碧云天多种生物素快速标记试剂盒的比较如下
Cat. No. P0632 P0669 P0663 P0665 P0667
Biotin type Biotin-LC-NHS Biotin-NHS Biotin-SS-NHS-Sulfo Biotin-LC-NHS-Sulfo Biotin-NHS-Sulfo
Molecular formula C20H30N4O6S C14H19N3O5S C19H27N4NaO9S4 C20H29N4NaO9S2 C14H18N3NaO8S2
Molecular weight ~455 ~341 ~607 ~557 ~443
Spacer arm length ~22.4Å ~13.5Å ~24.3Å ~22.4Å ~13.5Å
Mass added to target ~339.5Da ~226.3Da ~389.6Da ~339.5Da ~226.3Da
Cell membrane permeability Cell membrane permeable Cell membrane impermeable
Solubility DMSO, DMF DMSO, DMF, Water
Storage conditions -20ºC, protect from moisture
Reactive groups N-hydroxysuccinimide ester (NHS), reacts with primary amines at pH 7.0-9.0