使用说明：
1. 样品处理
   a) 对于石蜡切片：
       二甲苯中脱蜡5-10分钟。
       换用新鲜的二甲苯，再脱蜡5-10分钟。
       无水乙醇5分钟。
       90%乙醇2分钟。
       70%乙醇2分钟。
       蒸馏水2分钟。
   b) 对于冰冻切片：
       蒸馏水2分钟。
   c) 对于培养细胞：
       用4%多聚甲醛固定10分钟以上。
       蒸馏水洗涤2分钟。
       换用新鲜的蒸馏水，再洗涤2分钟。
2. 苏木素伊红(HE)染色
    对于上述处理好的样品：
    苏木素染色液染色5-10分钟(可以根据染色结果和要求调整时间)。
    浸自来水中冲洗去多余的染色液，约10分钟。
    蒸馏水再洗涤一遍(数秒钟)。
    95%乙醇5秒。
    伊红染色液染色30秒-2分钟(可以根据染色结果和要求调整时间)。
    此时，如果需要直接观察，可以用70%乙醇洗涤2次。如需脱水、透明后封片按后续步骤进行，
70%乙醇洗涤后仍可按照后续步骤进行脱水、透明和封片处理。
3. 脱水、透明、封片或进行其它染色
   a) 脱水、透明、封片：
       95%乙醇脱水2分钟。
       换用新鲜的95%乙醇再脱水2分钟。
       二甲苯透明5分钟。
       换用新鲜的二甲苯，再透明5分钟。
       用中性树胶或其它封片剂封片。
       显微镜下观察，细胞核呈蓝色，而细胞浆呈粉红色或红色。
   b) 进行其它染色：
       如果进行免疫荧光染色，或进行Hoechst等荧光染料的染色，在伊红染色液染色后：
       70%乙醇洗涤2次，每次2分钟。
       PBS或生理盐水或TBS或TBST等用于免疫染色或荧光染料染色的溶液浸泡5分钟。
       然后就可以进行免疫荧光染色或其它荧光染料的染色了。

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