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pCMV-C-Flag
Cat. No.:
Pack size: 100μg 1μg
Price: RMB
Purpose
Express target protein with C-terminal Flag tag in mammalian cell
Depositor
Beyotime
Publication
Chen et al. Cell Death Dis. 2016 Dec 8;7(12):e2512.
Sequence Information
Brief Information
Name Catalog No. Size Description
pCMV-C-Flag D2632-1μg 4315bp DNA solution
Growth In Bacteria
Bacterial Resistance|Kanamycin
Growth Temperature|37℃
Growth Strain|DH5-Alpha
Copy Number|High Copy
Insert Gene Information
Insert gene|N/A
Species|Synthetic
Insert size (bp)|N/A
Gene Sequences|
Promoter|CMV
T-terminal Tag|N/A
C-terminal Tag|Flag
Cloning Method|Restriction Enzyme
5'→3' Cloning Site|Xma Ⅰ/XbaⅠ
5′ sequencing primer|T3 5'-AATTAACCCTCACTAAAGGG-3'
3′ sequencing primer | T7 5'-GTAATACGACTCACTATAGGGC- 3'
Backbone
Vector backbone|pCMV
Vector type | Mammalian expresstion
Total vector size| 4315
 
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