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来源
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用途
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交叉反应性
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理论分子量
|
实际分子量
|
|
Rabbit
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WB, IF
|
H, M, R
|
94kDa
|
112kDa
|
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
|
WB
|
IP
|
IF
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IHC
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ICC
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FC
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ChIP
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ChIP-seq
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1:500-1:2000
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-
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1:50-1:200
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-
|
-
|
-
|
-
|
-
|
抗体详细信息如下::
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About this Antibody
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Name
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TRPV1 Rabbit Polyclonal Antibody
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Category
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Rabbit Polyclonal Antibody (pAb); Primary antibody
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|
Isotype
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IgG
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|
Purification method
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Affinity purification
|
|
Positive samples
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A-431, U-87MG, BxPC-3, Mouse pancreas, Mouse brain, Mouse skin, Rat brain, Rat skin
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|
Cellular location
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Cell junction, Cell membrane, Cell projection, Multi-pass membrane protein, dendritic spine membrane, postsynaptic cell membrane, synapse
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|
Customer validation
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-
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|
About the Immunogen
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|
Immunogen
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A synthetic peptide of human TRPV1 (NP_061197.4).
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|
Sequence
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IAYSSSETPNRHDMLLVEPLNRLLQDKWDRFVKRIFYFNFLVYCLYMIIFTMAAYYRPVDGLPPFKMEKTGDYFRVTGEILSVLGGVYFFFRGIQYFLQRR
|
|
Gene ID
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7442
|
|
Swiss Prot
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Q8NER1
|
|
Synonyms
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TRPV1; VR1; transient receptor potential cation channel subfamily V member 1; Vanilloid Receptor 1; Capsaicin Receptor
|
|
Category
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Neuronal Signaling
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|
Background
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VR1, also known as TRPV1 (Transient Receptor Potential Vanilloid 1) and capsaicin receptor, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. It contributes to normal pain and temperature sensation and also has a “sensory-effector” function. TRPV1 is a 115 kDa integral membrane ion channel that has six transmembrane domains and contains intracellular N-and C-termini. By alternative splicing, at least three VR1 isoforms are known. The sequence of residues 4-21 of rat VR1 is 78% and 28% identical to that of mouse and human VR1, respectively.
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包装清单：
|
产品编号
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产品名称
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包装
|
|
AF8250
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TRPV1 Rabbit Polyclonal Antibody
|
50μl
|
|
AZ050
|
Western一抗稀释液
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50ml
|
|
—
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说明书
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1份
|
保存条件：
TRPV1 Rabbit Polyclonal Antibody -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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