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pET-N-GST-Thrombin-C-His
Cat. No.:
Pack size: 100μg 1μg
Price: RMB
Purpose
Prokaryotic expression of target protein with N-terminal GST tag(contain thrombin site) and/or N-terminal His tag
Depositor
Beyotime
Publication
Sequence Information
Brief Information
Name Catalog No. Size Description
pET-N-GST-Thrombin-C-His D2911-1μg 5955bp DNA solution
Growth In Bacteria
Bacterial Resistance|Kanamycin
Growth Temperature|37℃
Growth Strain|DH5-Alpha
Copy Number|Low Copy
Insert Gene Information
Insert gene|N/A
Species|Synthetic
Insert size (bp)|N/A
Gene Sequences|
Promoter|T7
T-terminal Tag|GST
C-terminal Tag|6X His
Cloning Method|Restriction Enzyme
5'→3' Cloning Site|BamHⅢ/XhoⅠ
5′ sequencing primer|T7 primer
3′ sequencing primer | pET-R primer 5′-TGCTAGTTATTGCTCAGCGG-3′
Backbone
Vector backbone|pET
Vector type | Prokaryotic
Total vector size| 5955
 
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