使用说明:
1. 根据样品选择最合适的膜。为获得最大回收率,超滤管的截留分子量至少小于目的蛋白分子量的1/2。
2. 加入样品,并确保超滤管盖盖好。对于水平转子样品起始体积最大为15ml,对于固定角转子样品起始体积最大为9ml。
3. 将超滤管插入50ml离心机转子中。本超滤管为双垂直过滤膜,当使用固定角转子时,调整超滤管,使得刻度印刷面在两侧(即滤膜面板在两侧),使用水平转子时没有此要求。根据样品保存条件,选择是否4℃离心。
4. 离心10-30分钟,本超滤管推荐的最高离心力为4000g,请勿超过此离心力,并注意rpm和g之间的转换。一旦达到所需的浓度,从离心机中取出超滤管并用移液器从超滤管的死体积收集器中吸取样品。
以上为主要操作步骤。赛多利斯完整使用说明书请参考碧云天网站资料:Vivaspin® Turbo 4 and 15-Technical data and operating instructions。
更多的Application Note请点击下载或参考赛多利斯官网:https://www.sartorius.com.cn
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