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多肽与蛋白> 蛋白检测> 蛋白电泳
SDS-PAGE蛋白上样缓冲液(5X)
产品编号: P0015L
产品包装:15ml
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价格:¥155.00元
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产品编号 产品名称 产品包装 产品价格
P0015 SDS-PAGE蛋白上样缓冲液(5X) 2ml 35.60元
P0015L SDS-PAGE蛋白上样缓冲液(5X) 15ml 155.00元

SDS-PAGE蛋白上样缓冲液(SDS-PAGE Sample Loading Buffer,5X),是一种经过改良的以溴酚蓝为染料,5倍浓缩的蛋白上样缓冲液。
可以用于常规的SDS-PAGE蛋白样品的上样。
包装清单:

产品编号 产品名称 包装
P0015L SDS-PAGE蛋白上样缓冲液(5X) 15ml
说明书 1份

保存条件:
-20℃保存,至少一年有效。
注意事项:
SDS-PAGE蛋白上样缓冲液(5X)中含少量DTT,有轻微刺激性气味,但不含剧毒的巯基乙醇。
SDS-PAGE蛋白上样缓冲液(5X)必须完全溶解后再使用。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。


使用说明:
1. 在室温或不超过37℃的水浴中溶解SDS-PAGE上样缓冲液(5X)。水浴溶解后立即室温存放,尽量避免长时间置于水浴中。
2. 按照每4微升蛋白样品加入1微升蛋白上样缓冲液(5X)的比例,混合蛋白样品和蛋白上样缓冲液(5X)。
3. 100℃或沸水浴加热3-5分钟,以充分变性蛋白。
注意:如果起始时细胞或组织的用量较大,基因组DNA含量较高,煮沸3-5分钟后有可能仍然比较粘稠或者有粘稠状的半透明物体。此时需要再煮沸5-10分钟或者加入适量稀释成1X的蛋白上样缓冲液后再煮沸3-5分钟。充分煮沸后一方面可以使结合在基因组DNA上的蛋白充分释放,同时会导致基因组DNA的部分断裂从而使粘稠感消失,这样就不会影响后续的上样操作了。
4. 冷却到室温后,直接上样到SDS-PAGE胶加样孔内即可。
5. 通常电泳至蓝色染料到达胶的底端处附近即可停止电泳。
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