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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| C6228 | DLD-1 (人结直肠腺癌上皮细胞) | 1支/瓶 | 1250.00元 |
| Organism | Tissue | Morphology | Culture Properties |
| Homo sapiens, human | Large intestine; Colon | Epithelial | Adherent |
本细胞株详细信息如下:
| General Information | |
| Cell Line Name | DLD-1 (Human Colorectal Adenocarcinoma Epithelial Cells) |
| Synonyms | DLD 1; DLD1; CoCL3 |
| Organism | Homo sapiens, human |
| Tissue | Large intestine; Colon |
| Cell Type | Colorectal adenocarcinoma |
| Morphology | Epithelial |
| Disease | Adenocarcinoma; Colorectal; Dukes' type C |
| Strain | - |
| Biosafety Level* | 1 |
| Age at Sampling | Adult |
| Gender | male |
| Genetics | - |
| Ethnicity | - |
| Applications | 3D cell culture; Cancer research; a suitable transfection host |
| Category | Cancer cell line |
* Biosafety classification is based onU.S. Public Health Service Guidelines,it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
| Characteristics | |
| Karyotype | This is a pseudodiploid human cell line with the modal chromosome number of 46, occurring in 86% of cells. The rate of polyploidy was high at 17.1%. The karyotype of the line was 46,XY,-2,+dir dup(2)(p13-p23). The Y chromosome was slightly longer than N22 and had a large segment of heterochromatic, fluorescent distal q arms. |
| Virus Susceptibility | - |
| Derivation | DLD-1 is one of two colorectal adenocarcinoma cell lines which were isolated by D.L. Dexter and associates during a period from 1977-1979 A culture of unknown passage submitted to the ATCC in 1979 was found to be contaminated with Mycoplasma hyorhinis. |
| Clinical Data | male |
| Antigen Expression | Blood type O The cells are weakly positive for keratins and vimentin. The cells are positive for keratin by immunoperoxidase staining. DLD-1 cells are positive for p53 antigen expression (the p53 antigen produced has a C -> T mutation resulting in Ser -> Phe at position 241). |
| Receptor Expression | - |
| Oncogene | myc +; myb + ; ras +; fos +; sis +; p53 +; abl -; ros -; src - |
| Genes Expressed | carcinoembryonic antigen (CEA) 0.5 ng/106 cells/10 days; colon antigen 3 |
| Gene expression databases | ArrayExpress; E-MTAB-2706 GEO; GSM206467 GEO; GSM274715 GEO; GSM274716 GEO; GSM274727 GEO; GSM513817 GEO; GSM514291 GEO; GSM741247 GEO; GSM886979 GEO; GSM888048 GEO; GSM1346869 GEO; GSM1374463 GEO; GSM1448161 GEO; GSM1696579 GEO; GSM1696580 GEO; GSM1696581 GEO; GSM1696582 GEO; GSM2549997 |
| Metastasis | - |
| Tumorigenic | Yes |
| Effects | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments | This cell line is one of four colorectal adenocarcinoma cell lines that have been identified as being derived from a single individual. DNA profiling studies have shown that DLD-1 (ATCC CCL-221), HCT-15 (ATCC CCL-225), HCT-8 (ATCC CCL-244) and HRT-18G (ATCC CRL-11663) share a single profile. DNA fingerprinting and cytogenetic analyses performed at ATCC and elsewhere show the line is similar to HCT-15 (CCL-225) and suggest the two are of different clonal origin from the same individual. Their genetic origin has been confirmed by DNA fingerprinting; however, cytogenetic analysis has shown that they lack concurrent marker chromosomes or concurrent numerical changes. A culture of unknown passage submitted to the ATCC in 1979 was found to be contaminated with Mycoplasma hyorhinis. The cells were subsequently cured using a combination of antibiotics over a 12-week cultivation period. Following treatment, the cells were assayed by the Hoechst stain weekly and by the standard culture test periodically. During 11 consecutive months of cultivation in the absence of antibiotics, all of these tests were negative. |
| Culture Method | |
| Doubling Time | ca. 48 hours |
| Methods for Passages | Wash by PBS once then 0.25% trypsin-EDTA solution and incubate at room temperature (or at 37ºC), observe cells under an inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes) |
| Medium | RPMI 1640 + 10% FBS，推荐使用DLD-1细胞完全培养液(含血清等) (C6228C)。 |
| Special Remarks | - |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | 1:3 to 1:10 |
| Growth Condition | air, 95%; carbon dioxide (CO2), 5%; 37°C |
| Freeze medium | RPMI 1640+20% FBS+10% DMSO，也可以订购碧云天的细胞冻存液(C0210)或BeyoAOF™无血清细胞冻存液(C0210B)。 |
本细胞株经过支原体检测(Mycoplasma Test)，检测结果为阴性。
本细胞株通过STR (short tandem repeats)鉴定，具体结果如下：
| AMEL | D16S539 | D7S820 |
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| D5S818 | THO1 | TPOX |
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| vWA | D13S317 | CSF1PO |
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本细胞株的培养图片如下：
| 产品编号 | 产品名称 | 包装 |
| C6228 | DLD-1 (人结直肠腺癌上皮细胞) | 1支/瓶 |
| — | 说明书 | 1份 |
对于细胞培养瓶或离心管运输的活细胞，室温3-5天有效。对于干冰运输的冻存细胞，液氮保存，长期有效；-80℃保存，2个月有效。
注意事项：本细胞株未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明细胞株的来源。
本细胞适合使用胎牛血清培养, 推荐选购碧云天生产的DLD-1细胞培养血清(C6228F)，确保细胞株和血清之间有很好兼容性。该血清经测试可以很好地用于DLD-1细胞的培养，细胞生长速度和生长形态良好。
本细胞的完全培养液可以自行配制，推荐选购碧云天生产的DLD-1细胞完全培养液(含血清等) (C6228C），该培养液已经添加了优质胎牛血清和所需的添加剂如双抗以及细胞培养所必须的添加剂，可以直接使用，让细胞无须驯化就能正常生长。
本细胞株相关资料参考ATCC (American Type Culture Collection)、DSMZ (German Collection of Microorganisms and Cell Cultures)、JCRB (Japanese Collection of Research Bioresources Cell Bank)、Cellosaurus (Swiss Institute of Bioinformatics)等网站信息，并结合碧云天实际培养信息综合而成。由于细胞培养的条件、代数等因素，实际细胞可能与本说明书提供的信息有一定的差异，具体以实际细胞为准。
STR结果可以与ATCC、DSMZ及中国国家实验细胞资源共享平台等网站的数据库进行比对，匹配度80%以上即可认为该细胞系正确。
本产品会根据细胞是否正在培养、目的地距离等因素确定运输方式：冷冻的细胞冻存管(干冰)、一小瓶贴壁培养的细胞或一小瓶/管悬浮培养的细胞(常温)。为了更好地耐受长途运输和环境温度等变化，对于正常贴壁培养的细胞，也可能会以悬浮的形式培养在细胞培养瓶或离心管中进行运输。
对于干冰运输的冻存细胞，若干冰已经完全融化，请立即将细胞复苏培养，切勿再次低温冻存；若尚留有干冰，请直接复苏培养或立即将含有细胞的冻存管放入液氮中保存待用，切不可将细胞置于高温环境。
收到冻存的细胞后请尽快复苏细胞进行培养，以确认细胞活力、状态并保种。如暂时不进行复苏操作，冻存细胞可在-80℃条件下保存2个月。
每支冻存管约含1×106个细胞，体积为0.5-1ml，预期存活率60-90%，建议复苏至1个6cm培养皿中。如果复苏后存活率较低，可以消化后转移至3.5cm培养皿中，这样细胞生长会更好。
如果本产品是常温运输，并且是培养瓶中充满完全培养液的贴壁细胞，收到细胞后请在显微镜下观察细胞生长状态，如果细胞密度超过85%请尽快进行传代操作；如果悬浮的细胞较多，请将培养瓶置于培养箱中静置过夜以使悬浮的细胞再次贴壁。如果收到的是常温运输的离心管装的悬浮细胞，可以直接取出转移至培养皿或培养瓶中培养。若培养液颜色正常则保留培养液继续培养，并且在首次更换培养液时，保留一半原培养液，并加入一半新鲜培养液，这样可以尽量避免由于培养液或血清差异导致细胞生长的不适应，确保细胞良好的生长状态。
细胞培养请在生物安全柜台中进行操作，并严格遵守无菌操作。
请在培养液中加入适量青霉素-链霉素溶液以防止可能的细菌污染，如碧云天的青霉素-链霉素溶液(100X) (C0222)。
理论上永生化细胞可无限传代，但为了保证细胞的良好状态，建议最早培养的几代细胞就冻存一批，并每培养一段时间后复苏早期冻存的细胞进行培养。
接收、处理、保存、丢弃及使用细胞的时候要遵守相关法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| C6228 | DLD-1 (人结直肠腺癌上皮细胞) | 1支/瓶 |
| C6228C | DLD-1细胞完全培养液(含血清等) | 550ml |
| C6228F-50ml/500ml | DLD-1细胞培养血清 | 50ml/500ml |
| C0191-100ml/500ml | BeyoTryp™ Express Enzyme (1X, 含酚红, AOF) | 100ml/500ml |
| C0192-100ml/500ml | BeyoTryp™ Express Enzyme (1X, 不含酚红, AOF) | 100ml/500ml |
| C0193-100ml | BeyoTryp™ Express Enzyme (10X, 含酚红, AOF) | 100ml |
| C0194-100ml | BeyoTryp™ Express Enzyme (10X, 不含酚红, AOF) | 100ml |
| C0196-100ml/500ml | 0.5M EDTA, pH8.0 (Sterile, Cell Culture Grade) | 100ml/500ml |
| C0198-100ml | BeyoAOF™ 0.02% EDTA细胞解离液(Versene溶液) | 100ml |
| C0201-100ml/500ml | 胰酶细胞消化液(0.25%胰酶) | 100ml/500ml |
| C0202 | 胰酶细胞消化液(0.05%胰酶) | 100ml |
| C0203-100ml/500ml | 胰酶细胞消化液(0.25%胰酶, 含酚红) | 100ml/500ml |
| C0204 | 胰酶细胞消化液(0.05%胰酶, 含酚红) | 100ml |
| C0205 | 胰酶细胞消化液(0.25%胰酶, 不含EDTA) | 100ml |
| C0207 | 胰酶细胞消化液(0.25%胰酶, 含酚红, 不含EDTA) | 100ml |
| C0208-100ml/500ml | BeyoAOF™重组胰酶细胞消化液(含酚红) | 100ml/500ml |
| C0209-100ml/500ml | BeyoAOF™重组胰酶细胞消化液(不含酚红) | 100ml/500ml |
| C0210 | 细胞冻存液 | 50ml |
| C0210B-50ml/200ml | BeyoAOF™无血清细胞冻存液 | 50ml/200ml |
| C0211-100ml | L-Ala-Gln (100X) | 100ml |
| C0212 | L-Glutamine (100X) | 100ml |
| C0215-100ml/500ml | HEPES Solution (1M, pH7.3, 细胞培养用) | 100ml/500ml |
| C0217-100ml/500ml | HEPES Solution (1M, pH7.5, 细胞培养用) | 100ml/500ml |
| C0218 | Hanks' Balanced Salt Solution | 500ml |
| C0219 | Hanks' Balanced Salt Solution (with Ca2+ & Mg2+) | 500ml |
| C0220 | 7.5% NaHCO3溶液 | 100ml |
| C0221A | PBS | 500ml |
| C0221D | D-PBS | 500ml |
| C0221G | D-PBS (with Ca2+ & Mg2+) | 500ml |
| C0222 | 青霉素-链霉素溶液(100X) | 100ml |
| C0223-100ml | 青霉素-链霉素-庆大霉素溶液(100X) | 100ml |
| C0224-100ml | 青霉素-链霉素-两性霉素B溶液(100X) | 100ml |
| C0226 | 胎牛血清(Biowest原装, 产地南美) | 500ml |
| C0226S | 胎牛血清(Biowest分装, 产地南美) | 50ml |
| C0229-1L | 新生牛血清(AusgeneX原装，产地新西兰) | 1L |
| C0232 | 胎牛血清(Gibco原装, 产地南美) | 500ml |
| C0234 | 胎牛血清(Gibco分装, 产地澳洲) | 50ml |
| C0235 | 胎牛血清(Gibco原装, 产地澳洲) | 500ml |
| C0237 | 胎牛血清(Gibco原装, 产地澳洲) | 500ml |
| C0238 | 胎牛血清(Gibco原装, 产地澳洲) | 500ml |
| C0251 | 胎牛血清(产地南美) | 50ml |
| C0252 | 胎牛血清(产地南美) | 500ml |
| C0258 | 新生牛血清(产地新西兰) | 50ml |
| C0265 | 山羊血清 | 50ml |
| C0280S/M/L | Myco-Zero™支原体去除试剂 | 5次/20次/100次 |
| C0283-500ml/2L | Myco-Zero™支原体去除喷雾剂 | 500ml/2L |
| C0285S/M/L | Myco-Zero™ Plus支原体去除试剂 | 50次/250次/1000次 |
| C0288S/M | 支原体清除试剂 | 20mg/100mg |
| C0290S/M | 支原体清除试剂Plus | 10mg/50mg |
| C0292-2ml/10ml | 支原体预防去除试剂I | 2ml/10ml |
| C0293-2ml/10ml | 支原体预防去除试剂II | 2ml/10ml |
| C0296 | 支原体染色检测试剂盒 | >100次 |
| C0297S/M | Myco-Lumi™发光法支原体检测试剂盒(低灵敏度仪器用) | 20次/100次 |
| C0298S/M | Myco-Lumi™发光法支原体检测试剂盒(高灵敏度仪器用) | 20次/100次 |
| C0299S | Myco-Lumi™发光法支原体检测阳性对照 | 20次 |
| C0301S | 支原体PCR检测试剂盒 | 250次 |
| C0303S/M | BeyoDirect™支原体qPCR检测试剂盒 | 100次/500次 |
| C0305S/M | BeyoColor™支原体等温扩增变色检测试剂盒 | 25次/100次 |
| FBX081 | 81孔液氮罐专用冻存盒 | 1个/包 |
| FBX082 | 100孔液氮罐专用冻存盒 | 1个/包 |
| FCFC012 | BeyoCool™细胞冻存盒 | 1个/盒 |
| ST857-100ml/500ml | BeyoClean™细胞培养箱用水抑菌剂(500X) | 100ml/500ml |
| ST875-100ml/500ml | BeyoPure™ Ultrapure Water (细胞培养级) | 100ml/500ml |
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