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来源
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用途
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交叉反应性
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理论分子量
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实际分子量
|
|
Rabbit
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WB, IF
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H, M, R
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37kDa
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25-80kDa
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WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ChIP
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ChIP-seq
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1:500-1:2000
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-
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1:50-1:100
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-
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-
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-
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-
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-
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抗体详细信息如下:
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About this Antibody
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Name
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SLAMF7/CD319/CRACC Rabbit Polyclonal Antibody
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Category
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Rabbit Polyclonal Antibody (pAb); Primary antibody
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Isotype
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IgG
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Purification method
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Affinity purification
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Positive samples
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BxPC-3, HL-60, A-549, SKOV3, BT-474, Mouse spleen, Mouse lung, Rat testis
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Cellular location
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Membrane, Single-pass type I membrane protein
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|
Customer validation
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-
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|
About the Immunogen
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Immunogen
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Recombinant fusion protein of human SLAMF7 (NP_067004.3).
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Sequence
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SGPVKELVGSVGGAVTFPLKSKVKQVDSIVWTFNTTPLVTIQPEGGTIIVTQNRNRERVDFPDGGYSLKLSKLKKNDSGIYYVGIYSSSLQQPSTQEYVLHVYEHLSKPKVTMGLQSNKNGTCVTNLTCCMEHGEEDVIYTWKALGQAANESHNGSILPISWRWGESDMTFICVARNPVSRNFSSPILARKLCEGAADDPDSSM
|
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Gene ID
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57823
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Swiss Prot
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Q9NQ25
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Synonyms
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SLAMF7; 19A; CD319; CRACC; CS1; SLAM family member 7
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Category
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T-Cell/B-Cell Activation
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Background
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SLAMF7/CD319/CRACC (also known as CS1) is a member of the signaling lymphocytic activation molecule (SLAM) family. It is a single-pass type l transmembrane glycoprotein expressed on NK cells, subsets of mature dendritic cells, activated B and T lymphocytes, but not in promyelocytic B or T cell lines. Expression of this protein has been detected in the spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung, and trachea. Homophilic interactions of SLAMF7/CD319/CRACC modulate the activity and differentiation of immune cells. SLAMF7/CD319/CRACC may function as an inhibitory or activating receptor in immune cells depending on cellular context and availability of adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. In the presence of SH2D1B/EAT-2, SLAMF7/CD319/CRACC activates NK cells and B cells. T cells lack SH2D1B/EAT-2 expression, and therefore SLAMF7/CD319/CRACC acts as an inhibitory receptor. In LPS-activated monocytes, SLAMF7/CD319/CRACC negatively regulates production of proinflammatory cytokines. SLAMF7/CD319/CRACC is upregulated in multiple myeloma and is implicated in the uncontrolled proliferation of these cells, and thus has become the target for therapeutic intervention. Seven isoforms of SLAMF7/CD319/CRACC produced by alternative splicing have been identified.
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包装清单：
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产品编号
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产品名称
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包装
|
|
AF7989
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SLAMF7/CD319/CRACC Rabbit Polyclonal Antibody
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50μl
|
|
AZ050
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Western一抗稀释液
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50ml
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|
—
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说明书
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1份
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保存条件：
SLAMF7/CD319/CRACC Rabbit Polyclonal Antibody -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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