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多肽与蛋白> 蛋白检测> Western检测
产品编号: P0023A-100ml
产品包装:100ml
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价格:¥118.00元
产品简介
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产品编号 产品名称 产品包装 产品价格
P0023A-100ml Western一抗稀释液 100ml 118.00元
P0023A-500ml Western一抗稀释液 500ml 486.00元

碧云天生产的Western一抗稀释液(Primary Antibody Dilution Buffer)可以用于Western时一抗(primary antibody)的稀释和配制。使用本Western一抗稀释液稀释和配制的一抗可以在4℃保存和使用不少于1个月,可以反复多次用于Western,节约您宝贵的抗体。
本产品的主要有效成分为经过反复优化的适量BSA、适当的去垢剂和一抗稳定试剂等,一方面能有效减少一抗的非特异性结合,另一方面能有效提升稀释后一抗的稳定保存时间。
按照每个一抗稀释10毫升计算,一个100ml包装的Western一抗稀释液可以稀释10个或10次一抗,一个500ml包装的Western一抗稀释液可以稀释50个或50次一抗。
包装清单:

产品编号 产品名称 包装
P0023A-100ml Western一抗稀释液 100ml
P0023A-500ml Western一抗稀释液 500ml
说明书 1份

保存条件:
4℃保存,一年有效。长期不使用可以-20℃保存。
注意事项:
为了能使抗体可以反复多次使用,建议尽量在4℃进行一抗和蛋白膜的结合反应,以减缓一抗的衰减速度。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 参考所用的一抗的说明,以及样品中目的蛋白的含量,按照适当比例例如1:1000、1:500等比例稀释一抗。一抗稀释后即可直接用于Western。一次Western结束后,可以回收稀释的一抗,4℃保存,以用于下次的Western。
2. 详细的Western操作可以参考我们的相关网页:http://www.beyotime.com/support/western.htm

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129. Liu F,Shi J,Zhang Y,Lian A,Han X,Zuo K,Liu M,Zheng T,Zou F,Liu X,Jin M,Mu Y,Li G,Su G,Liu J
Oxid Med Cell Longev. 2019 Dec 4;2019:4286213. (IF 5.076)
130. Jing Wu,Jian-Jun Yang,Yan Cao,Huihui Li,Hongting Zhao,Shuofei Yang,Kuanyu Li
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131. Ma GW,Chu YK,Yang H,Yan XH,Rong EG,Li H,Wang N
Biochem Genet. 2020 Apr;58(2):335-347. (IF 2.027)
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