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来源
|
用途
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交叉反应性
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理论分子量
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实际分子量
|
|
Rabbit
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WB, IP, IF, IHC
|
H
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35/55/69kDa
|
70kDa
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WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
|
IP
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IF
|
IHC
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ICC
|
FC
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ChIP
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ChIP-seq
|
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1:500-1:2000
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1:50-1:100
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
|
抗体详细信息如下:
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About this Antibody
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Name
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Phospho-ZAP70 (Tyr319) Rabbit Polyclonal Antibody
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Category
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Rabbit Polyclonal Antibody (pAb); Primary antibody
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|
Isotype
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IgG
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|
Purification method
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Affinity purification
|
|
Positive samples
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Jurkat, Preincubated with blocking peptide
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|
Cellular location
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Cell membrane, Cytoplasm, Peripheral membrane protein
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|
Customer validation
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-
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About the Immunogen
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Immunogen
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A synthetic phosphorylated peptide around Y319 of human ZAP70 (NP_001070.2).
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Sequence
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SPYSD
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Gene ID
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7535
|
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Swiss Prot
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P43403
|
|
Synonyms
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ZAP70; ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70; tyrosine-protein kinase ZAP-70
|
|
Category
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PI3K/Akt Signaling; ErbB/HER Signaling; Protein Tyrosine Kinase Signaling; T-Cell/B-Cell Activation
|
|
Background
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The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia.
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包装清单：
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产品编号
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产品名称
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包装
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|
AF5968
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Phospho-ZAP70 (Tyr319) Rabbit Polyclonal Antibody
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50μl
|
|
AZ050
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Western一抗稀释液
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50ml
|
|
—
|
说明书
|
1份
|
保存条件：
Phospho-ZAP70 (Tyr319) Rabbit Polyclonal Antibody -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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