使用说明：
1. 收集细胞：
    对于贴壁细胞先用胰酶和/或EDTA消化下细胞。对于悬浮细胞，则可以直接收集细胞。把收集的细胞在
    1000-2000g离心1分钟，弃上清，用1毫升或根据细胞的量用适当细胞重悬液重新悬起细胞。
2. 台盼蓝染色：
    吸取100微升重悬的细胞到一塑料离心管内，加入100微升台盼蓝染色液(2X)，轻轻混匀，染色3分钟
    (染色3分钟时间已经足够，但染色时间可以更长一些，但不宜超过10分钟)。
3. 计数：
    吸取少量经过染色的细胞，用血细胞计数板计数。通常如果要比较精确地进行定量，每个细胞样品至少
    数500个细胞，数出蓝色细胞和细胞总数。
    细胞存活率＝(细胞总数－蓝色细胞数)/细胞总数X100%

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