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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2044 | Sonic Hedgehog Protein/SHH (C-Product) Rabbit Monoclonal Antibody | 50μl | 709.00元 |
| 来源 | 用途 | 交叉反应性 | 分子量 |
| Rabbit | WB, IF, IHC, ICC, FC | H | 50.0kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
| 1:1,000-1:2,000 | - | 1:50-1:200 | 1:200-1:500 | 1:50-1:200 | 1:50-1:100 | - | - |
抗体详细信息如下:
| About this Antibody | |
| Name | Sonic Hedgehog Protein/SHH (C-Product) Rabbit Monoclonal Antibody |
| Category | Rabbit Monoclonal Antibody (RabMAb); Primary antibody |
| Isotype | IgG |
| Purification | Affinity purification |
| About the Immunogen | |
| Immunogen | Recombinant protein |
| Gene ID | 6469(Human) |
| SwissProt | Q15465(Human) |
| Synonyms | HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS |
| Category | Stem Cells & Wnt; Metabolism; Cancer Biology |
| Background | The Drosophila segment polarity gene hedgehog (hh) encodes a precursor protein which undergoes autocleavage to generate amino- and carboxy-terminal peptides. Both proteins are secreted and appear to function in embryonic and imaginal disc patterning. Several vertebrate homologs of Drosophila hh have been identified. These include Sonic hedgehog (Shh) (alternatively designated Vhh-1), Desert hedgehog (Dhh) and Indian hedgehog (Ihh). Each contain amino-terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions. Shh resembles Drosophila hh in that it is processed to generate an amino-terminal secreted peptide that is retained at or near the cell surface and a carboxy-terminal glycosylated more diffusible peptide. |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| AF2044 | Sonic Hedgehog Protein/SHH (C-Product) Rabbit Monoclonal Antibody | 50μl |
| AZ050 | Western一抗稀释液 | 50ml |
| - | 说明书 | 1份 |
保存条件:
Sonic Hedgehog Protein/SHH (C-Product) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明:
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页： http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页： http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片：
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| Fig. 1. Western blot analysis of Sonic Hedgehog Protein on different lysates using anti-Sonic Hedgehog Protein antibody at 1/1,000 dilution. Positive control: Lane 1: Hela; Lane 2: HepG2 | Fig. 2. ICC staining Sonic Hedgehog Protein in Hela cells (green).The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS. | |
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| Fig. 3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Sonic HedgehogProtein antibody. Counter stained with hematoxylin. | Fig. 4. Flow cytometric analysis of Hela cells with Sonic Hedgehog Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugatedgoat anti rabbit IgG was used as the secondary antibody. |
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