碧云天生物技术-PCR纯化试剂盒/DNA纯化试剂盒(D0033)

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PCR纯化试剂盒/DNA纯化试剂盒

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产品编号: D0033

产品包装:50次

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产品编号	产品名称	产品包装	产品价格
D0033	PCR纯化试剂盒/DNA纯化试剂盒	50次	156.00元

碧云天的PCR纯化试剂盒(PCR Clean Up Kit)，也称DNA纯化试剂盒(DNA Purification Kit)是一种用于PCR产物纯化或从多种DNA反应体系中纯化DNA的试剂盒。
适用于PCR反应后去除引物、酶、矿物油、甘油、盐等杂质；也同样适用于酶切、连接、磷酸化、补平或切平、随机引物等反应后的DNA纯化。纯化所得DNA可直接用于酶切、连接、转化细菌、测序、PCR、杂交等后续操作。本试剂盒适用于纯化100bp-10kb DNA，长至30个碱基的引物均可被完全去除。
本试剂盒采用了一种新型的DNA纯化柱。在特定条件下，使DNA能在离心过柱的瞬间，结合到DNA纯化柱上，在一定条件下又能将DNA充分洗脱，从而实现DNA的快速纯化。无需酚氯仿抽提，无需酒精沉淀，12个样品只需不足15分钟即可完成。
每个DNA纯化柱可以结合的DNA量的上限约为15微克。
DNA回收效率约为90%。接近100bp或10kb的DNA片段回收效率要略低一些，大于10kb的DNA回收效率迅速下降。另外如果样品中DNA含量特别低也会导致回收效率下降。本试剂盒的纯化效果参见图1。

图1. 本试剂盒纯化效果图。本图仅作参考，不同的样品不同的纯化条件，实际的纯化效果可能和上图有一定的差别。

本试剂盒足够纯化50个平均体积不超过400微升的DNA样品。
包装清单：

产品编号	产品名称	包装
D0033-1	溶液I (DNA纯化结合液)	20ml
D0033-2	溶液II (洗涤液)	26ml (第一次使用前加入39ml无水乙醇)
D0033-3	溶液III (洗脱液)	3ml
D0033-4	DNA纯化柱及废液收集管	50套
—	说明书	1份

保存条件：
室温保存，一年有效。
注意事项：
第一次使用前在溶液II(洗涤液)中加入39ml无水乙醇，混匀，并在瓶上做好标记。
溶液I对人体有刺激性，操作时请小心，并注意适当防护以避免直接接触人体或吸入体内。
本试剂盒所有操作均在室温进行，操作时无需冰浴。所有离心也均在室温进行。
废液收集管在一次抽提中需多次使用，切勿中途丢弃。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 加入等体积的溶液I，混匀。
例如DNA样品体积为100微升，则加100微升溶液I。如果等体积混合后体积较大，可以把部分样品加入到纯化柱内，经离心处理后，再加入剩余的样品继续处理。矿物油对于本试剂盒没有干扰。
2. 把与溶液I等体积混匀的样品加入到DNA纯化柱内，室温放置1分钟。通常室温放置数秒待溶液浸润到纯化柱中即可。
3. 最高速(16,000g，约12,000-14,000rmp左右)离心1分钟，倒弃收集管内的液体。
注意：这一步尽量要达到16,000g，较低离心速度会导致回收效率下降。
4. 在DNA纯化柱内加入700微升溶液II，室温放置1分钟。
5. 最高速离心1分钟，洗去杂质。倒弃收集管内的液体。
6. 再加入500微升溶液II，最高速离心1分钟，进一步洗去杂质。倒弃收集管内的液体。
7. 最高速再离心1分钟，除去残留液体并让残留的乙醇充分挥发。
8. 将DNA纯化柱置于1.5毫升离心管上，加入50微升溶液III至管内柱面上，放置1分钟。
用1.5ml离心管作为收集管。溶液III需要直接加至管内柱面中央，使液体被纯化柱吸收。如果不慎将溶液III沾在管壁上，一定要震动管子，使液体滑落到管底，以便被纯化柱吸收。也可以用重蒸水或Milli-Q级纯水替代溶液III，但是水的pH值应不小于6.5。如需得到较高浓度的DNA，可以只加20-30微升溶液III，但产量会略有下降。放置较长时间例如3-5分钟，会对提高产量略有帮助。
9. 最高速离心1分钟，所得液体即为高纯度DNA。

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