碧云天生物技术-Bcl-2抗体(兔多抗)(AB112)

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Bcl-2抗体(兔多抗)

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产品编号: AB112

产品包装:>20次

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价格: ¥ 793.00

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产品编号	产品名称	产品包装	产品价格
AB112	Bcl-2抗体(兔多抗)	>20次	793.00元

来源	用途	交叉反应性	抗体识别位点	Bcl-2分子量
Rabbit	WB, IF, IHC, ICC, FC	H, M	Bcl-2 C-terminal	～28kD

WB, Western blot; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry.
H, Human; M, Mouse.
本Bcl-2抗体(Bcl-2 antibody)为进口分装，用人工合成的Bcl-2羧基端(carboxy-terminal)的一段多肽进行适当修饰后免疫rabbit，然后用protein A和抗原多肽亲和柱经过两步纯化而得到的高纯度抗体。
本Bcl-2抗体可以识别内源性水平的Bcl-2，在生理水平检测时未发现和其它Bcl-2家族蛋白有交叉反应。
Bcl-2是一种抗凋亡蛋白，可以抑制很多凋亡诱导剂诱导的细胞色素c释放(cytochrome c release)，及后续的细胞凋亡。Bcl-2被推测可以影响线粒体的钙离子平衡(calcium homeostasis)和质子流(proton flux)。通常Bcl-2蛋白水平的上调被认为细胞的抗凋亡能力上调，而Bcl-2蛋白水平的显著下降则可能导致细胞凋亡。
配套提供了Western一抗稀释液，可以用于Western检测时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB	IP	IF	IHC	ICC	FC	ELISA	ChIP
1:1000	-	1:100	1:250	1:100	1:150	-	-

本抗体如果用于常规的Western检测至少可以检测20次。
包装清单：

产品编号	产品名称	包装
AB112-1	Bcl-2抗体(兔多抗)	20μl
AB112-2	Western一抗稀释液	20ml
—	说明书	1份

保存条件：
Bcl-2抗体-20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. Western检测：
A. 按照1:1000用碧云天提供的Western一抗稀释液稀释抗体。
B. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
C. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
D. 回收稀释的一抗，4℃保存以备下次继续使用。
E. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。

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