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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P5991-500μg | Recombinant Murine IL-36γ, 152a.a. | 500μg | 22520.00元 |
| P5991-10μg | Recombinant Murine IL-36γ, 152a.a. | 10μg | 1671.00元 |
| P5991-100μg | Recombinant Murine IL-36γ, 152a.a. | 100μg | 12777.00元 |
| P5991-1mg | Recombinant Murine IL-36γ, 152a.a. | 1mg | - |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Mouse | 215257 | Q3U0P4 | E. coli | 152aa | 17.3kDa | － |
| About this protein | |
| Name | Recombinant Murine IL-36γ, 152a.a. (Recombinant Murine Interleukin-36 gamma, 152a.a.; rMuIL-36γ, 152a.a.)；Recombinant Mouse IL-36γ, 152a.a. ; 重组小鼠白细胞介素36γ，152a.a. |
| Synonyms | Interleukin-36 gamma; Interleukin 1 family, member 9; Il1f9 |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10ng/ml, corresponding to a specific activity of >1.0×105IU/mg. |
| Physical Appearance | Sterile Filtered lyophilized (freeze-dried) liquid-like powder. |
| Formulation | Lyophilized from a 0.2μm filtered solution in 1M MOPS, 10mM NaAC, pH7.6, with 2mM EDTA, 5% Trehalose, 0.02% Tween-20. |
| Endotoxin | Less than 0.1EU/μg of rMuIL-36γ, 152a.a. as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36γ is secreted when transfected into 293-T cells and it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). Furthermore, IL-36γ also can function as an agonist of NF-kappa B activation through the orphan IL-1- receptor-related protein 2. Recombinant murine IL-36γ is synthesized as a 17.3kDa, 152 amino acid (a.a.) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site. Murine to human, IL-36γ shares 53% a.a. identity. Within the family, IL-36γ shares about 25% ~ 55% a.a. sequence identity with IL-1RA, IL-1β, IL-36RA, IL-36α, IL- 37, IL-36β and IL-1F10. |
| Amino Acid Sequence | GRETPDFGEV FDLDQQVWIF RNQALVTVPR SHRVTPVSVT ILPCKYPESL EQDKGIAIYL GIQNPDKCLF CKEVNGHPTL LLKEEKILDL YHHPEPMKPF LFYHTRTGGT STFESVAFPG HYIASSKTGN PIFLTSKKGE YYNINFNLDI KS |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P5991-2μg | Recombinant Murine IL-36γ, 152a.a. | 2μg |
| P5991-10μg | Recombinant Murine IL-36γ, 152a.a. | 10μg |
| P5991-100μg | Recombinant Murine IL-36γ, 152a.a. | 100μg |
| P5991-1mg | Recombinant Murine IL-36γ, 152a.a. | 1mg |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂，此时冻干后本产品看起来仍然像是液体，属于正常现象。在冻干工艺上，海藻糖作为常用的冻干保护剂，可显著阻止蛋白质二级结构的改变，防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
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