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来源
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用途
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交叉反应性
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理论分子量
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实际分子量
|
|
Rabbit
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WB, IP, IF
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H, M, R
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138/153/154kDa
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170kDa
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WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
|
WB
|
IP
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IF
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IHC
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ICC
|
FC
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ChIP
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ChIP-seq
|
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1:500-1:2000
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1:20-1:50
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1:50-1:200
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-
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-
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-
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-
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-
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抗体详细信息如下::
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About this Antibody
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Name
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RAD50 Rabbit Polyclonal Antibody
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Category
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Rabbit Polyclonal Antibody (pAb); Primary antibody
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Isotype
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IgG
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Purification method
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Affinity purification
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Positive samples
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A-549, MCF7, 293T, HepG2, NIH/3T3, Mouse testis, Mouse spleen, Mouse thymus, Rat testis
|
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Cellular location
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Chromosome, Nucleus, telomere
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Customer validation
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WB (Human)
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|
About the Immunogen
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Immunogen
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A synthetic peptide of human RAD50 (NP_005723.2).
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Sequence
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MSRIEKMSILGVRSFGIEDKDKQIITFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDPKVAQETDVRAQIRLQFRDVNGELIAVQRSM
|
|
Gene ID
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10111
|
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Swiss Prot
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Q92878
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Synonyms
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RAD50; NBSLD; RAD502; hRad50; DNA repair protein RAD50
|
|
Category
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G2M/DNA Damage Checkpoint; DNA Damage and Repair
|
|
Background
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The DNA repair protein Rad50 is a member of the structural maintenance of chromosomes family (SMC) and plays an important role in cell cycle checkpoint signaling and double-strand break repair in response to DNA damage. Rad50 forms a complex with Mre11 and Nbs1 that becomes activated in response to DNA damage. In normal human cells, the MRN complex acts to tether linear DNA molecules, providing a flexible link between DNA ends. Genomic instability and cancer have been shown to develop in cells with genetic mutations affecting the proteins in the MRN complex. ATM-dependent phosphorylation of Rad50 at Ser635 in response to DNA damage is important in regulating downstream signaling, DNA repair and checkpoint control.
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包装清单：
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产品编号
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产品名称
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包装
|
|
AF7857
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RAD50 Rabbit Polyclonal Antibody
|
50μl
|
|
AZ050
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Western一抗稀释液
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50ml
|
|
—
|
说明书
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1份
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保存条件：
RAD50 Rabbit Polyclonal Antibody -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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