Biotin is a natural small molecule that often does not interfere with the biological function of macromolecules. It is widely used in the biotin-(strept)avidin system and is also an important cofactor for carboxylases in metabolic pathways [1-2]. The binding of biotin to avidin or streptavidin helps connect target molecules (antibodies, nucleic acids, protein A, etc.) to labeling systems (enzymes, fluorescent probes, etc.). This complex can be used in assays such as immunoprecipitation, immunohistochemistry, flow cytometry, Southern and Northern blot, etc. This method is also suitable for the purification and characterization of target molecules.
Beyotime's newly released Biotinylated Protein Pull-Down Kit (Agarose Resin) (P0654) and Biotinylated Protein Pull-Down Kit (Magnetic Beads) (P0658) provides a novel and efficient way to bind biotinylated proteins, antibodies or other active biomolecules of interest to streptavidin agarose resin or magnetic beads, and then use them to capture the target protein or other binding molecules. This approach is widely used in the study of protein interactions, signaling pathways, and disease mechanisms.
Applications
- Capturing target proteins or other molecules interacting with the biotin-labeled protein, antibody, or other active biomolecules of interest;
- Pull-down and elution of biotin-labeled protein complexes;
- Pull-down and elution of biotinylated protein complexes under acidic conditions;
- To study the interactions between known proteins (also known as bait) and target proteins (also known as prey), or between known proteins and DNA, RNA, or other molecules, as well as to confirm interactions between known proteins and other biomolecules.
Working Principle
Figure 1. The workflow of using Beyotime Biotinylated Protein Pull-Down Kit (Agarose Resin) (P0654) to study the interaction between in vitro labeled biotinylated proteins and target proteins.
Figure 2. The workflow of using Beyotime Biotinylated Protein Pull-Down Kit (Magnetic Beads) (P0658) to study protein-protein interaction.
Key Features
- Fast binding and easy operation: The provided streptavidin agarose resin or magnetic beads quickly bind to biotin-labeled biomolecules with high capacity. All necessary reagents for binding, washing, and eluting biotin-labeled molecules and their interacting proteins or other targets are included, ensuring a simple workflow.
- High specificity and efficient elution.
- Versatile application: Suitable for studying interactions between known proteins and target proteins, or between known proteins and molecules such as DNA or RNA.
Product Information
| Cat. No. | Product Name | Pack Size | Price |
| E6673 | BeyoShaker™ Digital See-Saw Rocking Shaker | 1set | |
| E6800 | BeyoVortex™ Basic Rotation Mixer | 1set | |
| P0632S | Biotin Labeling Kit (Biotin-LC-NHS)) | 5T | |
| P0635S | Desthiobiotinylation and Pull-Down Kit (Agarose Resin) | 5T | |
| P0637S | Desthiobiotinylation and Pull-Down Kit (Magnetic Beads) | 5T | |
| P0654S | Biotinylated Protein Pull-Down Kit (Agarose Resin) | 25T | |
| P0658S | Biotinylated Protein Pull-Down Kit (Magnetic Beads) | 25T | |
| ST661-500ml | TBS (10X) | 500ml | |
| ST663-500ml | TBS (20X) | 500ml | |
| P0013 | Cell Lysis Buffer for Western or IP | 100ml | |
| P0013B | RIPA Lysis Buffer (Strong) | 100ml | |
| P0013C | RIPA Lysis Buffer (Medium) | 100ml | |
| P0013D | RIPA Lysis Buffer (Medium) | 100ml |
References
1. Wilćhek M, Bayer EA. Methods Enzymol. 1990. 184:467-9.
2. Waqas B, Wu A, Yim E, Lipner SR. J Dermatolog Treat. 2022. 33(1):573-574.