposomes that circumvent drug-resistant cancer by acting on mitochondrialsignaling pathways.
Biomaterials. 2013 Apr;34(13):3366-80.
40.Li X, Long D, Ji J, Yang W, Zeng Z, Guo S, Ji Z, Qi G, Chen S.
Sample preparation for the metabolomics investigation of poly-gamma-glutamate-producing Bacillus licheniformisby GC-MS.
J Microbiol Methods. 2013 Jul;94(1):61-7.
41.Dong LY, Jin J, Lu G, Kang XL.
Astaxanthin Attenuates the Apoptosis of Retinal Ganglion Cells in db/db Mice by Inhibition of Oxidative Stress.
Mar Drugs. 2013 Mar 21;11(3):960-74. 
42.Qi M, Fan S, Yao G, Li Z, Zhou H, Tashiro S, Onodera S, Xia M, Ikejima T.
Pseudolaric acid B-induced autophagy contributes to senescence via enhancement of ROS generation and mitochondrial dysfunction in murine fibrosarcoma L929 cells.
J Pharmacol Sci. 2013;121(3):200-11.
43.Yin Q, Shen J, Zhang Z, Yu H, Chen L, Gu W, Li Y.
Multifunctional Nanoparticles Improve Therapeutic Effect for Breast Cancer by Simultaneously AntagonizingMultiple Mechanisms of Multidrug Resistance.
Biomacromolecules. 2013 Jul 8;14(7):2242-52.
44.Zhang X, Zuo X, Yang B, Li Z, Xue Y, Zhou Y, Huang J, Zhao X, Zhou J, Yan Y, Zhang H,Guo P, Sun H, Guo L, Zhang Y, Fu XD.
MicroRNA directly enhances mitochondrial translation during muscle differentiation. differentiation.
Cell. 2014 Jul 31;158(3):607-19.
45.Cui J, He W, Yi B, Zhao H, Lu K, Ruan H, Ma D.
mTOR pathway is involved in ADP-evoked astrocyte activation and ATP release in the spinal dorsal horn in a rat neuropathic pain model.
Neuroscience. 2014 Sep 5;275:395-403. 
46.Xie X, Xu XM, Li N, Zhang YH, Zhao Y, Ma CY, Dong DL.
DMH1 increases glucose metabolism through activating Akt in L6 rat skeletal muscle cells.
PLoS One. 2014 Sep 23;9(9):e107776. 
47.Meng G, Xia M, Xu C, Yuan D, Schnurr M, Wei J.
Multifunctional antitumor molecule 5'-triphosphate siRNA combining glutaminase silencing  and RIG-I activation.
Int J Cancer. 2014 Apr 15;134(8):1958-71. 
48.Sun S, Han Y, Liu J, Fang Y, Tian Y, Zhou J, Ma D, Wu P.
Trichostatin A targets the mitochondrial respiratory chain, increasing mitochondrial reactive oxygen species production to trigger apoptosis in human breast cancer cells.
PLoS One. 2014 Mar 13;9(3):e91610.
49.Xie Y, Li Q, Yang Q, Yang M, Zhang Z, Zhu L, Yan H, Feng R, Zhang S, Huang C, Liu Z, Wen T.
Overexpression of DCF1 inhibits glioma through destruction of mitochondria and activation of apoptosis pathway.
Sci Rep. 2014 Jan 15;4:3702.
50.Su B, Ji YS, Sun XL, Liu XH, Chen ZY.
Brain-derived neurotrophic factor (BDNF)-induced mitochondrial motility arrest and  presynaptic docking contribute to BDNF-enhanced synaptic transmission.
J Biol Chem. 2014 Jan 17;289(3):1213-26.
51.Jia WC, Liu G, Zhang CD, Zhang SP.
Formononetin attenuates hydrogen peroxide (H2O2)-induced apoptosis and NF-κB activation in RGC-5 cells.
Eur Rev Med Pharmacol Sci. 2014;18(15):2191-7.
52.Zhang H, Sun R, Liu XY, Shi XM, Wang WF, Yu LG, Guo XL.
A tetramethylpyrazine piperazine derivate CXC137 prevents cell injury in SH-SY5Y cells and improves memory dysfunction of rats with vascular Dementia.
Neurochem Res. 2014 Feb;39(2):276-86. 
53.Bu H, He X, Zhang Z, Yin Q, Yu H, Li Y.
A TPGS-incorporating nanoemulsion of paclitaxel circumvents drug resistance in breast cancer.
Int J Pharm. 2014 Aug 25;471(1-2):206-13.
54.Chen G, Dai J, Tan S, Meng S, Liu Z, Li M, Cui Q, Yu M.
MTERF1 regulates the oxidative phosphorylation activity and cell proliferation in HeLa cells.
Acta Biochim Biophys Sin (Shanghai). 2014 Jun;46(6):512-21. 
55.He Y, Li H, He J, Zhao X.
Heavy ion radiation can promote greater motility and enolase protein expression in ram sperm in in vitro liquid storage.
Anim Reprod Sci. 2014 Aug;148(3-4):260-6.
56.Li Q, Zhang T, Wang J, Zhang Z, Zhai Y, Yang GY, Sun X.
Rapamycin attenuates mitochondrial dysfunction via activation of mitophagy in experimental ischemic stroke.
Biochem Biophys Res Commun. 2014 Feb 7;444(2):182-8. 
57.He Z, Liu J, Du L.
The unexpected effect of PEGylated gold nanoparticles on the primary function of erythrocytes.
Nanoscale. 2014 Aug 7;6(15):9017-24. 
58.Xia M, Meng G, Jiang A, Chen A, Dahlhaus M, Gonzalez P, Beltinger C, Wei J.
Mitophagy switches cell death from apoptosis to necrosis in NSCLC cells treated with oncolytic measles virus.
Oncotarget. 2014 Jun 15;5(11):3907-18.
59.Shen Y, Yang J, Li J, Shi X, Ouyang L, Tian Y, Lu J.
Carnosine inhibits the proliferation of human gastric cancer SGC-7901 cells through both of the mitochondrial respiration and glycolysis pathways.
PLoS One. 2014 Aug 12;9(8):e104632. 
60.Jiang XR, Yu XY, Fan JH, Guo L, Zhu C, Jiang W, Lu SH.
RFT2 is overexpressed in esophageal squamous cell carcinoma and promotes tumorigenesis by sustaining cell proliferation and protecting against cell death.
Cancer Lett. 2014 Oct 10;353(1):78-86.
61.Li J, Ke W, Zhou Q, Wu Y, Luo H, Zhou H, Yang B, Guo Y, Zheng Q, Zhang Y.
Tumour necrosis factor-α promotes liver ischaemia-reperfusion injury through the PGC-1α/Mfn2 pathway.
J Cell Mol Med. 2014 Sep;18(9):1863-73. 
62.Li X, Gou X, Long D, Ji Z, Hu L, Xu D, Liu J, Chen S.
Physiological and metabolic analysis of nitrate reduction on poly-gamma-glutamic acid synthesis in Bacillus licheniformis WX-02.
Arch Microbiol. 2014 Nov;196(11):791-9. 
63.Liu B, Tang M, Han Z, Li J, Zhang J, Lu P, Song N, Wang Z, Yin C, Zhang W.
Co-Incubation of Human Spermatozoa with Anti-VDAC Antibody Reduced Sperm Motility.
Cell Physiol Biochem. 2014;33(1):142-50. 
64.Lu L, Han H, Tian Y, Li W, Zhang J, Feng M, Li Y.
Aurora kinase A mediates c-Myc's oncogenic effects in hepatocellular carcinoma.
Mol Carcinog. 2015 Nov;54(11):1467-79.
65.Chen Y, Zhang Z, Yang K, Du J, Xu Y, Liu S.
Myeloid zinc-finger 1 (MZF-1) suppresses prostate tumor growth through enforcing ferroportin-conducted iron egress.
Oncogene. 2015 Jul;34(29):3839-47.
66.Yan H, Zhang D, Hao S, Li K, Hang CH.
Role of Mitochondrial Calcium Uniporter in Early Brain Injury After Experimental  Subarachnoid Hemorrhage.
Mol Neurobiol. 2015 Dec;52(3):1637-47. 
67.Liang M, Jin S, Wu DD, Wang MJ, Zhu YC.
Hydrogen sulfide improves glucose metabolism and prevents hypertrophy in cardiomyocytes.
Nitric Oxide. 2015 Apr 30;46:114-22.
68.Wen F, Li B, Huang C, Wei Z, Zhou Y, Liu J, Zhang H.
MiR-34a is Involved in the Decrease of ATP Contents Induced by Resistin Through Target on ATP5S in HepG2 Cells.
Biochem Genet. 2015 Dec;53(11-12):301-9.
69.Zheng B, Zhao D, Zhang P, Shen C, Guo Y, Zhou T, Guo X, Zhou Z, Sha J.
Quantitative Proteomics Reveals the Essential Roles of Stromal Interaction Molecule 1 (STIM1) in the Testicular Cord Formation in Mouse Testis.
Mol Cell Proteomics. 2015 Oct;14(10):2682-91.
70.Zhou R, Wang R, Qin Y, Ji J, Xu M, Wu W, Chen M, Wu D, Song L, Shen H, Sha J, Miao D, Hu Z, Xia Y, Lu C, Wang X.
Mitochondria-related miR-151a-5p reduces cellular ATP production by targeting CYTB in asthenozoospermia.
Sci Rep. 2015 Dec 2;5:17743.
71.Qiu M, Chen L, Tan G, Ke L, Zhang S, Chen H, Liu J.
A reactive oxygen species activation mechanism contributes to JS-K-induced apoptosis in human bladder cancer cells.
Sci Rep. 2015 Oct 13;5:15104.
72.Zhang J, Song J, Wu D, Wang J, Dong W.
Hesperetin induces the apoptosis of hepatocellular carcinoma cells via mitochondrial pathway mediated by the increased intracellular reactive oxygen species, ATP and calcium.
Med Oncol. 2015 Apr;32(4):101.
73.Huang Q, Huang J, Zeng Z, Luo J, Liu P, Chen S, Liu B, Pan X, Zang L, Zhou S.
Effects of ERK1/2/PPARα/SCAD signal pathways on cardiomyocyte hypertrophy induced by insulin-like growth factor 1 and phenylephrine.
Life Sci. 2015 Mar 1;124:41-9.
74.Wang G, Kang N, Gong H, Luo Y, Bai C, Chen Y, Ji X, Huang C, Dong Q.
Upregulation of uncoupling protein Ucp2 through acute cold exposure increases post-thaw sperm quality in zebrafish.
Cryobiology. 2015 Dec;71(3):464-71.
75.Li C, Meng G, Su L, Chen A, Xia M, Xu C, Yu D, Jiang A, Wei J.
Dichloroacetate blocks aerobic glycolytic adaptation to attenuated measles virus and promotes viral replication leading to enhanced oncolysis in glioblastoma.
Oncotarget. 2015 Jan 30;6(3):1544-55.
76.Wu D, Hu Q, Liu X, Pan L, Xiong Q, Zhu YZ.
Hydrogen sulfide protects against apoptosis under oxidative stress through SIRT1 pathway in H9c2 cardiomyocytes.
Nitric Oxide. 2015 Apr 30;46:204-12.
77.Wang S, Yang Y, Wang Y, Chen M.
Gambogic acid-loaded pH-sensitive mixed micelles for overcoming breast cancer resistance.
International Journal of Pharmaceutics. 2015 Nov 30;495(2):840-8.
78.Liu X, Chen Z, Xu C, Leng X, Cao H, Ouyang G, Xiao W.
Repression of hypoxia-inducible factor α signaling by Set7-mediated methylation.
Nucleic Acids Res. 2015 May 26;43(10):5081-98.
79.Ye W, Lei Y, Yu M, Xu Y, Cao M, Yu L, Zhang L, Li P, Bai W, Xu Z, Zhang F1.
NLRP3 inflammasome is responsible for Hantavirus inducing interleukin-1β in THP-1 cells.
Int J Mol Med. 2015 Jun;35(6):1633-40.
80.Cai R, Xue W, Liu S, Petersen RB, Huang K, Zheng L.
Overexpression of glyceraldehyde 3-phosphate dehydrogenase prevents neurovascular degeneration after retinal injury.
FASEB J. 2015 Jul;29(7):2749-58.
81.Mao J, Li Z, Lin R, Zhu X, Lin J, Peng J, Chen L.
Preconditioning with Gua Lou Gui Zhi decoction enhances H2O2-induced Nrf2/HO-1 activation in PC12 cells.
Exp Ther Med. 2015 Sep;10(3):877-84.
82.Wang Y, Yu H, Zhang J, Ge X, Gao J, Zhang Y, Lou G.
Anti-tumor effect of emodin on gynecological cancer cells.
Cell Oncol (Dordr). 2015 Oct;38(5):353-63.
83.Sun W, Xie Z, Liu Y, Zhao D, Wu Z, Zhang D, Lv H, Tang S, Jin N, Jiang H, Tan M, Ding J, Luo C, Li J, Huang M, Geng M.
JX06 Selectively Inhibits Pyruvate Dehydrogenase Kinase PDK1 by a Covalent Cysteine Modification.
Cancer Res. 2015 Nov 15;75(22):4923-36.
84.Qian S, Lu H, Meng P, Zhang C, Lv F, Bie X, Lu Z.
Effect of inulin on efficient production and regulatory biosynthesis of bacillomycin D in Bacillus subtilis fmbJ.
Bioresour Technol. 2015 Mar;179:260-7.
85.Zheng Y, Huang J, Tao L, Shen Z, Li H, Mo F, Wang X, Wang S, Shen H.
Corticosterone increases intracellular Zn(2+) release in hippocampal HT-22 cells.
Neurosci Lett. 2015 Feb 19;588:172-7.
86.Wang X, Che H, Zhang W, Wang J, Ke T, Cao R, Meng S, Li D, Weiming O, Chen J, Luo W.
Effects of Mild Chronic Intermittent Cold Exposure on Rat Organs.
Int J Biol Sci. 2015 Aug 8;11(10):1171-80.
87.Guo L, Jing J, Feng YM, Yao B.
Tamoxifen is a potent antioxidant modulator for sperm quality in patients with idiopathic oligoasthenospermia.
Int Urol Nephrol. 2015 Sep;47(9):1463-9.
88.Wu F, Liu Y, Luo L, Lu Y, Yew DT, Xu J, Guo K.
Platelet mitochondrial dysfunction of DM rats and DM patients.
Int J Clin Exp Med. 2015 May 15;8(5):6937-46. 
89.Ju R, Guo L, Li J, Zhu L, Yu X, Chen C, Chen W, Ye C, Zhang D. 
Carboxyamidotriazole inhibits oxidative phosphorylation in cancer cells and exerts synergistic anti-cancer effect with glycolysis inhibition.
Cancer Lett. 2016 Jan 28;370(2):232-41.
90.Liu Z, Sun Y, Tan S, Liu L, Hu S, Huo H, Li M, Cui Q, Yu M. 
Nutrient deprivation-related OXPHOS/glycolysis interconversion via HIF-1α/C-MYC pathway in U251 cells.
Tumour Biol. 2016 May;37(5):6661–71. 
91.Lv S, Xu Q, Sun E, Zhang J, Wu D.
Impaired cellular energy metabolism contributes to bluetongue-virus-induced autophagy.
Arch Virol. 2016 Oct;161(10):2807-11.
92.Peng X, Gan J, Wang Q, Shi Z, Xia X.
3-Monochloro-1,2-propanediol (3-MCPD) induces apoptosis via mitochondrial oxidative phosphorylationsystem impairment and the caspase cascade pathway.
Toxicology. 2016 Nov 30;372:1-11.
93.Lu Y, Zhang R, Liu S, Zhao Y, Gao J, Zhu L.
ZT-25,a new vacuolar H(+)-ATPase inhibitor, induces apoptosis and protective autophagy through ROSgeneration in HepG2 cells.
Eur J Pharmacol. 2016 Jan 15;771:130-8.
94.Wu D, Zhang J, Wang J, Li J, Liao F, Dong W.
Hesperetin induces apoptosis of esophageal cancer cells via mitochondrial pathway mediated by theincreased intracellular reactive oxygen species.
Tumour Biol. 2016 Mar;37(3):3451-9.
95.Jiao Y, Ma S, Wang Y, Li J, Shan L, Liu Q, Liu Y, Song Q, Yu F, Yu H, Liu H, Huang L, Chen J.
N-Acetyl Cysteine Depletes Reactive Oxygen Species and Prevents Dental Monomer-Induced IntrinsicMitochondrial Apoptosis In Vitro in Human Dental Pulp Cells.
PLoS One. 2016 Jan 25;11(1):e0147858.
96.Zhang J, Shi R, Li H, Xiang Y, Xiao L, Hu M, Ma F, Ma CW, Huang Z.
Antioxidant and neuroprotective effects of Dictyophora indusiata polysaccharide in Caenorhabditis elegans.
J Ethnopharmacol. 2016 Nov 4;192:413-422.
97.Hu L, Wang H, Huang L, Zhao Y, Wang J.
The Protective Roles of ROS-Mediated Mitophagy on 125I Seeds Radiation Induced Cell Death in HCT116Cells.
Oxid Med Cell Longev. 2016;2016:9460462.
98.Xu C, Liu Q, Liu H, Zhang C, Shao W, Gu A.
Toxicological assessment of multi-walled carbon nanotubes in vitro: potential mitochondria effects on malereproductive cells.
Oncotarget. 2016 Jun 28;7(26):39270-39278.
99.Ding GC, Chen M, Wang YX, Rui C, Xu W, Ding HJ, Shi ZH.
MicroRNA-128a-induced apoptosis in HTR-8/SVneo trophoblast cells contributes to pre-eclampsia.
Biomed Pharmacother. 2016 Jul;81:63-70.
100.Ju L, Tong W, Qiu M, Shen W, Sun J, Chen Y, Li Z, Wang W, Tian J.
Endurance exercise ameliorates low birthweight developed catch-up growth related metabolic dysfunctionsin a mouse model.
Endocr J. 2016;63(3):275-85.
101.Jiao Y, Ma S, Wang Y, Li J, Shan L, Sun J, Chen J.
Methacryloxylethyl Cetyl Ammonium Chloride Induces DNA Damage and Apoptosis in Human Dental PulpCells via Generation of Oxidative Stress.
Int J Biol Sci. 2016 Mar 21;12(5):580-93.
102.Wu WB, Menon R, Xu YY, Zhao JR, Wang YL, Liu Y, Zhang HJ.
Downregulation of peroxiredoxin-3 by hydrophobic bile acid induces mitochondrial dysfunction and cellular senescence in human trophoblasts.
Sci Rep. 2016 Dec 13;6:38946.
103.Sun R, Cao M, Zhang J, Yang W, Wei H, Meng X, Yin L, Pu Y.
Benzene Exposure Alters Expression of Enzymes Involved in Fatty Acid β-Oxidation in Male C3H/He Mice.
Int J Environ Res Public Health. 2016 Oct 31;13(11). pii: E1068.
104.Ju R, Guo L, Li J, Zhu L, Yu X, Chen C, Chen W, Ye C, Zhang D.
Carboxyamidotriazole inhibits oxidative phosphorylation in cancer cells and exerts synergistic anti-cancereffect with glycolysis inhibition.
Cancer Lett. 2016 Jan 28;370(2):232-41.
105.Liu Z, Sun Y, Tan S, Liu L, Hu S, Huo H, Li M, Cui Q, Yu M.
Nutrient deprivation-related OXPHOS/glycolysis interconversion via HIF-1α/C-MYC pathway in U251 cells.
Tumour Biol. 2016 May;37(5):6661-71.
106.Zhang D, Fei Q, Li J, Zhang C, Sun Y, Zhu C, Wang F, Sun Y.
2-Deoxyglucose Reverses the Promoting Effect of Insulin on Colorectal Cancer Cells In Vitro.
PLoS One. 2016 Mar 3;11(3):e0151115.
107.Li K, Zhang J, Ji C, Wang L.
MiR-144-3p and Its Target Gene β-Amyloid Precursor Protein Regulate 1-Methyl-4-Phenyl- 1,2-3,6-Tetrahydropyridine-Induced Mitochondrial Dysfunction.
Mol Cells. 2016 Jul;39(7):543-9.
108.Geng J, Li J, Huang T, Zhao K, Chen Q, Guo W, Gao J.
A novel manganese complex selectively induces malignant glioma cell death by targeting mitochondria.
Mol Med Rep. 2016 Sep;14(3):1970-8.
109.Han Y, Sun S, Zhao M, Zhang Z, Gong S, Gao P, Liu J, Zhou J, Ma D, Gao Q, Wu P.
CYC1 Predicts Poor Prognosis in Patients with Breast Cancer.
Dis Markers. 2016;2016:3528064.
110.Hao XD, Chen ZL, Qu ML, Zhao XW, Li SX, Chen P.
Decreased Integrity, Content, and Increased Transcript Level of Mitochondrial DNA Are Associated withKeratoconus.
PLoS One. 2016 Oct 26;11(10):e0165580.
111.Zeng Z, Huang Q, Shu Z, Liu P, Chen S, Pan X, Zang L, Zhou S.
Effects of short-chain acyl-CoA dehydrogenase on cardiomyocyte apoptosis.
J Cell Mol Med. 2016 Jul;20(7):1381-91.
112.Li Z, Liu X, Wang L, Wang Y, Du C, Xu S, Zhang Y, Wang C, Yang C.
The role of PGC-1α and MRP1 in lead-induced mitochondrial toxicity in testicular Sertoli cells.
Toxicology. 2016 Apr 29;355-356:39-48.
113.Jiao Y, Zhang J, Lu L, Xu J, Qin L.
The Fto Gene Regulates the Proliferation and Differentiation of Pre-Adipocytes in Vitro.
Nutrients. 2016 Feb 19;8(2):102.
114.Li JP, Ma Q, Chen CM.
Mitochondrial dysfunction in resveratrol-induced apoptosis in QGY-7701 cells.
Genet Mol Res. 2016 Mar 24;15(1).doi: 10.4238/gmr.15017490.
115.Cui J, Huang D, Zheng Y.
Ameliorative effects of α-lipoic acid on high-fat diet-induced oxidative stress and glucose uptake impairmentof T cells.
Free Radic Res. 2016 Oct;50(10):1106-1115.
116.Zhang L, Lu J, Qiu L.
Synergistic effects of A-B-C-type amphiphilic copolymer on reversal of drug resistance in MCF-7/ADR breast carcinoma.
Int J Nanomedicine. 2016 Oct 11;11:5205-5220.
117.Lu Y, Liu S, Wang Y, Wang D, Gao J, Zhu L.
Asiatic acid uncouples respiration in isolated mouse liver mitochondria and induces HepG2 cells death.
Eur J Pharmacol. 2016 Sep 5;786:212-23.
118.Sun Z, Zhang W, Xue X, Zhang Y, Niu R, Li X, Li B, Wang X, Wang J.
Fluoride decreased the sperm ATP of mice through inhabiting mitochondrial respiration.
Chemosphere. 2016 Feb;144:1012-7.
119.Wang S, Pan J.
Irisin ameliorates depressive-like behaviors in rats by regulating energy metabolism.
Biochem Biophys Res Commun. 2016 May 20;474(1):22-8.
120.Lei L, Zhu Y, Gao W, Du X, Zhang M, Peng Z, Fu S, Li X, Zhe W, Li X, Liu G.
Alpha-lipoic acid attenuates endoplasmic reticulum stress-induced insulin resistance by improvingmitochondrial function in HepG2 cells.
Cell Signal. 2016 Oct;28(10):1441-50.
121.Yang D, Chen H, Zeng X, Xie P, Wang X, Liu C.
Macrophage CGI-58 Attenuates Inflammatory Responsiveness via Promotion of PPARγ Signaling.
Cell Physiol Biochem. 2016;38(2):696-713.
122.Guo H, Nan Y, Zhen Y, Zhang Y, Guo L, Yu K, Huang Q, Zhong Y.
miRNA-451 inhibits glioma cell proliferation and invasion by downregulating glucose transporter 1.
Tumour Biol. 2016 Oct;37(10):13751-13761.
123.Tang L, Cong Z, Hao S, Li P, Huang H, Shen Y, Li K, Jing H.
Protective effect of melatonin on the development of abdominal aortic aneurysm in a rat model.
J Surg Res. 2016 Jun 16. pii:S0022-4804(16)30139-1.
124.Shi F, Wang Y, Li Y, Wang X.
Mode of action of leucocin K7 produced by Leuconostoc mesenteroides K7 against Listeria monocytogenesand its potential in milk preservation.
Biotechnol Lett. 2016 Sep;38(9):1551-7.
125.Yu J, Guo X, Chen R, Feng L.
Downregulation of Mitofusin 2 in Placenta Is Related to Preeclampsia.
Biomed Res Int. 2016;2016:6323086.
126.Guo S, Lv L, Shen Y, Hu Z, He Q, Chen X.
A nanoparticulate pre-chemosensitizer for efficacious chemotherapy of multidrug resistant breast cancer.
Sci Rep. 2016 Feb 15;6:21459.
127.Li Y, Wang J, Xing J, Wang Y, Luo Y.
Surface chemistry regulates the sensitivity and tolerability of osteoblasts to various magnitudes of fluidshear stress.
J Biomed Mater Res A. 2016 Dec;104(12):2978-2991.
128.Hong W, Li G, Nie Y, Zou L, Zhang X, Liu S, Li G, Xu H, Zhang CP, Liang S.
Potential Involvement of P2 Receptors inthe Pathological Processes of Hyperthyroidism: A Pilot Study.
Ann Clin Lab Sci. 2016 May;46(3):254-9.
129.Shi C, Zhang X, Sun Y, Yang M, Song K, Zheng Z, Chen Y, Liu X, Jia Z, Dong R, Cui L, Xia X.
Antimicrobial Activity of Ferulic Acid Against Cronobacter sakazakii and Possible Mechanism of Action.
Foodborne Pathog Dis. 2016 Apr;13(4):196-204.
130.He Y, Wang K, Zhao X, Zhang Y, Ma Y, Hu J.
Differential proteome association study of freeze-thaw damage in ram sperm.
Cryobiology. 2016 Feb;72(1):60-8.
131.Shi C, Song K, Zhang X, Sun Y, Sui Y, Chen Y, Jia Z, Sun H, Sun Z, Xia X.
Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii.
PLoS One. 2016 Jul 14;11(7):e0159006.
132.Lu H, Qian S, Muhammad U, Jiang X, Han J, Lu Z.
Effect of fructose on promoting fengycin biosynthesis in Bacillus amyloliquefaciens fmb-60.
J Appl Microbiol. 2016 Dec;121(6):1653-1664.
133.Wu H, Li Z, Wang Y, Yang P, Li Z, Li H, Wu C.
MiR-106b-mediated Mfn2 suppression is critical for PKM2 induced mitochondrial fusion.
Am J Cancer Res. 2016 Oct 1;6(10):2221-2234.
134.Li CG, Cui WY, Wang H.
Sensitivity of KATP channels to cellular metabolic disorders and the underlying structural basis.
Acta Pharmacol Sin. 2016 Jan;37(1):134-42.
135.Sun Y, Guo W, Wang F, Peng F, Yang Y, Dai X, Liu X, Bai Z.
Transcriptome and Multivariable Data Analysis of Corynebacterium glutamicum under Different DissolvedOxygen Conditions in Bioreactors.
PLoS One. 2016 Dec 1;11(12):e0167156.
136.Huang S, Wang J, Wang L.
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