来源
|
用途
|
交叉反应性
|
理论分子量
|
实际分子量
|
Rabbit
|
WB, IF
|
H, M, R
|
46kDa
|
70kDa
|
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ChIP
|
ChIP-seq
|
1:500-1:1000
|
-
|
1:20-1:100
|
-
|
-
|
-
|
-
|
-
|
抗体详细信息如下:
About this Antibody
|
Name
|
E2F1 Rabbit Polyclonal Antibody
|
Category
|
Rabbit Polyclonal Antibody (pAb); Primary antibody
|
Isotype
|
IgG
|
Purification method
|
Affinity purification
|
Positive samples
|
A-431, BxPC-3, U-87MG, NIH/3T3
|
Cellular location
|
Nucleus
|
Customer validation
|
-
|
About the Immunogen
|
Immunogen
|
A synthetic peptide of human E2F1
|
Sequence
|
-
|
Gene ID
|
1869
|
Swiss Prot
|
Q01094
|
Synonyms
|
E2F1; E2F-1; RBAP1; RBBP3; RBP3; transcription factor E2F1
|
Category
|
G1/S Checkpoint; ErbB/HER Signaling
|
Background
|
The E2F transcription factors are essential for regulation of the cell cycle. Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit. Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control. E2F-1 has oncogenic properties in vivo and in vitro. E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms. E2F-1 is stress-responsive, and is regulated by a PI3-kinase-like kinase family such as the ATM/ATR kinases.
|
包装清单:
产品编号
|
产品名称
|
包装
|
AF6756
|
E2F1 Rabbit Polyclonal Antibody
|
50μl
|
AZ050
|
Western一抗稀释液
|
50ml
|
—
|
说明书
|
1份
|
保存条件:
E2F1 Rabbit Polyclonal Antibody -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。