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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| C6606 | MRC-5 (人胚肺细胞) | 1支/瓶 | 1558.00元 |
| Organism | Tissue | Morphology | Culture Properties |
| Homo sapiens (Human) | Lung | Fibroblast | adherent |
本细胞株详细信息如下:
| General Information | |
| Cell Line Name | MRC-5 (Human embryonic lung fibroblast Cells) |
| Synonyms | MRC5; MRC 5; MRCV; MRC-V; Medical Research Council cell strain-5 |
| Organism | Homo sapiens (Human) |
| Tissue | Lung |
| Cell Type | Fibroblast |
| Morphology | fibroblast |
| Disease | Normal |
| Passage | P20-P22 (this is finite line with maximal passage number of 46 |
| Strain | － |
| Biosafety Level* | 1 |
| Age at Sampling | 14 weeks gestation |
| Gender | Male |
| Genetics | － |
| Ethnicity | － |
| Applications | efficacy testing transfection host viruscide testing |
| Category | Finite cell line |
* Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
| Characteristics | |
| Karyotype | Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Virus Susceptibility | Human poliovirus 1 Herpes simplex virus Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana) |
| Derivation | The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male embryo by J.P. Jacobs in September of 1966. |
| Clinical Data | White; Male ; 14-week-old male embryo |
| Antigen Expression | － |
| Receptor Expression | － |
| Oncogene | － |
| Genes Expressed | － |
| Gene expression databases | GEO: GSM248209; GSM248210; GSM520665; GSM520666; GSM520667; GSM579888; GSM579889; GSM607969; GSM3738752; GSM3738753 |
| Metastasis | － |
| Tumorigenic | － |
| Effects | － |
| Comments | The cells are capable of 42 to 46 population doublings before the onset of senescence. |
| Culture Method | |
| Doubling Time | ~34 hours |
| Methods for Passages | Wash by PBS once then 0.25% (w/v) Trypsin-0.53 mM EDTA solution and incubate at room temperature (or at 37℃), observe cells under an inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes) |
| Medium | MEM +10% FBS+1mM Sodium Pyruvate+2mM L-Alanyl-L-Glutamine + 1 mM non-essential amino acids + 100 U/ml penicillin and 1 mg/ml of a streptomycin |
| Special Remarks | 1. The cells were sensitive to DMSO, so they should be centrifuged to remove the refrigerant before being cultured. 2. The cells were a limited cell line and were passaged to P42-P46 at most (depending on the culture method and serum quality), after which the cells proliferated slowly. |
| Medium Renewal | 1 to 2 times per week |
| Subcultivation Ratio | 1:2 to 1:5 |
| Growth Condition | 95% air+ 5% CO2, 37℃ |
| Freeze medium | Complete growth medium supplemented with 5% (v/v) DMSO，也可以订购碧云天的细胞无血清冻存液(C0210B)。 |
本细胞株经过支原体检测(Mycoplasma Test)，检测结果为阴性。
本细胞株通过STR (short tandem repeats)鉴定，具体结果如下：
| Amelogenin | CSF1PO | D13S317 | D16S539 | D5S818 | D7S820 | THO1 | TPOX | vWA |
| X,Y | 11,12 | 11,14 | 9,11 | 11,12 | 10,11 | 8 | 6,8 | 15 |
本细胞株的培养图片如下：
| 产品编号 | 产品名称 | 包装 |
| C6606 | MRC-5 (人胚肺细胞) | 1支/瓶 |
| － | 说明书 | 1份 |
对于细胞培养瓶或离心管运输的活细胞，室温3-5天有效。对于干冰运输的冻存细胞，液氮保存，长期有效；-80℃保存，2个月有效。
注意事项：本细胞株未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明细胞株的来源。
本细胞株相关资料参考ATCC (American Type Culture Collection)、DSMZ (German Collection of Microorganisms and Cell Cultures)、JCRB (Japanese Collection of Research Bioresources Cell Bank)、Cellosaurus (Swiss Institute of Bioinformatics)等网站信息，并结合碧云天实际培养信息综合而成。由于细胞培养的条件、代数等因素，实际细胞可能与本说明书提供的信息有一定的差异，具体以实际细胞为准。
STR结果可以与ATCC、DSMZ及中国国家实验细胞资源共享平台等网站的数据库进行比对，匹配度80%以上即可认为该细胞系正确。
本产品会根据细胞是否正在培养、目的地距离等因素确定运输方式：冷冻的细胞冻存管(干冰)、一小瓶贴壁培养的细胞或一小瓶/管悬浮培养的细胞(常温)。为了更好地耐受长途运输和环境温度等变化，对于正常贴壁培养的细胞，也可能会以悬浮的形式培养在细胞培养瓶或离心管中进行运输。
对于干冰运输的冻存细胞，若干冰已经完全融化，请立即将细胞复苏培养，切勿再次低温冻存；若尚留有干冰，请直接复苏培养或立即将含有细胞的冻存管放入液氮中保存待用，切不可将细胞置于高温环境。
收到冻存的细胞后请尽快复苏细胞进行培养，以确认细胞活力、状态并保种。如暂时不进行复苏操作，冻存细胞可在-80℃条件下保存2个月。
每支冻存管约含1×106个细胞，体积为0.5-1ml，预期存活率60-90%，建议复苏至1个6cm培养皿中。如果复苏后存活率较低，可以消化后转移至3.5cm培养皿中，这样细胞生长会更好。
如果本产品是常温运输，并且是培养瓶中充满完全培养液的贴壁细胞，收到细胞后请在显微镜下观察细胞生长状态，如果细胞密度超过85%请尽快进行传代操作；如果悬浮的细胞较多，请将培养瓶置于培养箱中静置过夜以使悬浮的细胞再次贴壁。如果收到的是常温运输的离心管装的悬浮细胞，可以直接取出转移至培养皿或培养瓶中培养。若培养液颜色正常则保留培养液继续培养，并且在首次更换培养液时，保留一半原培养液，并加入一半新鲜培养液，这样可以尽量避免由于培养液或血清差异导致细胞生长的不适应，确保细胞良好的生长状态。
细胞培养请在生物安全柜台中进行操作，并严格遵守无菌操作。
请在培养液中加入适量青霉素-链霉素溶液以防止可能的细菌污染，如碧云天的青霉素-链霉素溶液(100X) (C0222)。
理论上永生化细胞可无限传代，但为了保证细胞的良好状态，建议最早培养的几代细胞就冻存一批，并每培养一段时间后复苏早期冻存的细胞进行培养。
接收、处理、保存、丢弃及使用细胞的时候要遵守相关法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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