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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| D6018 | 10X CutEZ™ Buffer | 5ml | 68.00元 |
碧云天生产的10X CutEZ™ Buffer是一种能让超过200种限制性内切酶的活性达到100%的缓冲液。使用CutEZ™ Buffer，可以免去内切酶酶切时选择buffer的麻烦，特别是在双酶切时会变得更加简单易行。此外，许多DNA修饰酶在CutEZ™ Buffer中也保留了100%酶活性，这样在酶切后进行DNA修饰酶处理就无需进行DNA纯化了。
各种内切酶和修饰酶在1X CutEZ™ buffer中的活性，请参考附录1和附录2。
不同来源的限制性内切酶对于CutEZ™ Buffer的兼容性可能略有不同。
1X CutEZ™ Buffer的组分为50 mM Potassium acetate, 20 mM Tris-acetate, 10 mM Magnesium acetate, 100 μg/ml BSA, pH 7.9 @ 25℃，与NEB公司的CutSmart™ Buffer完全一致。
一个包装的本产品，如果用于20μl的酶切反应体系，共可以用于2500个酶切反应。
包装清单：
| 产品编号 | 产品名称 | 包装 |
| D6018 | 10X CutEZ™ Buffer | 1ml×5 |
| — | 说明书 | 1份 |
保存条件：
-20℃保存。
注意事项：
使用前，反应缓冲液应彻底融化并混匀。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1．根据酶切反应体系加入总体积1/10的10X CutEZ™ Buffer，使其终浓度为1X，待所有组分都加入完毕，混匀后即可置于适当的温度中进行酶切反应。
附录1：
常用限制性内切酶在CutEZ™ Buffer中的活性和效能表如下：
| Enzyme | Sequence | % Activity in CutEZ™ | Heat Inac. | Incu. Temp. | Dam | Dcm | CpG | Unit Substrate |
| AatII | GACGT/C | 100 | 80℃ | 37℃ | ● | ● | ■ | λ DNA |
| Acc65I | G/GTACC | 25 | 65℃ | 37℃ | ● | □ scol | □ scol | pBC4 DNA |
| AccI | GT/MKAC | 100 | 80℃ | 37℃ | ● | ● | □ ol | λ DNA |
| AciI | CCGC(-3/-1) | 100 | 65℃ | 37℃ | ● | ● | ■ | λ DNA |
| AclI | AA/CGTT | 100 | No | 37℃ | ● | ● | ■ | λ DNA |
| AcuI | CTGAAG(16/14) | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| AfeI | AGC/GCT | 100 | 65℃ | 37℃ | ● | ● | ■ | pXba DNA |
| AflII | C/TTAAG | 100 | 65℃ | 37℃ | ● | ● | ● | ΦX174 RF I DNA |
| AflIII | A/CRYGT | 50 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| AgeI | A/CCGGT | 75 | 65℃ | 37℃ | ● | ● | ■ | λ DNA |
| AhdI | GACNNN/NNGTC | 100 | 65℃ | 37℃ | ● | ● | ◊ scol | λ DNA |
| AleI | CACNN/NNGTG | 100 | 80℃ | 37℃ | ● | ● | ◊ scol | λ DNA |
| AluI | AG/CT | 100 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| AlwI | GGATC(4/5) | 100 | No | 37℃ | ■ | ● | ● | λ DNA (dam-) |
| AlwNI | CAGNNN/CTG | 100 | 80℃ | 37℃ | ● | □ ol | ● | λ DNA |
| ApaI | GGGCC/C | 100 | 65℃ | 25℃ | ● | □ ol | □ ol | pXba DNA |
| ApaLI | G/TGCAC | 100 | No | 37℃ | ● | ● | □ ol | λ DNA/HindIII |
| ApeKI | G/CWGC | 10 | No | 75℃ | ● | ● | λ DNA | |
| ApoI | R/AATTY | 75 | 80℃ | 50℃ | ● | ● | ● | λ DNA |
| AscI | GG/CGCGCC | 100 | 80℃ | 37℃ | ● | ● | ■ | λ DNA |
| AseI | AT/TAAT | 10 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| AsiSI | GCGAT/CGC | 100 | 80℃ | 37℃ | ● | ● | ■ | pXba/XhoI |
| AvaI | C/YCGRG | 100 | 80℃ | 37℃ | ● | ● | ■ | λ DNA |
| AvaII | G/GWCC | 100 | 80℃ | 37℃ | ● | □ ol | □ ol | λ DNA |
| AvrII | C/CTAGG | 100 | No | 37℃ | ● | ● | ● | λ DNA/HindIII |
| BaeI | (10/15)ACNNNNGTAYC(12/7) | 100 | 65℃ | 25℃ | ● | ● | □ scol | pXba DNA |
| BamHI | G/GATCC | 100* | No | 37℃ | ● | ● | ● | λ DNA |
| BanI | G/GYRCC | 100 | 65℃ | 37℃ | ● | □ scol | □ scol | λ DNA |
| BanII | GRGCY/C | 100 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| BbsI | GAAGAC(2/6) | 75 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| BbvCI | CCTCAGC(-5/-2) | 100 | No | 37℃ | ● | ● | ◊ ol | λ DNA |
| BbvI | GCAGC(8/12) | 100 | 65℃ | 37℃ | ● | ● | ● | pBR322 (dcm+) DNA |
| BccI | CCATC(4/5) | 100 | 65℃ | 37℃ | ● | ● | ● | pXba DNA |
| BceAI | ACGGC(12/14) | 100* | 65℃ | 37℃ | ● | ● | ■ | pBR322 (dcm+) DNA |
| BcgI | (10/12)CGANNNNNNTGC(12/10) | 50* | 65℃ | 37℃ | ◊ ol | ● | □ scol | λ DNA |
| BciVI | GTATCC(6/5) | 100 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| BclI | T/GATCA | 75 | No | 50℃ | ■ | ● | ● | λ DNA (dam-) |
| BfaI | C/TAG | 100 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| BfuAI | ACCTGC(4/8) | 10 | 65℃ | 50℃ | ● | ● | ◊ ol | λ DNA |
| BglI | GCCNNNN/NGGC | 10 | 65℃ | 37℃ | ● | ● | □ scol | λ DNA |
| BglII | A/GATCT | 10 | No | 37℃ | ● | ● | ● | λ DNA |
| BlpI | GC/TNAGC | 100 | No | 37℃ | ● | ● | ● | λ DNA |
| BmgBI | CACGTC(-3/-3) | 10 | 65℃ | 37℃ | ● | ● | ■ | λ DNA |
| BmrI | ACTGGG(5/4) | 100* | 65℃ | 37℃ | ● | ● | ● | λ DNA/HindIII |
| BmtI | GCTAG/C | 100 | 65℃ | 37℃ | ● | ● | ● | pXba DNA |
| BpmI | CTGGAG(16/14) | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| Bpu10I | CCTNAGC(-5/-2) | 25 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| BpuEI | CTTGAG(16/14) | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| BsaAI | YAC/GTR | 100 | No | 37℃ | ● | ● | ■ | λ DNA |
| BsaBI | GATNN/NNATC | 100 | 80℃ | 60℃ | □ ol | ● | □ scol | λ DNA (dam-) |
| BsaHI | GR/CGYC | 100 | 80℃ | 37℃ | ● | □ scol | ■ | λ DNA |
| BsaI | GGTCTC(1/5) | 100 | 65℃ | 37℃ | ● | ◊ scol | □ scol | pXba DNA |
| BsaJI | C/CNNGG | 100 | 80℃ | 60℃ | ● | ● | ● | λ DNA |
| BsaWI | W/CCGGW | 100 | 80℃ | 60℃ | ● | ● | ● | λ DNA |
| BsaXI | (9/12)ACNNNNNCTCC(10/7) | 100 | No | 37℃ | ● | ● | ● | λ DNA |
| BseRI | GAGGAG(10/8) | 100 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| BseYI | CCCAGC(-5/-1) | 50 | 80℃ | 37℃ | ● | ● | □ ol | λ DNA |
| BsgI | GTGCAG(16/14) | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| BsiEI | CGRY/CG | 100 | No | 60℃ | ● | ● | ■ | λ DNA |
| BsiHKAI | GWGCW/C | 100 | No | 65℃ | ● | ● | ● | λ DNA |
| BsiWI | C/GTACG | 25 | 65℃ | 55℃ | ● | ● | ■ | ΦX174 DNA |
| BslI | CCNNNNN/NNGG | 100 | No | 55℃ | ● | □ scol | □ scol | λ DNA |
| BsmAI | GTCTC(1/5) | 100 | No | 55℃ | ● | ● | □ scol | λ DNA |
| BsmBI | CGTCTC(1/5) | 25 | 80℃ | 55℃ | ● | ● | ■ | λ DNA |
| BsmFI | GGGAC(10/14) | 100 | 80℃ | 65℃ | ● | □ ol | □ ol | pBR322 (dcm+) DNA |
| BsmI | GAATGC(1/-1) | 100 | 80℃ | 65℃ | ● | ● | ● | λ DNA |
| BsoBI | C/YCGRG | 100 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| Bsp1286I | GDGCH/C | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| BspCNI | CTCAG(9/7) | 100 | 80℃ | 25℃ | ● | ● | ● | λ DNA |
| BspDI | AT/CGAT | 100 | 80℃ | 37℃ | □ ol | ● | ■ | λ DNA |
| BspEI | T/CCGGA | 10 | 80℃ | 37℃ | □ ol | ● | ♦ | λ DNA (dam-) |
| BspHI | T/CATGA | 100 | 80℃ | 37℃ | ◊ ol | ● | ● | λ DNA |
| BspMI | ACCTGC(4/8) | 10 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| BspQI | GCTCTTC(1/4) | 100 | 80℃ | 50℃ | ● | ● | ● | λ DNA |
| BsrBI | CCGCTC(-3/-3) | 100 | 80℃ | 37℃ | ● | ● | □ scol | λ DNA |
| BsrDI | GCAATG(2/0) | 25 | 80℃ | 65℃ | ● | ● | ● | λ DNA |
| BsrFI | R/CCGGY | 100 | No | 37℃ | ● | ● | ■ | pBR322 (dcm+) DNA |
| BsrGI | T/GTACA | 25 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| BsrI | ACTGG(1/-1) | 10 | 80℃ | 65℃ | ● | ● | ● | ΦX174 RF I DNA |
| BssHII | G/CGCGC | 100 | 65℃ | 50℃ | ● | ● | ■ | λ DNA |
| BssKI | /CCNGG | 100 | 80℃ | 60℃ | ● | □ ol | □ ol | λ DNA |
| BssSI | CACGAG(-5/-1) | 50 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| BstAPI | GCANNNN/NTGC | 100 | 80℃ | 60℃ | ● | ● | □ scol | λ DNA |
| BstBI | TT/CGAA | 100 | No | 65℃ | ● | ● | ■ | λ DNA |
| BstEII | G/GTNACC | 75* | No | 60℃ | ● | ● | ● | λ DNA |
| BstNI | CC/WGG | 75 | No | 60℃ | ● | ● | ● | λ DNA |
| BstUI | CG/CG | 100 | No | 60℃ | ● | ● | ■ | λ DNA |
| BstXI | CCANNNNN/NTGG | 25 | 80℃ | 37℃ | ● | □ scol | ● | λ DNA |
| BstYI | R/GATCY | 100 | No | 60℃ | ● | ● | ● | λ DNA |
| BstZ17I | GTA/TAC | 100 | No | 37℃ | ● | ● | □ scol | λ DNA |
| Bsu36I | CC/TNAGG | 100 | 80℃ | 37℃ | ● | ● | ● | λ DNA/HindIII |
| BtgI | C/CRYGG | 100 | 80℃ | 37℃ | ● | ● | ● | pBR322 (dcm+) DNA |
| BtgZI | GCGATG(10/14) | 100 | 80℃ | 60℃ | ● | ● | ♦ | λ DNA |
| BtsCI | GGATG(2/0) | 100 | 80℃ | 50℃ | ● | ● | ● | λ DNA |
| BtsI | GCAGTG(2/0) | 100 | 80℃ | 55℃ | ● | ● | ● | λ DNA |
| Cac8I | GCN/NGC | 100 | 65℃ | 37℃ | ● | ● | □ scol | λ DNA |
| ClaI | AT/CGAT | 100 | 65℃ | 37℃ | □ ol | ● | ■ | λ DNA (dam-) |
| CspCI | (11/13)CAANNNNNGTGG(12/10) | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| CviAII | C/ATG | 100 | 65℃ | 25℃ | ● | ● | ● | λ DNA |
| CviKI-1 | RG/CY | 100 | No | 37℃ | ● | ● | ● | pBR322 (dcm+) DNA |
| CviQI | G/TAC | 75* | No | 25℃ | ● | ● | ● | λ DNA |
| DdeI | C/TNAG | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| DpnI | GA/TC | 100 | 80℃ | 37℃ | ● | ● | □ ol | pBR322 DNA |
| DpnII | /GATC | 25 | 65℃ | 37℃ | ■ | ● | ● | λ DNA (dam-) |
| DraI | TTT/AAA | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| DraIII | CACNNN/GTG | 25 | 65℃ | 37℃ | ● | ● | ◊ ol | λ DNA |
| DrdI | GACNNNN/NNGTC | 100 | 65℃ | 37℃ | ● | ● | □ scol | pUC19 DNA |
| EaeI | Y/GGCCR | 100 | 65℃ | 37℃ | ● | □ ol | □ ol | λ DNA |
| EagI | C/GGCCG | 10 | 65℃ | 37℃ | ● | ● | ■ | pXba DNA |
| EarI | CTCTTC(1/4) | 100 | 65℃ | 37℃ | ● | ● | ◊ ol | λ DNA |
| EciI | GGCGGA(11/9) | 100 | 65℃ | 37℃ | ● | ● | □ scol | λ DNA |
| EcoNI | CCTNN/NNNAGG | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| EcoO109I | RG/GNCCY | 100 | 65℃ | 37℃ | ● | □ ol | ● | λ DNA/HindIII |
| EcoP15I | CAGCAG(25/27) | 100 | 65℃ | 37℃ | ● | ● | ● | pUC19 DNA |
| EcoRI | G/AATTC | 50* | 65℃ | 37℃ | ● | ● | □ scol | λ DNA |
| EcoRV | GAT/ATC | 10 | 80℃ | 37℃ | ● | ● | ◊ scol | λ DNA |
| FatI | /CATG | 50 | 80℃ | 55℃ | ● | ● | ● | pUC19 DNA |
| FauI | CCCGC(4/6) | 100 | 65℃ | 55℃ | ● | ● | ■ | λ DNA |
| Fnu4HI | GC/NGC | 100 | No | 37℃ | ● | ● | □ ol | λ DNA |
| FokI | GGATG(9/13) | 100 | 65℃ | 37℃ | ● | ◊ ol | ◊ ol | λ DNA |
| FseI | GGCCGG/CC | 100 | 65℃ | 37℃ | ● | ◊ scol | ■ | Adenovirus-2 DNA |
| FspI | TGC/GCA | 100 | No | 37℃ | ● | ● | ■ | λ DNA |
| HaeII | RGCGC/Y | 100 | 80℃ | 37℃ | ● | ● | ■ | λ DNA |
| HaeIII | GG/CC | 100 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| HgaI | GACGC(5/10) | 100 | 65℃ | 37℃ | ● | ● | ■ | ΦX174 DNA |
| HhaI | GCG/C | 100 | 65℃ | 37℃ | ● | ● | ■ | λ DNA |
| HincII | GTY/RAC | 100 | 65℃ | 37℃ | ● | ● | □ scol | λ DNA |
| HindIII | A/AGCTT | 50 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| HinfI | G/ANTC | 100 | 80℃ | 37℃ | ● | ● | □ scol | λ DNA |
| HinP1I | G/CGC | 100 | 65℃ | 37℃ | ● | ● | ■ | λ DNA |
| HpaI | GTT/AAC | 100 | No | 37℃ | ● | ● | □ scol | λ DNA |
| HpaII | C/CGG | 100 | 80℃ | 37℃ | ● | ● | ■ | λ DNA |
| HphI | GGTGA(8/7) | 100 | 65℃ | 37℃ | ■ | ■ | ● | λ DNA |
| Hpy188I | TCN/GA | 100 | 65℃ | 37℃ | □ scol | ● | ● | pBR322 (dcm+) |
| Hpy99I | CGWCG/ | 100 | 65℃ | 37℃ | ● | ● | ■ | λ DNA |
| HpyAV | CCTTC(6/5) | 100 | 65℃ | 37℃ | ● | ● | ◊ ol | λ DNA |
| HpyCH4III | ACN/GT | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| HpyCH4I | A/CGT | 100 | 65℃ | 37℃ | ● | ● | ■ | pUC19 DNA |
| HpyCH4V | TG/CA | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| KasI | G/GCGCC | 100 | 65℃ | 37℃ | ● | ● | ■ | pBR322 DNA |
| KpnI | GGTAC/C | 50* | No | 37℃ | ● | ● | ● | pXba DNA |
| MboI | /GATC | 100 | 65℃ | 37℃ | ■ | ● | ◊ ol | λ DNA (dam-) |
| MboII | GAAGA(8/7) | 100 | 65℃ | 37℃ | □ ol | ● | ● | λ DNA (dam-) |
| MfeI | C/AATTG | 100 | No | 37℃ | ● | ● | ● | λ DNA |
| MluI | A/CGCGT | 25 | 80℃ | 37℃ | ● | ● | ■ | λ DNA |
| MlyI | GAGTC(5/5) | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| MmeI | TCCRAC(20/18) | 100 | 65℃ | 37℃ | ● | ● | □ ol | ΦX174 DNA |
| MnlI | CCTC(7/6) | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| MscI | TGG/CCA | 100 | 80℃ | 37℃ | ● | □ ol | ● | λ DNA |
| MseI | T/TAA | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| MslI | CAYNN/NNRTG | 100 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| MspA1I | CMG/CKG | 100 | 65℃ | 37℃ | ● | ● | □ ol | λ DNA |
| MspI | C/CGG | 100 | No | 37℃ | ● | ● | ● | λ DNA |
| MwoI | GCNNNNN/NNGC | 100 | No | 60℃ | ● | ● | □ scol | λ DNA |
| NaeI | GCC/GGC | 100 | No | 37℃ | ● | ● | ■ | pXba DNA |
| NarI | GG/CGCC | 100 | 65℃ | 37℃ | ● | ● | ■ | pXba DNA |
| NciI | CC/SGG | 100 | No | 37℃ | ● | ● | ◊ ol | λ DNA |
| NcoI | C/CATGG | 100 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| NdeI | CA/TATG | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| NgoMIV | G/CCGGC | 100 | No | 37℃ | ● | ● | ■ | Adenovirus-2 DNA |
| NheI | G/CTAGC | 100 | 65℃ | 37℃ | ● | ● | □ scol | λ DNA/HindIII |
| NlaIII | CATG/ | 100 | 65℃ | 37℃ | ● | ● | ● | ΦX174 DNA |
| NlaIV | GGN/NCC | 100 | 65℃ | 37℃ | ● | □ ol | □ ol | pBR322 DNA |
| NmeAIII | GCCGAG(21/19) | 100 | 65℃ | 37℃ | ● | ● | ● | ΦX174 RF I DNA |
| NotI | GC/GGCCGC | 25 | 65℃ | 37℃ | ● | ● | ■ | pBC4 DNA |
| NruI | TCG/CGA | 10 | No | 37℃ | □ ol | ● | ■ | λ DNA |
| NsiI | ATGCA/T | 25 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| NspI | RCATG/Y | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| PacI | TTAAT/TAA | 100 | 65℃ | 37℃ | ● | ● | ● | pNEB193 DNA |
| PaeR7I | C/TCGAG | 100 | No | 37℃ | ● | ● | ■ | λ DNA/HindIII |
| PciI | A/CATGT | 50* | 80℃ | 37℃ | ● | ● | ● | pXba DNA |
| PflFI | GACN/NNGTC | 100 | 65℃ | 37℃ | ● | ● | ● | pBC4 DNA |
| PflMI | CCANNNN/NTGG | 50 | 65℃ | 37℃ | ● | □ ol | ● | λ DNA |
| PhoI | GG/CC | 100 | No | 37℃ | ● | ◊ scol | ◊ scol | λ DNA |
| PleI | GAGTC(4/5) | 100 | 65℃ | 37℃ | ● | ● | □ scol | λ DNA |
| PmeI | GTTT/AAAC | 100 | 65℃ | 37℃ | ● | ● | □ scol | λ DNA |
| PmlI | CAC/GTG | 100 | 65℃ | 37℃ | ● | ● | ■ | λ DNA/HindIII |
| PpuMI | RG/GWCCY | 100 | No | 37℃ | ● | □ ol | ● | λ DNA/HindIII |
| PshAI | GACNN/NNGTC | 100 | 65℃ | 37℃ | ● | ● | □ scol | λ DNA |
| PsiI | TTA/TAA | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| PspGI | /CCWGG | 100 | No | 75℃ | ● | ■ | ● | T7 DNA |
| PspOMI | G/GGCCC | 100 | 65℃ | 37℃ | ● | ◊ scol | □ ol | pXba DNA |
| PspXI | VC/TCGAGB | 100 | No | 37℃ | ● | ● | ♦ | λ DNA/HindIII |
| PstI | CTGCA/G | 50* | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| PvuI | CGAT/CG | 10 | No | 37℃ | ● | ● | ■ | pXba DNA |
| PvuII | CAG/CTG | 100* | No | 37℃ | ● | ● | ● | λ DNA |
| RsaI | GT/AC | 100 | No | 37℃ | ● | ● | □ scol | λ DNA |
| RsrII | CG/GWCCG | 100 | 65℃ | 37℃ | ● | ● | ■ | λ DNA |
| SacI | GAGCT/C | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA/HindIII |
| SacII | CCGC/GG | 100 | 65℃ | 37℃ | ● | ● | ■ | pXba DNA |
| SalI | G/TCGAC | 10 | 65℃ | 37℃ | ● | ● | ■ | λ DNA/HindIII |
| SapI | GCTCTTC(1/4) | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| Sau3AI | /GATC | 100 | 65℃ | 37℃ | ● | ● | □ ol | λ DNA |
| Sau96I | G/GNCC | 100 | 65℃ | 37℃ | ● | □ ol | □ ol | λ DNA |
| SbfI | CCTGCA/GG | 100 | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| ScaI | AGT/ACT | N/R | 80℃ | 37℃ | ● | ● | ● | λ DNA |
| ScrFI | CC/NGG | 100 | 65℃ | 37℃ | ● | □ ol | □ ol | λ DNA |
| SexAI | A/CCWGGT | 100 | 65℃ | 37℃ | ● | ■ | ● | pBC4 DNA (dcm-) |
| SfaNI | GCATC(5/9) | 25 | 65℃ | 37℃ | ● | ● | ◊ scol | ΦX174 RF I DNA |
| SfcI | C/TRYAG | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| SfiI | GGCCNNNN/NGGCC | 100 | No | 50℃ | ● | ◊ ol | □ scol | Adenovirus-2 DNA |
| SfoI | GGC/GCC | 100 | No | 37℃ | ● | □ scol | ■ | λ DNA/HindIII |
| SgrAI | CR/CCGGYG | 100 | 65℃ | 37℃ | ● | ● | ■ | λ DNA |
| SmaI | CCC/GGG | 100 | 65℃ | 25℃ | ● | ● | ■ | λ DNA |
| SmlI | C/TYRAG | 100 | No | 55℃ | ● | ● | ● | λ DNA |
| SnaBI | TAC/GTA | 100 | 80℃ | 37℃ | ● | ● | ■ | T7 DNA |
| SpeI | A/CTAGT | 100 | 80℃ | 37℃ | ● | ● | ● | Adenovirus-2 DNA |
| SphI | GCATG/C | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| SspI | AAT/ATT | 50 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| StuI | AGG/CCT | 100 | No | 37℃ | ● | □ ol | ● | λ DNA |
| StyD4I | /CCNGG | 100 | 65℃ | 37℃ | ● | □ ol | ◊ ol | λ DNA |
| StyI | C/CWWGG | 10 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| SwaI | ATTT/AAAT | 10 | 65℃ | 25℃ | ● | ● | ● | M13mp19 RF I DNA |
| TaqαI | T/CGA | 100 | 80℃ | 65℃ | □ ol | ● | ● | λ DNA |
| TfiI | G/AWTC | 100 | No | 65℃ | ● | ● | □ scol | λ DNA |
| TliI | C/TCGAG | 50 | No | 75℃ | ● | ● | ♦ | λ DNA/HindIII |
| TseI | G/CWGC | 100 | No | 65℃ | ● | ● | □ scol | λ DNA |
| Tsp45I | /GTSAC | 100 | No | 65℃ | ● | ● | ● | λ DNA |
| Tsp509I | /AATT | N/R | No | 65℃ | ● | ● | ● | λ DNA |
| TspMI | C/CCGGG | 100 | No | 75℃ | ● | ● | ■ | pUCAdeno |
| TspRI | NNCASTGNN/ | 100 | No | 65℃ | ● | ● | ● | λ DNA |
| Tth111I | GACN/NNGTC | 100 | No | 65℃ | ● | ● | ● | pBC4 DNA |
| XbaI | T/CTAGA | 100 | 65℃ | 37℃ | □ ol | ● | ● | λ DNA(dam-)/Hind III |
| XcmI | CCANNNNN/NNNNTGG | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| XhoI | C/TCGAG | 100 | 65℃ | 37℃ | ● | ● | ♦ | λ DNA/HindIII |
| XmaI | C/CCGGG | 100 | 65℃ | 37℃ | ● | ● | ♦ | Adenovirus-2 DNA |
| XmnI | GAANN/NNTTC | 100 | 65℃ | 37℃ | ● | ● | ● | λ DNA |
| ZraI | GAC/GTC | 100 | 80℃ | 37℃ | ● | ● | ■ | λ DNA |
注：*，在此buffer中可能产生星号活性。λ DNA/HindIII:λ DNA digested with HindIII.
● 不敏感
■ 完全阻断
□ ol 重叠性阻断
□ scol 某些重叠组合后被阻断
♦ 部分阻断
◊ ol 重叠性部分阻断
◊ scol 某些重叠组合后被部分阻断
附录2：
DNA修饰酶在CutEZ™ Buffer中的活性表格如下:
| Enzyme | % Activity in CutEZ™ | Required Supplements |
| Alkaline Phosphatase(CIP) | 100 | - |
| Antarctic Phosphatase | 100 | Zn2+ |
| Bst DNA Polymerase | 100 | - |
| CpG Methyltransferase(M.SssI) | 100 | - |
| DNA Polymerase Ⅰ | 100 | - |
| DNA PolymeraseⅠ, Large(klenow)Fragment | 100 | - |
| DNA Polymerase Klenow Exo- | 100 | - |
| DNaseⅠ(RNase-free) | 100 | Ca2+ |
| E.coli DNA Ligase | 100 | NAD |
| Endonuclease Ⅲ(Nth), recombinant | 100 | - |
| Endonuclease VIII | 100 | - |
| Exonuclease Ⅲ | 100 | - |
| GpC Methyltransferase(M.CviPI) | 0-50 | DTT |
| McrBC | 100 | - |
| Micrococcal Nuclease | 100 | - |
| Nuclease BAL-31 | 100 | - |
| phi29 DNA Polymerase | 100 | - |
| RecJf | 100 | - |
| T3 DNA Ligase | 100 | ATP+PEG |
| T4 DNA Ligase | 100 | ATP |
| T4 DNA Polymerase | 100 | - |
| T4 Phage β-glucosyltransferase(T4-BGT) | 100 | - |
| T4 Polynucleotide Kinase | 100 | ATP |
| T4 PNK(3' phosphatase minus) | 100 | ATP |
| T7 DNA Ligase | 100 | ATP+PEG |
| T7 DNA Polymerase(unmodified) | 100 | - |
| T7 Exonuclease | 100 | - |
| USER Enzyme, recombinant | 100 | - |
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