使用说明：
1. 加入等体积的溶液I，混匀。
   例如如DNA样品体积为100微升，则加100微升溶液I。如果等体积混合后体积较大，可以把部分样品加入
   到纯化柱内，经离心处理后，再加入剩余的样品继续处理。矿物油对于本试剂盒没有干扰。
2. 加入到DNA纯化柱内， 室温放置1分钟。
3. 最高速(16000g，约12000-14000rmp左右)离心1分钟，倒弃收集管内的液体。
   注意：这一步一定要达到16000g，较低离心速度会导致回收效率下降。
4. 在DNA纯化柱内加入700微升溶液II，室温放置1分钟。
5. 最高速离心1分钟，洗去杂质。倒弃收集管内的液体。
6. 再加入500微升溶液II，最高速离心1分钟，进一步洗去杂质。倒弃收集管内的液体。
7. 最高速再离心1分钟，除去残留液体并让残留的乙醇充分挥发。
8. 将DNA纯化柱置于1.5毫升离心管上，加入30微升溶液III至管内柱面上，放置1分钟。
   用1.5ml离心管作为收集管。溶液III需要直接加至管内柱面中央，使液体被纯化柱吸收。如果不慎将溶
   液III沾在管壁上，一定要震动管子，使液体滑落到管底，以便被纯化柱吸收。也可以用重蒸水或MiliQ
   级纯水替代溶液III，但是水的pH应不小于6.5。如需得到较高浓度的DNA，可以只加20微升溶液III，但
   产量会略有下降。放置较长时间例如3-5分钟，会对提供产量略有帮助。
9. 最高速离心1分钟，所得液体即为高纯度DNA。

使用本产品的相关论文：
1. Wen-guang Zhou, Yu-tuo Wei, Kun Huang and Ri-bo Huang.
   Cloning and expression of glycerol dehydratase gene from Klebsiella pneumoniae in
   Escherichia coli.
   Indust Microbiol. 2004, 34(2): 1-4.
2. Luan YX, Yao YG, Xie RD, Yang YM, Zhang YP, Yin WY
   Analysis of 18S rRNA gene of Octostigma sinensis (Projapygoidea: Octostigmatidae)
   supports the monophyly of Diplura.
   Pedobiologia. 2004;48:453-459.
3. Cong L, Li Y, Yu M, Ye H, Zhang X, Hu R.
   Changes of gene expressions of chymase and angiotensin2converting enzyme in heart of
   diabetic hamster.
   Chin J Diabetes. 2005; 13(6):459-462.
4. Qian YF, Wang H, Yao WB, Gao XD.
   Aqueous extract of the Chinese medicine, Danggui-Shaoyao-San, inhibits apoptosis in
   hydrogen peroxide-induced PC12 cells by preventing cytochrome c release and inactivating
   of caspase cascade.
   Cell Biol Int. 2008 Feb;32(2):304-11.
5. Jiang CY, Sheng XF, Qian M, Wang QY.
   Isolation and characterization of a heavy metal-resistant Burkholderia sp. from heavy
   metal-contaminated paddy field soil and its potential in promoting plant growth and
   heavy metal accumulation in metal-polluted soil.
   Chemosphere. 2008 May;72(2):157-64.
6. Sheng XF, Xia JJ, Jiang CY, He LY, Qian M.
   Characterization of heavy metal-resistant endophytic bacteria from rape (Brassica napus)
   roots and their potential in promoting the growth and lead accumulation of rape.
   Environ Pollut. 2008 Dec;156(3):1164-70.
7. Qiao Y, Huang Y, Qiu C, Yue X, Deng L, Wan Y, Xing J, Zhang C, Yuan S, Dong A, Xu J.
   The use of PEGylated poly [2-(N,N-dimethylamino) ethyl methacrylate] as a mucosal DNA
   delivery vector and the activation of innate immunity and improvement of HIV-1-specific
   immune responses.
   Biomaterials. 2010 Jan;31(1):115-23. Epub 2009 Sep 24.
8. Chai J, Xiong Q, Zhang P, Zheng R, Peng J, Jiang S.
   Induction of Ca2+ signal mediated apoptosis and alteration of IP3R1 and SERCA1
   expression levels by stress hormone in differentiating C2C12 myoblasts.
   Gen Comp Endocrinol. 2010 Apr 1;166(2):241-9. Epub 2009 Aug 31.
9. He LY, Zhang YF, Ma HY, Su LN, Chen ZJ, Wang QY, Qian M, Sheng XF.
   Characterization of copper-resistant bacteria and assessment of bacterial communities in
   rhizosphere soils of copper-tolerant plants.
   Applied Soil Ecology 44 (2010) 49–55.
10.Zhang YF, He LY, Chen ZJ, Wang QY, Qian M, Sheng XF.
   Characterization of ACC deaminase-producing endophytic bacteria isolated from
   copper-tolerantplants and their potential in promoting the growth and copper
   accumulation of Brassica napus.
   Chemosphere. 2011 Mar;83(1):57-62.
11.QIU H, XU ZR, SHAO XJ, ZHOU YH, XU L, WU SL
   Binding Analysis of Transcription Factor Ets-1 with the Upstream Sequence of β1,
   3-N-Acetylglucosaminyltransferase Gene.
   中国生物化学与分子生物学报，2012，28( 1) : 61 ～ 65
12.Ren H, Zhu F, Zheng C, Sun X, Wang W, Shu H
   Transcriptome analysis reveals genes related to floral development in chrysanthemum
   responsive tophotoperiods.
   Biochem Genet.2013 Feb;51(1-2):20-32.doi:10.1007/s10528-012-9541-1.Epub 2012 Sep 30.
13.Zhou W, Zhang H, Ma Y, Zhou J, Zhang Y.
   Bio-removal of cadmium by growing deep-sea bacterium Pseudoalteromonas sp. SCSE709-6.
   Extremophiles. 2013 Jun 28.
14.Zhang X, Liu W, Yang H, Tan L, Ao L, Liu J, Cao J, Cui Z.
   Inhibition of PPARα attenuates vimentin phosphorylation on Ser-83 and collapse of
   vimentin filaments during exposure of rat Sertoli cells in vitro to DBP.
   Reprod Toxicol. 2014 Oct 4. pii: S0890-6238(14)00252-4. doi: 10.1016/
   j.reprotox.2014.09.015.
15.Lin B, Lyu J, Lyu XJ, Yu HQ, Hu Z, Lam JC, Lam PK.
   Characterization of cefalexin degradation capabilities of two Pseudomonas strains
   isolated from activated sludge.
   J Hazard Mater. 2014 Jul 15. pii: S0304-3894(14)00569-X. doi: 10.1016/
   j.jhazmat.2014.06.080.
16.Chen H, Long H, Cui X, Zhou J, Xu M, Yuan G.
   Exploring the formation and recognition of an important G-quadruplex in a HIF1α promoter
   and its transcriptional inhibition by a benzo[c]phenanthridine derivative.
   J Am Chem Soc. 2014 Feb 12;136(6):2583-91. doi: 10.1021/ja412128w. Epub 2014 Feb 3.
17.Peng C, Chen J, Tang W, Liu C, Chen X.
   Kaposi's sarcoma-associated herpesvirus ORF6 gene is essential in viral lytic
   replication.
   PLoS One. 2014 Jun 9;9(6):e99542. doi: 10.1371/journal.pone.0099542. eCollection 2014.