Beyotime's Universal Blocking and Antibody Dilution Buffer series (P0268/P0269/P0270/P0271)can be used for Western blot (WB), Immunofluorescence (IF), and Immunohistochemistry (IHC) assays. It handles both blocking and antibody dilution—no need to prepare different reagents for different assays. One bottle does it all!
1. Standard Type
Universal Blocking and Antibody Dilution Buffer (P0268) is the basic version for blocking and antibody dilution in WB, IF, and IHC assays.
2. Quick Type
QuickBlock™ Universal Blocking and Antibody Dilution Buffer (P0269) provides rapid blocking in just 5-15 minutes. This serum- and albumin-free formula delivers low background and high signal-to-noise ratios after antibody incubation.
3. Protein-Free Type
QuickBlock™ Universal Protein-Free Blocking and Antibody Dilution Buffer (P0270) is free of animal-derived substances and proteins, minimizing potential interactions between the sample and foreign proteins. It's perfect for WB, IF, and IHC with very low background and high signal-to-noise ratios, and particularly suitable for detecting protein modifications like phosphorylation and glycosylation, as well as biotin detection.
4. High-Sensitivity Type
SignalUp™ Universal Blocking and Antibody Dilution Buffer (P0271) enhances WB sensitivity by several folds, ensuring specific antigen-antibody binding. It’s ideal for overcoming common issues like low sensitivity and high background in WB, IF, and IHC assays.
Selection Guide
| Product Name | Universal Blocking and Antibody Dilution Buffer | QuickBlock™ Universal Blocking and Antibody Dilution Buffer | QuickBlock™ Universal Protein-Free Blocking and Antibody Dilution Buffer | SignalUp™ Universal Blocking and Antibody Dilution Buffer |
| Cat. No. | P0268 | P0269 | P0270 | P0271 |
| Components | Optimized BSA, detergents, antibody stabilizers, etc. | Special QuickBlock™ components, detergents, antibody stabilizers, etc. Serum- and albumin-free. | Detergents, antibody stabilizers, etc. No protein. | Macromolecules, blocking agents, detergents, etc. |
| Compatibility | Compatible with horseradish peroxidase, alkaline phosphatase, biotin and fluorescence labeled secondary antibodies | Compatible with horseradish peroxidase, alkaline phosphatase, biotin and fluorescence labeled secondary antibodies | Compatible with horseradish peroxidase, alkaline phosphatase, biotin and fluorescence labeled secondary antibodies | Compatible with horseradish peroxidase, alkaline phosphatase, biotin and fluorescence labeled secondary antibodies |
| Blocking Time | 60 min | ~10 min | ~10 min | 60 min |
| Background | Extremely low | Extremely low | Extremely low | Extremely low |
| Features | Minimizing nonspecific antibody binding and enhancing the stability of diluted antibodies. | Biotin-free, preventing interference in biotin-based assays; serum and albumin-free, ensuring a high signal-to-noise ratio. | Protein-free, minimizing microbial growth and enabling long-term stable storage at room temperature; preventing protein-protein interactions, improving accuracy. | Increasing sensitivity in WB, IF, and IHC assays by several to over ten times. |
| Applications | Routine WB, IF, IHC assays | Convenient and efficient WB, IF, IHC | Convenient assays for WB, IF, IHC, protein modifications such as phosphorylation, glycosylation, and biotin-labeling | Highly sensitive WB, IF, IHC, and other immunoassays |
| Stability | ★★★★ | ★★★★ | ★★★★★ | ★★★★ |
| Convenience | ★★★★ | ★★★★★ | ★★★★★ | ★★★★ |
| Blocking effect | ★★★★★ | ★★★★★ | ★★★★ | ★★★★ |
| Sensitivity | ★★★★ | ★★★★ | ★★★★ | ★★★★★ |
| Recommendation Index | ★★★★ | ★★★★★ | ★★★★ | ★★★★ |
Product Information