After 50 consecutive passages of H9 cells using Beyotime BeyoES™ Human Pluripotent Stem Cell Medium (C0800), the cells maintain their ability for tri-lineage differentiation, as shown below.
BeyoES™ Human Pluripotent Stem Cell Medium is specifically designed for serum-free cultivation of human induced pluripotent stem cells (iPSCs) or human embryonic stem cells (hESCs) in a free-feeding layer culture system. It also promotes colony formation and expansion of stem cells.
Key Features
- Defined Composition: Free from undefined animal-derived extracellular matrix extracts, the primary components include bFGF, TGFβ, Vitamin C, insulin, transferrin, selenium, similar to Essential 8™ Medium (Gibco, A1517001) and mTeSR™1 (STEMCELL, 85850).
- Cost-Effective: When used with BeyoEmbryo™ Human Vitronectin (Beyotime, C0318/C0319), it offers excellent culture performance while significantly reducing costs.
- Stable Cell Culture: Tested with BeyoEmbryo™ Human Vitronectin coating, human embryonic stem cells remained undifferentiated after 50 passages, retaining trilineage differentiation potential and genetic stability.
- Comparable to Leading Brands: Proven to deliver results on par or superior to Competitor G (international) and Competitor S (domestic) products.
- Simplified Workflow: No need for Matrigel or feeder layers; however, it can be combined with Matrigel if required to ensure experimental outcomes.
Typical Figures
Figure 1. The cultivation effect of Beyotime BeyoES™ Human Pluripotent Stem Cell Medium (C0800) on human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) when coating with BeyoEmbryo™ Human Vitronectin (C0318/C0319).
Figure 2. The cultivation effect of Beyotime BeyoES™ Human Pluripotent Stem Cell Medium (C0800) on human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) when coating with Beyotime Matrix-Gel™ Basement Membrane Matrix (C0371/C0372).
Figure 3. Beyotime BeyoES™ Human Pluripotent Stem Cell Medium (C0800), with coating of BeyoEmbryo™ Human Vitronectin (C0318/C0319), sustains the undifferentiated status of human embryonic stem cells (hES H9).
Figure 4. After culturing for 5 passages using Beyotime BeyoES™ Human Pluripotent Stem Cell Medium (C0800) and coated with BeyoEmbryo™ Human Vitronectin (C0318/C0319), human embryonic stem cells (hES H9) maintain their ability to differentiate into all three germ layers.
Figure 5. With the use of Beyotime BeyoES™ Human Pluripotent Stem Cell Medium (C0800) and BeyoEmbryo™ Human Vitronectin (C0318/C0319), human embryonic stem cell line H9 retains its undifferentiated status and genetic stability after 50 consecutive passages.
Product Information
| Cat. No. | Product Name | Pack Size | Price |
| C0800S | BeyoES™ Human Pluripotent Stem Cell Medium | 50ml | |
| C0800M | BeyoES™ Human Pluripotent Stem Cell Medium | 500ml |
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