使用说明：
1. 固定细胞或组织切片的荧光染色：
   a. 用PBS洗涤细胞或组织切片2次。
   b. 用碧云天的免疫染色固定液(P0098)或PBS配制的3.7%甲醛溶液室温固定细胞或组织切片约10分钟。
      注意：甲醇可以破坏actin蛋白，因此不能使用含有甲醇的固定液。并且尽量使用不含甲醇的甲醛。
   c. 用碧云天的免疫染色洗涤液(P0106)或含0.1% Triton X-100的PBS洗涤2-4次，每次约5分钟。
   d. 用碧云天的免疫荧光染色二抗稀释液(P0108)或含有1-5%BSA和0.1% Triton X-100的PBS按照1:40-
      1:200的比例稀释Actin-Tracker Green，例如5μl或25μl Actin-Tracker Green用1ml稀释液稀释，稀
      释后的溶液即为Actin-Tracker Green染色工作液。注：稀释比例可以根据实际染色效果进行适当调
      整。
   e. 把Actin-Tracker Green染色工作液按照每个片子约200μl的比例滴加到片子上，室温避光孵育20-60
      分钟。为防止蒸发，孵育时最好将片子置于载玻片染色盒中，载玻片染色盒(FSR958)可以向碧云天订
      购。
   f. 用碧云天的免疫染色洗涤液(P0106)或含0.1% Triton X-100的PBS洗2-4次，每次约5分钟。
   g. 随后可以直接用荧光显微镜进行观察。为长期保存，可以在片子自然干燥后封片并于4℃避光保存，
      保存时间可长达6个月左右。
2. 对于活细胞的荧光染色：
   Phalloidin通常不具有细胞通透性，因此极少用于活细胞染色。但也有报道活细胞可被标记，可能是通
   过细胞的吞噬作用进入了某些细胞。一般对于活细胞的染色需要更多的染料。

使用本产品的文献：
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   MDA-MB-231cells.
   Tumori. 2010 Sep-Oct;96(5):751-5.
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   A pH-responsive mesoporous silica nanoparticles-based multi-drug delivery system
   forovercoming multi-drug resistance.
   Biomaterials. 2011 Oct;32(30):7711-20.
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   Protective effects of bone morphogenetic protein 7 against amyloid-beta induced
   neurotoxicity inPC12 cells.
   Neuroscience. 2011 Jun 16;184:151-63.
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   Active deformation of apoptotic intestinal epithelial cells with adhesion-restricted
   polaritycontributes to apoptotic clearance.
   Lab Invest. 2011 Mar;91(3):462-71.
5. Guo HF, Li ZS, Dong SW, Chen WJ, Deng L, Wang YF, Ying DJ.
   Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications.
   Colloids Surf B Biointerfaces. 2012 Aug 1;96:29-36.
6. Sun X, Liu X, Zhang Y, Kuang X, B Lv, J Ge.
   A simple and effective pressure culture system modified from a transwell cell culture
   system.
   Biol Res 46, 2013, 47-52.
7. Wang M, Wang Y, Chen Y, Gu H.
   Improving endothelialization on 316L stainless steel through wettability controllable
   coating by sol–gel technology.
   Applied Surface Science Volume 268, 1 March 2013, Pages 73–78.
8. Zhang W, Tang B, Huang Q, Hua Z.
   Galangin inhibits tumor growth and metastasis of B16F10 melanoma.
   J Cell Biochem. 2013 Jan;114(1):152-61.
9. Wang M, Wang Y, Chen Y, Gu H.
   Improving endothelialization on 316L stainless steel through wettability controllable
   coating by sol–gel technology.
   Applied Surface Science.Volume 268, 1 March 2013, Pages 73–78.
10.Li Z, Jiang J, Chen Y, You L, Huang Y, Tan A, Li Z, Jiang J, Niu B, Meng Z.
   PDP1 regulates energy metabolism through the IIS-TOR pathway in the red flour beetle,
   Tribolium castaneum.
   Arch Insect Biochem Physiol. 2014 Mar;85(3):127-36. doi: 10.1002/arch.21146. Epub
   2014 Jan 29.
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   TAT-LHRH conjugated low molecular weight chitosan as a gene carrier specific for
   hepatocellular carcinoma cells.
   Int J Nanomedicine. 2014 Jun 10;9:2879-89. doi: 10.2147/IJN.S61392. eCollection 2014.
12.Pi J, Li T, Liu J, Su X, Wang R, Yang F, Bai H, Jin H, Cai J.
   Detection of lipopolysaccharide induced inflammatory responses in RAW264.7 macrophages
   using atomic force microscope.
   Micron. 2014 Oct;65:1-9. doi: 10.1016/j.micron.2014.03.012. Epub 2014 Apr 1.
13.Wang J, Wei Y, Yi H, Liu Z, Sun D, Zhao H.
   Cytocompatibility of a silk fibroin tubular scaffold.
   Mater Sci Eng C Mater Biol Appl. 2014 Jan 1;34:429-36. doi: 10.1016/j.msec.2013.
   09.039. Epub 2013 Oct 5.