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来源
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用途
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交叉反应性
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理论分子量
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实际分子量
|
|
Rabbit
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WB, IP, IF
|
H, M, R
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46/48kDa
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46kDa
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WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
|
WB
|
IP
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IF
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IHC
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ICC
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FC
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ChIP
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ChIP-seq
|
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1:500-1:1000
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1:50-1:100
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1:50-1:100
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-
|
-
|
-
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-
|
-
|
抗体详细信息如下::
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About this Antibody
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Name
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GSK3β Rabbit Polyclonal Antibody (KO Validated)
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Category
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Rabbit Polyclonal Antibody (pAb); Primary antibody
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Isotype
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IgG
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Purification method
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Affinity purification
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Positive samples
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LO2, HeLa, 22Rv1, U-251MG
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|
Cellular location
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Cell membrane, Cytoplasm, Nucleus
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Customer validation
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WB (Rat); IF (Rat)
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About the Immunogen
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Immunogen
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A synthetic synthetic peptide of human GSK3B (NP_001139628.1).
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Sequence
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PWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST
|
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Gene ID
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2932
|
|
Swiss Prot
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P49841
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|
Synonyms
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GSK3B; glycogen synthase kinase-3 beta
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|
Category
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Wnt/β-Catenin Signaling; PI3K/Akt Signaling; Insulin Signaling; NF-κB Signaling
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|
Background
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Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin. GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β. In contrast, the phosphorylation of tyrosine 216 of GSK-3β or tyrosine 279 of GSK-3α in the catalytic domain increase this kinase activity to promote cell death under the absence of growth factors. GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development. GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization.
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包装清单：
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产品编号
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产品名称
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包装
|
|
AF5174
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GSK3β Rabbit Polyclonal Antibody (KO Validated)
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50μl
|
|
AZ050
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Western一抗稀释液
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50ml
|
|
—
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说明书
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1份
|
保存条件：
GSK3β Rabbit Polyclonal Antibody (KO Validated) -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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