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来源
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用途
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交叉反应性
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理论分子量
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实际分子量
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Rabbit
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WB, IHC
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H, M, R
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31kDa
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17/35kDa
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WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ChIP
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ChIP-seq
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1:500-1:2000
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-
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-
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗体详细信息如下::
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About this Antibody
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Name
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Caspase 3 Rabbit Polyclonal Antibody (KO Validated)
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Category
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Rabbit Polyclonal Antibody (pAb); Primary antibody
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Isotype
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IgG
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Purification method
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Affinity purification
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Positive samples
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Jurkat
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Cellular location
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Cytoplasm
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Customer validation
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WB (Human, P. notoginseng, Mouse, Rat, Pig, Chicken)
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About the Immunogen
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Immunogen
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Recombinant fusion protein of human Caspase 3 (NP_004337.2).
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Sequence
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FHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFII
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Gene ID
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836
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Swiss Prot
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P42574
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Synonyms
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CASP3; CPP32; CPP32B; SCA-1; caspase-3
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Category
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Regulation of Apoptosis; Mitochondrial Control of Apoptosis; Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease; Death Receptor Signaling
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Background
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Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa member of the peptidase C14A family of enzymes. It is widely expressed and is an integral component of the apoptotic cascade. Caspase-3 is considered to be the major executioner caspase; that is, the primary downstream mediator of apoptotic-associated proteolysis. Active Caspase-3 is known to utilize a Cys residue to cleave multiple substrates, including PARP, proIL 16, PKC-gamma & -δ, procaspases 6, 7 and 9, and beta catenin. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein. Normally, it is an inactive, cytosolic homodimer, but following an upstream signal that activates processing proteases, procaspase-3 undergoes proteolytic cleavage. This generates an N-terminal 175 aa p20/20 kDa subunit plus a 102 aa C-terminal p12/12 kDa subunit, followed by further processing of the p20 subunit at Asp28 to generate a final p17 subunit (aa 29 175) The p17 and p12 subunits noncovalently heterodimerize, and subsequently associate with another p17/p12 heterodimer to form an active antiparallel homodimer. The p17 subunit contains the enzyme active site (aa 161 165), with an embedded catalytic Cys which is normally nitrosylated and inactive. Full activation requires both proteolytic processing and Cys163 denitrosylation. Multiple proteases can use Caspase-3 as a substrate including Caspase-6, -8, and -10, granzyme B, and Caspase-3 itself.
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包装清单：
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产品编号
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产品名称
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包装
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AF5132
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Caspase 3 Rabbit Polyclonal Antibody (KO Validated)
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50μl
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AZ050
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Western一抗稀释液
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50ml
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—
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说明书
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1份
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保存条件：
Caspase 3 Rabbit Polyclonal Antibody (KO Validated) -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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