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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P7064-100IU | Recombinant Bovine EK, His-tag | 100IU | 669.00元 |
| P7064-250IU | Recombinant Bovine EK, His-tag | 250IU | 1671.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Bovine | 282009 | P98072 | E. coli | 241aa | 28kDa | His |
| About this Protein | |
| Name | Recombinant Bovine EK, His-tag (Recombinant Bovine Enterokinase Light Chain, His-tag; rBoEK, His-tag)；重组牛轻链肠激酶，His标签 |
| Synonyms | Enterokinase; ENTK; PRSS7; TMPRSS15; EC 3.4.21; EC 3.4.21.9; Enterokinase; ENTKenterokinase; MGC133046; protease, serine, 7 (enterokinase); PRSS7enteropeptidase; Serine protease 7; Transmembrane protease serine 15; transmembrane protease, serine 15 |
| Purity | N/A |
| Biological Activity | N/A |
| Physical Appearance | Sterile liquid. |
| Formulation | 50mM Tris-HCl, pH8.0, 0.5M NaCl and 50% glycerol. |
| Endotoxin | Less than 1EU/μg of rBoEKL, His-tag as determined by LAL method. |
| Reconstitution | N/A |
| Category | Enzymes |
| Background | Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion. It consists of a disulfidelinked 82-140kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35-62kDa light chain which contains the catalytic subunit. Additionally, both of the chains are derived from a single precursor that is cleaved by a trypsin-like protease. EK can specially recognize the amino acid sequence DDDDK, and digest the peptide bond after the lysine residue. rEK was report to be more effective than nature EK in cleaving recombinant proteins. Furthermore, the light chain possesses the whole enzyme activity of EK. rBoEK has the highest activity than EK of other species and is used wildly in biochemical applications. rBoEK with 6X His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system. |
| Amino Acid Sequence | N/A |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P7064-100IU | Recombinant Bovine EK, His-tag | 100IU |
| P7064-250IU | Recombinant Bovine EK, His-tag | 250IU |
| P7064-1000IU | Recombinant Bovine EK, His-tag | 1000IU |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
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