碧云天生物技术-pGL6 (报告基因质粒)(D2102-100μg)

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pGL6 (报告基因质粒)

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产品编号: D2102-100μg

产品包装:100μg

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产品编号	产品名称	产品包装	产品价格
D2102-1μg	pGL6 (报告基因质粒)	1μg	888.00元
D2102-100μg	pGL6 (报告基因质粒)	100μg	1169.00元

      pGL6 (报告基因质粒)是碧云天自行研发的用于在哺乳动物细胞中进行萤火虫萤光素酶(firefly luciferase)报告基因检测的新一代质粒。该报告基因质粒比Promega公司的pGL3系列有了全面的改进，一方面对于luciferase的编码进行了改进，确保能更好地在哺乳动物细胞中进行表达，同时对整个质粒中所有可以被预测出的可能的转录因子结合位点全部进行了适当的突变处理，在保持原有功能不变的情况下，使各种转录因子在质粒上的非特异性结合降到最低。
      萤光素、萤光素酶、萤火虫萤光素酶和海肾萤光素酶也经常被称作荧光素、荧光素酶、萤火虫荧光素酶和海肾荧光素酶。
      pGL6主要用于在其多克隆位点插入特定的启动子、增强子等调控元件研究该调控序列的基因转录调控活性。
      pGL6质粒的主要信息如下：

Base pairs	5588
Multiple cloning region	1-59
luc2 reporter gene	89-1741
SV40 late poly(A) signal	1776-1997
SV40 early enhancer/promoter	2045-2463
Synthetic neomycin phosphotransferase(Neor)coding region	2488-3282
Synthetic poly(A) signal	3307-3355
Reporter Vector primer 4 (RVprimer4) binding region	3422-3441
ColE1-derived plasmid replication origin	3679
Synthetic Beta-lactamase (Ampr) coding region	4470-5330
Synthetic poly(A) signal/transcriptional pause site	5435-5588
Reporter Vector primer 3 (RVprimer3) binding region	5537-5556

pGL6质粒的图谱如下：

pGL6的多克隆位点的详细图谱见说明书：https://www.beyotime.com/Manual/D2102 pGL6 (报告基因质粒).pdf

pGL6中没有的酶切位点(Restriction enzymes that do not cut pGL6)包括：

Aat II	Afl II	Asc I	Ase I	Bsa I	BsaA I	BsiW I
BspM II	BssH II	Eco72 I	EcoR I	EcoR V	Nde I	Nru I
PflM I	Pme I	Pml I	Psp1406 I	PspA I	Rsr II
Sma I	SnaB I	Spl I	Srf I	Tth111 I

pGL6中的单酶切位点(Restriction enzymes that cut pGL6 once)包括：

Sfi I	GGCCN,NNN`NGGCC	9	Stu I	AGG\|CCT	2442
Bgl I	GCCN,NNN`NGGC	9	EcoN I	CCTNN`N,NNAGG	2963
Acc65 I	G`GTAC,C	15	BsiC I	TT`CG,AA	3358
Asp718	G`GTAC,C	15	BstB I	TT`CG,AA	3358
Kpn I	G,GTAC`C	19	Sal I	G`TCGA,C	3372
Nhe I	G`CTAG,C	21	ApaL I	G`TGCA,C	3936
PaeR7 I	C`TCGA,G	27	HgiE II	ACCNNNNNNGGT -1/13	4201
Xho I	C`TCGA,G	27	Not I	GC`GGCC,GC	4442
Sac I	G,AGCT`C	37	BstX I	CCAN,NNNN`NTGG	4466
Mlu I	A`CGCG,T	39	BstE II	G`GTNAC,C	4469
Bgl II	A`GATC,T	45	Ahd I	GACNN,N`NNGTC	4544
Hind III	A`AGCT,T	55	Bsu36 I	CC`TNA,GG	4900
BsrG I	T`GTAC,A	580	Pvu I	CG,AT`CG	4914
Dra III	CAC,NNN`GTG	1236	Sac II	CC,GC`GG	4938
Gsu I	CTGGAG 21/19	1469	Bst1107 I	GTA\|TAC	5054
Bpm I	CTGGAG 22/20	1470	Xca I	GTA\|TAC	5054
Apo I	R`AATT,Y	1852	Spe I	A`CTAG,T	5373
Mun I	C`AATT,G	1916	BsmA I	GTCTC`/9	5385
BamH I	G`GATC,C	2009	BsmB I	CGTCTC 7/11	5386

      pGL6质粒中推荐使用的测序引物序列如下：
      RVprimer3(5537-5556)：
      CTA GCA AAA TAG GCT GTC CC
      pGL6的全序列信息：d2102-pGL6-seq.txt
包装清单：

产品编号	产品名称	包装
D2102	pGL6 (报告基因质粒)	1μg
—	说明书	1份

保存条件：
      -20℃保存。
注意事项：
      本质粒未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。
      本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
      为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 首次使用时请先取少量本质粒转化大肠杆菌，进行质粒小量、中量或大量抽提后再用于后续用途。抽提获得的质粒可以通过酶切电泳进行鉴定，或通过测序进行鉴定。
2. 用于插入调控序列：在多克隆位点选取适当的酶切位点，经酶切处理后连入适当的基因转录调控序列。pGL6也可以用作报告基因检测时的阴性对照。
3. pGL6质粒以及以此质粒为模板构建的质粒可以用常规的细胞转染方法转染细胞。检测时可以采用碧云天的萤火虫萤光素酶报告基因检测试剂盒(RG005/RG006)或双萤光素酶报告基因检测试剂盒(RG027/RG028)。

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