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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P5844-500μg | Recombinant Rhesus Macaque IL-3 | 500μg | 12990.00元 |
| P5844-10μg | Recombinant Rhesus Macaque IL-3 | 10μg | 1671.00元 |
| P5844-100μg | Recombinant Rhesus Macaque IL-3 | 100μg | 7363.00元 |
| P5844-1mg | Recombinant Rhesus Macaque IL-3 | 1mg | - |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Rhesus Macaque | 706946 | P25140 | E. coli | 124aa | 14kDa | － |
| About this protein | |
| Name | Recombinant Rhesus Macaque IL-3 (Recombinant Rhesus Macaque Interleukin-3; rRhIL-3)；重组恒河猴白细胞介素3 |
| Synonyms | Interleukin-3; IL-3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor; IL3 |
| Purity | >98% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Assay #1: Fully biologically active when compared to standard. The ED50 as determined by the dosedependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0ng/ml, corresponding to a specific activity of >2×105IU/mg. Assay #2: The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg. |
| Physical Appearance | Sterile Filtered lyophilized (freeze-dried) liquid-like powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, 5% trehalose. |
| Endotoxin | Less than 1EU/μg of rRhIL-3 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The rhesus macaque IL-3 reported to be a monomer, as it is known, contains 124 amino acids residues which is a single non-glycosylated polypeptide. Specifically, recombinant rhesus macaque, human and murine IL-3 share low homology. |
| Amino Acid Sequence | APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P5844-10μg | Recombinant Rhesus Macaque IL-3 | 10μg |
| P5844-100μg | Recombinant Rhesus Macaque IL-3 | 100μg |
| P5844-500μg | Recombinant Rhesus Macaque IL-3 | 500μg |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂，此时冻干后本产品看起来仍然像是液体，属于正常现象。在冻干工艺上，海藻糖作为常用的冻干保护剂，可显著阻止蛋白质二级结构的改变，防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
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