碧云天生物技术-Western一抗二抗去除液(强碱性)(P0025-250ml)

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Western一抗二抗去除液(强碱性)

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产品编号: P0025-250ml

产品包装:250ml

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250ml 1L

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价格: ¥ 188.00

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产品编号	产品名称	产品包装	产品价格
P0025-250ml	Western一抗二抗去除液(强碱性)	250ml	188.00元
P0025-1L	Western一抗二抗去除液(强碱性)	1L	568.00元

      Western一抗二抗去除液(Stripping buffer)，用于Western中转移了蛋白的膜的重复利用。在Western中完成了一抗二抗结合和后续的化学发光检测后，有时还需要检测tubulin、actin等表达量相对稳定的蛋白作为参照，或检测其它蛋白进行比较。通过使用一抗二抗去除液，充分去除一抗二抗，可以非常方便地重新利用使用过的膜检测其它蛋白。和重新跑一个SDS-PAGE胶相比，不仅省时省力，而且可以消除重新上样而带来的误差，使可比性更强。
      使用Western一抗二抗去除液多次重复使用同一张膜，会导致蛋白信号的减弱。但是，本Western一抗二抗去除液，经过多个抗体的检测试验，通常可以重复利用膜3-5次。
      使用本试剂只需大约15-20分钟即可实现蛋白膜的重复使用，然后可以进行封闭等后续的Western操作。
      碧云天生产的不同的Western一抗二抗去除液主要在去除结合的一抗二抗的原理方面略有不同。分别依靠去垢剂、酸性、碱性、还原剂、螯合剂、盐浓度以及一些特殊试剂等的不同组合来解除一抗二抗的结合，同时又不会导致转移到膜上的蛋白的损失。
      关于Western一抗二抗去除液的选择，一方面可以参考碧云天的相关网页：
http://www.beyotime.com/support/stripping-buffer.htm；另一方面也需要通过一些预实验来摸索最佳的适合您实验条
件的Western一抗二抗去除液。
包装清单：

产品编号	产品名称	包装
P0025-250ml	Western一抗二抗去除液(强碱性)	250ml
P0025-1L	Western一抗二抗去除液(强碱性)	500mlx2
—	说明书	1份

保存条件：
      室温保存，一年有效。
注意事项：
      使用辣根过氧化物酶(HRP)，任何一次封闭(blocking)都应当使用5%脱脂牛奶，如果使用碱性磷酸酯酶，任何一次封闭都应当使用酪蛋白(casein)。
      为取得最佳效果，推荐使用PVDF膜。但硝酸纤维素膜也可以使用本Western一抗二抗去除液。
      使用ECL、BeyoECL等类似的化学发光试剂进行的Western检测适用本试剂。使用非化学发光试剂进行的Western检测，例如DAB，NBT/BCIP，不适用于本试剂。
      本Western一抗二抗去除液有轻微腐蚀性，使用时请注意防护。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
      为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 在完成Western化学发光检测后，蒸馏水中漂洗5分钟。
2. 弃蒸馏水，加入适量的Western一抗二抗去除液(强碱性)，至少需把膜完全覆盖。在摇床上漂洗5分钟。
3. 弃Western一抗二抗去除液(强碱性)，加入蒸馏水漂洗一次。然后再加入足量蒸馏水，在摇床上漂洗5分钟。
4. 进行封闭(blocking)等Western的后续操作。

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