产品编号 | 产品名称 | 产品包装 | 产品价格 |
P7298-500μg | Recombinant Human Galectin-1 | 500μg | 5418.00元 |
P7298-100μg | Recombinant Human Galectin-1 | 100μg | 3075.00元 |
P7298-10μg | Recombinant Human Galectin-1 | 10μg | 669.00元 |
P7298-1mg | Recombinant Human Galectin-1 | 1mg | - |
Species | Gene ID | Accession | Source | Length | MW | Tag |
Human | 3956 | P09382 | E. coli | 134aa | 14.6kDa | - |
About this Antibody | |
Name | Recombinant Human Galectin-1 (Recombinant Human Galectin-1; rHuGalectin-1);重组人半乳糖凝集素1 |
Synonyms | BHL; Galaptin; GBP; L-14; LGALS1; 14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; DKFZp686E23103; GAL1; gal-1; Gal-1; Galaptin; galaptin; galectin 1; galectin-1; GBP; HBL; HLBP14; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; Putative MAPK-activating protein PM12; S-Lac lectin 1 |
Purity | >95% by SDS-PAGE and HPLC analyses. |
Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human blood monocytes is in a concentration range of 1.0-10ng/ml. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | Lyophilized from a 0.2μm filtered solution in PBS, pH7.4, with 1mM DTT. |
Endotoxin | Less than 0.1EU/μg of rHuGalectin-1 as determined by LAL method. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
Category | Others |
Background | Human Galectin-1 also named BHL, Galaptin, GBP, L-14 and LGALS1, is belonging to the galectins family and it is encoded by the LGALS1 gene in human. Galectin-1 is expressed by the endometrial stromal cells throughout the menstrual cycle. Galectin-1 contains a single carbohydrate recognition domain through which it can bind glycans both as a monomer and as a homodimer. Dimers are non-covenantly bound and will spontaneously disassociate in low concentration. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 is thought to play a role in the immunosuppression required for a successful pregnancy. Human Galectin-1 shares 88%-90% amino acid sequence identity with rat and mouse. |
Amino Acid Sequence | ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD |
包装清单:
产品编号 | 产品名称 | 包装 |
P7298-10μg | Recombinant Human Galectin-1 | 10μg |
P7298-50μg | Recombinant Human Galectin-1 | 50μg |
P7298-100μg | Recombinant Human Galectin-1 | 100μg |
P7298-1mg | Recombinant Human Galectin-1 | 1mg |
- | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用说明:
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明,碧云天相关产品均为冻干粉,由于微量的蛋白在冻干过程中沉积在管内,形成很薄或不可见的蛋白层,所以在打开管盖前,我们建议在离心机中约8,000-12,000g离心10-30秒,使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的,一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子,即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡,以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化。