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多肽与蛋白> 重组蛋白> 细胞因子
Recombinant Murine sTNF RI/TNFRSF1A
产品编号: P6060-1mg
产品包装:1mg
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价格: ¥ 23581.00
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产品编号 产品名称 产品包装 产品价格
P6060-5μg Recombinant Murine sTNF RI/TNFRSF1A 5μg 669.00元
P6060-100μg Recombinant Murine sTNF RI/TNFRSF1A 100μg 7363.00元
P6060-500μg Recombinant Murine sTNF RI/TNFRSF1A 500μg 12990.00元
P6060-1mg Recombinant Murine sTNF RI/TNFRSF1A 1mg -
Species Gene ID Accession Source Length MW Tag
Mouse 21937 P25118 E. coli 191aa 21.1kDa
About this Antibody
Name Recombinant Murine sTNF RI/TNFRSF1A (Recombinant Murine soluble Tumor Necrosis Factor Receptor Type I/TNFRSF1A; rMusTNF RI/TNFRSF1A);Recombinant Mouse sTNF RI/TNFRSF1A ; 重组小鼠可溶性肿瘤坏死因子受体I型/TNFRSF1A
Synonyms CD120a; TNFRSF1A; CD120a; CD120a antigen; FPF; p55; p55-R; p60; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNF-RI; TNF-R-I; TNFR-I; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
Purity >97% by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 1μg/ml, corresponding to a specific activity of >1000IU/mg in the presence of 0.1ng/ml of rMuTNF-α.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.
Endotoxin Less than 0.1EU/μg of rMusTNF RI/TNFRSF1A as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category Cytokine
Background TNF RI, also named as TNFR-I, TNF R1, TNFRSF1A, p55 and p60, belongs to the tumor necrosis factor receptor superfamily and it is encoded by the TNFRSF1A gene in human. TNF RI is a universally expressed protein and is thought to form homotrimers after ligand binding. TNF RI is one of the major receptors for the TNF-α. This receptor can activate the transcription factor NF-κB, mediate apoptosis, and function as a regulator of inflammation. Murine TNF RI shares 70%, 88%, 67%, 70% and 64%a.a. sequence identity with human, rat, canine, feline and porcine TNF RI, respectively.
Amino Acid Sequence IHPSGVTGLV PSLGDREKRD SLCPQGKYVH SKNNSICCTK CHKGTYLVSD CPSPGRDTVC RECEKGTFTA SQNYLRQCLS CKTCRKEMSQ VEISPCQADK DTVCGCKENQ FQRYLSETHF QCVDCSPCFN GTVTIPCKET QNTVCNCHAG FFLRESECVP CSHCKKNEEC MKLCLPPPLA NVTNPQDSGT A

包装清单:

产品编号 产品名称 包装
P6060-5μg Recombinant Murine sTNF RI/TNFRSF1A 5μg
P6060-20μg Recombinant Murine sTNF RI/TNFRSF1A 20μg
P6060-100μg Recombinant Murine sTNF RI/TNFRSF1A 100μg
P6060-1mg Recombinant Murine sTNF RI/TNFRSF1A 1mg
- 说明书 1份

保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明,碧云天相关产品均为冻干粉,由于微量的蛋白在冻干过程中沉积在管内,形成很薄或不可见的蛋白层,所以在打开管盖前,我们建议在离心机中约8,000-12,000g离心10-30秒,使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的,一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子,即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡,以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化。

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