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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| L06255 | MPHOSPH8基因敲除质粒 | 5µg | 999.00元 |
| L06258 | MPHOSPH8基因敲除HEK293T细胞RIPA裂解液 | 100µg | 1258.00元 |
| L06259 | MPHOSPH8基因敲除HEK293T细胞Trizol裂解液 | 500µl | 1258.00元 |
| L06256 | MPHOSPH8基因敲除慢病毒 | 10^8 TU | 6999.00元 |
| L06257 | MPHOSPH8基因敲除HEK293T细胞 | 1支/瓶 | 7999.00元 |
MPHOSPH8 Knockout Lentivirus(MPHOSPH8基因敲除慢病毒)是一种感染动物细胞后可以同时表达Cas9、目的基因sgRNA和puromycin抗性基因的慢病毒。本产品用于在动物细胞中基于CRISPR/Cas9技术敲除目的基因，并且本慢病毒中sgRNA的有效性已经通过T7EI法的验证。
本慢病毒基因序列的关键图谱信息请参考图1。本慢病毒可用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 可同时表达sgRNA、Cas9和puromycin抗性的本慢病毒其基因序列的关键图谱信息。
用于包装本慢病毒的质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。
本慢病毒用于实验时，建议同时选购无任何靶向的对照慢病毒Control Knockout Lentivirus (L00015)或靶向GFP的对照慢病毒GFP Knockout Lentivirus (L00017)。
碧云天同时提供基于CRISPR/Cas9技术的MPHOSPH8基因敲除的质粒(L06255 pLenti-MPHOSPH8-sgRNA)、慢病毒(L06256 MPHOSPH8 Knockout Lentivirus)、HEK293T细胞(L06257 MPHOSPH8 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L06258 MPHOSPH8 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L06259 MPHOSPH8 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。
MPHOSPH8基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | MPHOSPH8 | 54737 | BC003542 | NM_017520 |
| About the gene | |
| Official Symbol | MPHOSPH8 |
| Previous Symbol | - |
| Official Full Name | M-phase phosphoprotein 8 |
| Synonyms | mpp8; HSMPP8 |
| Location | 13q12.11 |
| Gene Type | protein_coding |
| Uniprot ID | Q99549 |
| Pathway/Library | Epigenetic Regulators Related Genes Library |
| Gene Summary | Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed:26022416, PubMed:28581500). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602). MPP8_HUMAN,Q99549 |
| 产品编号 | 产品名称 | 包装 |
| L06256 | MPHOSPH8 Knockout Lentivirus | 108 TU |
| — | 说明书 | 1份 |
-80℃保存，至少一年有效。
注意事项：碧云天拥有sgRNA序列的知识产权，如果需要sgRNA序列，请在订购后发送邮件向info@beyotime.com索取。sgRNA序列信息与本慢病毒，未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明来源。
对于非目录产品的CRISPR基因敲除用的慢病毒的定制，可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| L00015 | Control Knockout Lentivirus | 108 TU |
| L00017 | GFP Knockout Lentivirus | 108 TU |
| C0222 | 青霉素-链霉素溶液(100X) | 100ml |
| C0351-1ml | Polybrene (Hexadimethrine Bromide) | 1ml |
| C0351-50mg | Polybrene (Hexadimethrine Bromide) | 50mg |
| D0508S/M | 基因组编辑突变检测试剂盒 | 25/100次 |
| D7080S/M/L | T7 Endonuclease I (CRISPR等基因突变鉴定用) | 250/1250/5000U |
| ST551-10mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×1ml |
| ST551-50mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×5ml |
| ST551-250mg | Puromycin Dihydrochloride (嘌呤霉素) | 250mg |
| ST1380-500mg | Polybrene (≥94%, Reagent grade) | 500mg |
| ST1380-2g | Polybrene (≥94%, Reagent grade) | 2g |
| ST1380-10g | Polybrene (≥94%, Reagent grade) | 10g |
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