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抗体> 常用一抗> 内参抗体
GAPDH Mouse Monoclonal Antibody
产品编号: AF0006
产品包装:100μl
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价格:¥443.00元
产品简介
使用说明
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产品编号 产品名称 产品包装 产品价格
AF0006 GAPDH Mouse Monoclonal Antibody 100μl 443.00元
来源 用途 交叉反应性 分子量
Mouse WB, ICC, IF H, M, R, C, Dg, Gp,Hm, Mk, Pg, Rb, S,Yeast 36KDa

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected. 
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ELISA ChIP
1:1000 - 1:100 1:50 1:50 - - -

抗体详细信息如下:

About this Antibody

Name

GAPDH Mouse Monoclonal Antibody

Category

Monoclonal antibody (mAb); Primary antibody

Isotype

IgG1

Purification

Affinity purification

About the Immunogen

Immunogen

synthetic peptide (KLH-coupled)

Gene ID

2597/14433/24383

SwissProt

P04406/P16858/P04797

Synonyms

GAPDH; G3PD; GAPD; MGC88685

Category

Metabolism

Background

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

包装清单:

产品编号 产品名称 包装
AF0006 GAPDH Mouse Monoclonal Antibody 100μl
AZ100 Western一抗稀释液 100ml
说明书 1份

保存条件:
GAPDH Mouse Monoclonal Antibody-20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。 
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。 
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
请根据抗体的实际用途选择相应的使用方法。
1. Western检测:

a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋
白膜。
c. 回收稀释的一抗,4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页: http://www.beyotime.com/suppport/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片:


Fig. 1. Western blot analysis on HeLa (1) and NIH/3T3 (2) cell lysates using GAPDHmouse monoclonal antibody (AF0006).

Fig. 2. ICC staining GAPDH in D3 cells (red). Cells were fixed in paraformaldehyde,permeabilized with PBS containing 0.25% Triton X-100 and counterstained with DAPIto visualize the nucleus (blue).

Fig. 3. ICC staining GAPDH in A431 cells (red). Cells were fixed inparaformaldehyde, permeabilized with PBS containing 0.25% Triton X-100 andcounterstained with DAPI to visualize the nucleus (blue).


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