中文 | English
订货电话
400-1683301
800-8283301
电话订货时间:
工作日9:00-17:00
订货邮件
order@beyotime.com
订货QQ
4001683301
微信在线咨询
订单下载
碧云天订单.xls
细胞相关> 细胞死亡与自噬> 细胞凋亡
细胞凋亡与坏死检测试剂盒
产品编号: C1056
产品包装:100次
选择包装
价格: ¥ 246.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
C1056 细胞凋亡与坏死检测试剂盒 100次 246.00元

碧云天生产的细胞凋亡与坏死检测试剂盒(Apoptosis and Necrosis Assay Kit)为您提供了一种经典而又快速简便的细胞凋亡与细胞坏死检测方法。
本试剂盒采用Hoechst 33342和碘化丙啶(Propidium Iodide,PI)双染的方法。细胞发生凋亡时,染色质会固缩。Hoechst 33342可以穿透细胞膜,染色后凋亡细胞荧光会比正常细胞明显增强。碘化丙啶(PI)不能穿透细胞膜,对于具有完整细胞膜的正常细胞或凋亡细胞不能染色。而对于坏死细胞,其细胞膜的完整性丧失,碘化丙啶(PI)可以染色坏死细胞。上述两种染料双染后,使用流式细胞仪或荧光显微镜检测时,正常细胞为弱红色荧光+弱蓝色荧光,凋亡细胞为弱红色荧光+强蓝色荧光,坏死细胞为强红色荧光+强蓝色荧光。参考下图,左图为诱导凋亡前的正常细胞,右图为诱导凋亡后的细胞。

染色快速方便,两种染料的染色仅需20-30分钟,一步染色即可完成。
使用方便,使用流式细胞仪检测时,无需稀释等配制过程,也无需再准备其它任何溶液。
本试剂盒足够检测100个样品,每个样品的细胞数量可以为10-100万。
包装清单:

产品编号 产品名称 包装
C1056-1 细胞染色缓冲液 100ml
C1056-2 Hoechst染色液 0.5ml
C1056-3 PI染色液 0.5ml
说明书 1份

保存条件:
4℃保存六个月有效,-20℃保存一年有效。Hoechst染色液和PI染色液需避光保存。
注意事项:
需使用流式细胞仪进行红色和蓝色双荧光检测。也可使用荧光显微镜检测。
染色后宜尽快检测。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 每个样品收集约10-100万细胞于1.5ml离心管内,离心弃上清。细胞沉淀用0.8-1毫升细胞染色缓冲液重悬。
2. 加入5微升Hoechst染色液。
3. 加入5微升PI染色液。
4. 混匀,冰浴或4℃孵育20-30分钟。
5. 用流式细胞仪检测红色荧光和蓝色荧光。
6. 如果使用荧光显微镜检测,检测前离心沉淀细胞,用PBS洗涤一次,再涂片观察红色荧光和蓝色荧光。对于贴壁细胞使用荧光显微镜检测,可以不收集细胞,直接依次按照上述比例加入细胞染色缓冲液、Hoechst染色液和PI染色液冰浴或4℃染色20-30分钟。染色后PBS洗涤一次,再在荧光显微镜下观察。
物质安全数据单
输入右侧验证码点击下载:
更换校验码
质检证书
输入批号搜寻COA
搜索
相关产品请点击如下按钮:
使用本产品的文献:
1. Yunmao Zhang, Yuhang Han, Xiaoliang Ji, Duyang Zang, Long Qiao, Zhizhi Sheng, Chunyan Wang, Shuli Wang, Miao Wang, Yaqi Hou, Xinyu Chen, Xu Hou
.
Nature. 2022 Oct;610(7930):74-80. doi: 10.1038/s41586-022-05124-y. (IF 42.778)
2. Jin S, Zhang QY, Kang XM, Wang JX, Zhao WH.
.
Ann Oncol. 2010;21(2):263-8. (IF 18.274)
3. Dai J, Li Y, Long Z, Jiang R, Zhuang Z, Wang Z, Zhao Z, Lou X, Xia F, Tang BZ
.
ACS Nano. 2020 Jan 28 14(1):854-866. (IF 14.588)
4. Yi P, Chen G, Zhang H, Tian F, Tan B, Dai J, Wang Q, Deng Z.
.
Biomaterials. 2013 Apr;34(12):3010-9. (IF 10.317)
5. Li C, Du L, Ren Y, Liu X, Jiao Q, Cui D, Wen M, Wang C, Wei G, Wang Y, Ji A, Wang Q
.
J EXP CLIN CANC RES. 2019 Feb 13 38(1):76. (IF 7.068)
6. Xiaolei Hu, Ling Xu, Xuewei Fu, Jiao Huang, Ping Ji, Zhiwei Zhang, Feng Deng, Xiaomian Wu
.
Nanoscale. 2021 May 20;13(19):8976-8990. doi: 10.1039/d1nr00696g. (IF 6.895)
7. Zhou ZF, Sun TW, Qin YH, Zhu YJ, Jiang YY, Zhang Y, Liu JJ, Wu J, He SS, Chen F
.
BIOMATER SCI-UK. 2019 Nov 19 7(12):5044-5053. (IF 6.183)
8. Zhang J, Ahmad S, Wang LY, Han Q, Zhang JC, Luo YP
.
FREE RADICAL BIO MED. 2019 Jun 137:87-98. (IF 6.17)
9. Huang X, Yi C, Fan Y, Zhang Y, Zhao L, Liang Z, Pan J.
.
MAT SCI ENG C-MATER. 2014 Sep 1;42:325-32. (IF 5.88)
10. Huang G, Dong JY, Zhang QJ, Meng QQ, Zhao HR, Zhu BQ, Li SS
.
Eur J Med Chem. 2019 Mar 1 165:160-171. (IF 5.572)
12. He W, Huang L, Li M, Yang Y, Chen Z, Shen X.
.
CELL PHYSIOL BIOCHEM. 2017;44(2):792-803. (IF 5.5)
13. Ma L, Wang H, Wang C, Su J, Xie Q, Xu L, Yu Y, Liu S, Li S, Xu Y, Li Z.
.
Aging Dis. 2016 May 27;7(3):254-66. (IF 5.402)
14. Li G, Ye L, Pan J, Long M, Zhao Z, Yang H, Tian J, Wen Y, Dong S, Guan J, Luo B.
.
Liver Int. 2012 Jul;32(6):998-1007. (IF 5.175)
15. Wang J, Qi L, Mei L, Wu Z, Wang H C.
.
Int J Biol Macromol. 2016 Jul;88:81-7. (IF 5.162)
16. Du X, Zhang T, Ma G, Gu X, Wang G, Li J
.
INT J NANOMED. 2019 Apr 2 14:2233-2251. (IF 5.115)
17. Chang L, Wang Z, Ma F, Tran B, Zhong R, Xiong Y, Dai T, Wu J, Xin X, Guo W, Xie Y, Mao Y, Zhu YZ
.
Oxid Med Cell Longev. 2019 Jun 2 2019:6173685. (IF 5.076)
18. Banerjee J, Ghose J, Sinha M, Sen S.
.
Oxid Med Cell Longev. 2019 Jul 22 2019:3764252. (IF 5.076)
19. Jing Wang, Wei Zhang, Sixin Wang, Yamin Wang, Xu Chu, Haifeng Ji
.
Oxid Med Cell Longev. 2021 Jul 30;2021:8936907. doi: 10.1155/2021/8936907. (IF 5.076)
20. Gu-Hua Tang, Jing-Hong Liu, Xin-Yuan Sun, Jian-Ming Ouyang
.
Oxid Med Cell Longev. 2022 Aug 12:2022:2082263. doi: 10.1155/2022/2082263. (IF 5.076)
21. Ma W, Xu W, Xu H, Chen Y, He Z, Ma M.
.
Planta. 2010 Jul;232(2):325-35. (IF 3.39)
22. Song J, Guo X, Xie X, Zhao X, Li D, Deng W, Song Y, Shen F, Wu M, Wei L.
.
J Cell Biochem. 2011 Nov;112(11):3406-20. (IF 4.237)
23. Meng Q, Lv J, Ge H, Zhang L, Xue F, Zhu Y, Liu P.
.
Mol Biol Rep. 2012 May;39(5):5867-73. (IF 1.402)
24. Tang S, Hu J, Meng Q, Dong X, Wang K, Qi Y, Chu C, Zhang X, Hou L.
.
Cell Biochem Biophys. 2013 Mar;65(2):197-202. (IF 2.073)
25. Wang B, Yu T, Dong X, Zhang Z, Song L, Xu Y, Zhang XH.
.
Vet Microbiol. 2013 May 3;163(3-4):282-9. (IF 3.03)
26. Yin X, Zhou X, Xue Z, Tian L, Zhou Y, Yang L, Zhao D.
.
Mol Cell Biochem. 2014 Jan;385(1-2):43-52. (IF 2.795)
27. Cai XY, Xiong LM, Yang SH, Shao ZW, Xie M, Gao F, Ding F.
.
Spine J. 2014 Mar 1;14(3):483-90. (IF 3.191)
28. Zhu W, Yang B, Fu H, Ma L, Liu T, Chai R, Zheng Z, Zhang Q, Li G.
.
BIOCHEM BIOPH RES CO. 2015 Mar 13;458(3):590-5. (IF 2.985)
29. Han Y, Shao Y, Liu T, Qu YL, Li W, Liu Z.
.
PLoS One. 2015 Apr 8;10(4):e0122951. (IF 2.74)
30. Chen F, Li Q, Zhang Z, Lin P, Lei L, Wang A, Jin Y.
.
Int J Mol Sci. 2015 Aug 20;16(8):19780-95. (IF 4.556)
31. Sun Y, Zhang J, Zhou J, Huang Z, Hu H, Qiao M, Zhao X, Chen D.
.
Eur J Pharmacol. 2015 Dec 5;768:28-40. (IF 3.263)
33. Chen F, Lin P, Wang N, Yang D, Wen X, Zhou D, Wang A, Jin Y.
.
Toxicol In Vitro. 2016 Apr;32:115-22. (IF 2.959)
35. Wang Y, Wu Y, Wang Y, Fu A, Gong L, Li W, Li Y.
.
APPL MICROBIOL BIOT. 2016 Dec 12. [Epub ahead of print] (IF 3.53)
36. Wang Y, Wang X, Shi J, Zhu R, Zhang J, Zhang Z, Ma D, Hou Y, Lin F, Yang J.
.
SCI REP-UK. 2016 Dec 20;6:39477. (IF 3.998)
37. Yi T, Wu X, Long Z, Duan G, Wu Z, Li H, Chen H, Zhou X.
.
Biomed Res Int. 2017;2017:1314297. (IF 2.276)
38. Jin X, Yu ZF, Chen F, Lu GX, Ding XY, Xie LJ, Sun JT.
.
Front Cell Neurosci. 2017 Mar 7;11:66. (IF 3.921)
39. Wang Y, Wu Y, Wang Y, Fu A, Gong L, Li W, Li Y.
.
APPL MICROBIOL BIOT. 2017 Apr;101(7):3015-3026. (IF 3.53)
40. Song C, Luo B, Gong L.
.
PLoS One. 2017 Apr 13;12(4):e0175009. (IF 2.74)
42. Long Z, Duan G, Li H, Yi T, Wu X, Chen F, Wu Z, Gao Y.
.
PLoS One. 2017 Jul 27;12(7):e0181903. (IF 2.74)
43. Pang J, Zhou Q, Sun X, Li L, Zhou B, Zeng F, Zhao Y, Shen W, Sun Z.
.
TOXICOL APPL PHARM . 2017 Oct 15;333:60-67. (IF 3.616)
44. Sun Q, Wang J, Li Y, Zhuang J, Zhang Q, Sun X, Sun D.
.
Chem Biol Drug Des. 2017 Nov;90(5):1019-1028. (IF 2.548)
45. Du J, Zhang P, Gan M, Zhao X, Xu Y, Li Q, Jiang Y, Tang G, Li M, Wang J, Li X, Zhang S, Zhu L
.
Gene. 2018 Aug 20;668:1-7. (IF 2.984)
46. Ma J, Zhang J, Wang Y, Long K, Wang X, Jin L, Tang Q, Zhu L, Tang G, Li X, Li M
.
Gene. 2018 Oct 30;675:36-43. (IF 2.984)
47. Zhu K, Li K, Wang H, Kang L, Dang C, Zhang Y
.
Pharmacology. 2019 104(3-4):113-125. (IF 1.625)
48. Le L, Fu H, Lv Q, Bai X, Zhao Y, Xiang J, Jiang B, Hu K, Chen S
.
Phytomedicine. 2019 Feb 53:193-204. (IF 4.268)
49. Yang X, Zhang Y, Lai W, Xiang Z, Tu B, Li D, Nan X, Chen C, Hu Z, Fang Q
.
Nanotoxicology. 2019 Feb 13(1):35-49. (IF 4.925)
50. Wu J, Liu H, Chu T, Jiang P, Li ST
.
J Muscle Res Cell Motil. 2019 Mar 40(1):43-51.
51. Li W, Yue X, Li F
.
ASIAN AUSTRAL J ANIM. 2019 May 32(5):629-636. (IF 1.664)
52. Zhang J, Qiu W, Ma J, Wang Y, Hu Z, Long K, Wang X, Jin L, Tang Q, Tang G, Zhu L, Li X, Shuai S, Li M
.
Int J Mol Sci. 2019 May 16 20(10). pii: E2418. (IF 4.556)
53. Wang JR, Luo YH, Piao XJ, Zhang Y, Feng YC, Li JQ, Xu WT, Zhang Y, Zhang T, Wang SN, Xue H, Wang WZ, Cao LK, Jin CH
.
Drug Dev Res. 2019 Jun 80(4):461-470.
54. He C, Zhao Y, Jiang X, Liang X, Yin L, Yin Z, Geng Y, Zhong Z, Song X, Zou Y, Li L, Zhang W, Lv C
.
Int Immunopharmacol. 2019 Jun 71:328-335. (IF 3.943)
55. Wang JR, Shen GN, Luo YH, Piao XJ, Zhang Y, Wang H, Li JQ, Xu WT, Zhang Y, Wang SN, Zhang T, Xue H, Cao LK, Jin CH
.
J Chemother. 2019 Jul 31(4):214-226.
56. Yang Q, Bai SY, Li LF, Li S, Zhang Y, Munir M, Qiu HJ
.
J Virol. 2019 Jul 30 93(16). pii: e00718-19. (IF 4.501)
57. Zhou XL, Chen ZD, Zhou YM, Shi RH, Li ZJ
.
Molecules. 2019 Aug 26 24(17). pii: E3092. (IF 3.267)
58. Yang Y, Wang C, Zhuge Y, Zhang J, Xu K, Zhang Q, Zhang H, Chen H, Chu M, Jia C
.
Front Microbiol. 2019 Aug 6 10:1810. (IF 4.235)
59. Wang H, Cheng J, Wang H, Wang M, Zhao J, Wu Z
.
J Food Biochem. 2019 Dec 43(12):e13052. (IF 1.662)
60. Sun N, Liang Y, Chen Y, Wang L, Li D, Liang Z, Sun L, Wang Y, Niu H
.
Int J Mol Med. 2019 Dec 44(6):2189-2200. (IF 3.098)
62. Zhou J, Li G, Han G, Feng S, Liu Y, Chen J, Liu C, Zhao L, Jin F
.
INVEST NEW DRUG. 2020 Feb 38(1):50-59. (IF 3.525)
63. Yiyi Li, Xuan Niu, Haitao Xu, Qingwen Li, Lanxia Meng, Mingyang He, Jie Zhang, Zhentao Zhang, Zhaohui Zhang
.
Exp Cell Res. 2020 Apr 1;389(1):111847. doi: 10.1016/j.yexcr.2020.111847. (IF 3.383)
64. Wen-Hui Xie, Jian Ding, Xiao-Xia Xie, Xiao-Huang Yang, Xiao-Fan Wu, Zhi-Xin Chen, Qi-Lan Guo, Wen-Yu Gao, Xiao-Zhong Wang, Dan Li
.
Inflamm Res. 2020 Jul;69(7):683-696. doi: 10.1007/s00011-020-01351-z. (IF 3.174)
65. Mengjie Wang, Jingwen Tan, Chenglan Jiang, Shuting Li, Xinan Wu, Guanghui Ni, Yuefeng He
.
Environ Geochem Health. 2021 Jan;43(1):475-484. doi: 10.1007/s10653-020-00740-x.
66. Hongchao Gou, Zhibiao Bian, Rujian Cai, Pinpin Chu, Shuai Song, Yan Li, Zhiyong Jiang, Kunli Zhang, Dongxia Yang, Chunling Li
.
Front Microbiol. 2021 Jun 4;12:664353. doi: 10.3389/fmicb.2021.664353. (IF 4.235)
67. Jie Tang, Bo Hu, Huaping Zheng, Xiaolan Qian, Yi Zhang, Jia Zhu, Guangtao Xu, Deqing Chen, Xin Jin, Wanlu Li, Long Xu
.
Ecotoxicol Environ Saf. 2021 Sep 15;221:112423. doi: 10.1016/j.ecoenv.2021.112423.
68. Jingjing Li, Chaowu Yang, Peng Ren, Zhongzhen Lin, Donghao Zhang, Xiaosong Jiang, Li Wang, Yiping Liu
.
Mol Biol Rep. 2021 Dec;48(12):7985-7997. doi: 10.1007/s11033-021-06831-x. (IF 1.402)
69. Wenhuan Guo, Zehua Zeng, Cencan Xing, Jinghui Zhang, Wangyu Bi, Jingjie Yang, Roshan Shah, Donghui Wang, Yingxian Li, Xiaoshuang Zhang, Yongzhong Bian, Hongwu Du
.
Cytotherapy. 2022 Jan;24(1):59-71. doi: 10.1016/j.jcyt.2021.07.018. (IF 4.218)
70. Jing Sun, Yanshu Zhang, Licheng Yan, Si Liu, Weicheng Wang, Yi Zhu, Weixuan Wang, Shuang Li, Bin He, Lei Wu, Lijin Zhang
.
J Inorg Biochem. 2022 Jun:231:111792. doi: 10.1016/j.jinorgbio.2022.111792. (IF 3.212)
71. Yaqiong An, Haibo Li, Mengxiao Wang, Zhaohua Xia, Lexi Ding, Xiaobo Xia
.
Biomed Pharmacother. 2022 Sep:153:113378. doi: 10.1016/j.biopha.2022.113378. (IF 4.545)
72. Ruonan Gao, Zhou Chen, Yubin Wu, Ruiyu Chen, Wenrong Zheng, Liqin Qi, Xiaoying Liu, Xiaohong Liu, Libin Liu
.
Free Radic Biol Med. 2022 Nov 20;193(Pt 1):405-420. doi: 10.1016/j.freeradbiomed.2022.10.313.
73. Shunlian Hu, Lu Wang, Yahan Xu, Fajiu Li, Tao Wang
.
Respir Res. 2022 Dec 16;23(1):353. doi: 10.1186/s12931-022-02279-0.
74. Min Li, Shengguang Wang, Ce Zhang, Chenglin Chi, Rongxia Liu, Tian Wang, Fenghua Fu
.
Int Immunopharmacol. 2023 Feb:115:109659. doi: 10.1016/j.intimp.2022.109659. (IF 3.943)
75. Xiancheng Qiu, Qianke Tao, Lihan Zhang, Chenghao Kuang, Yuke Xie, Lifang Zhang, Shigang Yin, Jianhua Peng, Yong Jiang
.
J Neurochem. 2023 Mar;164(6):829-846. doi: 10.1111/jnc.15751. (IF 4.066)
购物车
会员
顶部