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pET-Dual-N-GST-PreScission
Cat. No.:
Pack size: 100μg 1μg
Price: RMB
Purpose
Express two target proteins with N-terminal GST tag(contain PreScission site) for one of them in E. coli
Depositor
Beyotime
Publication
Sequence Information
Brief Information
Name Catalog No. Size Description
pET-Dual-N-GST-PreScission D2933-1μg 6065bp DNA solution
Growth In Bacteria
Bacterial Resistance|Ampicillin
Growth Temperature|37℃
Growth Strain|DH5-Alpha
Copy Number|Low Copy
Insert Gene Information
Insert gene|N/A
Species|Synthetic
Insert size (bp)|N/A
Gene Sequences|
Promoter|T7
T-terminal Tag|GST
C-terminal Tag|N/A
Cloning Method|Restriction Enzyme
5'→3' Cloning Site|BamHⅠ/NotⅠ,NdeⅠ/XhoⅠ
5′ sequencing primer|MCS1-N Primer 5′-GCTATCCCACAAATTGATAAGT-3′; MCS2-N Primer 5′-TTGTACACGGCCGCATAATC-3′
3′ sequencing primer | MCS1-C Primer 5′-GATTATGCGGCCGTGTACAA-3′
Backbone
Vector backbone|pET
Vector type | Prokaryotic
Total vector size| 6065
 
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