|
来源
|
用途
|
交叉反应性
|
理论分子量
|
实际分子量
|
|
Rabbit
|
WB, IHC
|
H, M, R
|
28/30/86kDa
|
110kDa
|
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ChIP
|
ChIP-seq
|
|
1:500-1:2000
|
-
|
-
|
1:50-1:200
|
1:50-1:200
|
-
|
-
|
-
|
抗体详细信息如下::
|
About this Antibody
|
|
Name
|
TSHR Rabbit Polyclonal Antibody
|
|
Category
|
Rabbit Polyclonal Antibody (pAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification method
|
Affinity purification
|
|
Positive samples
|
HeLa, SKOV3, OVCAR3, mouse ovary, mouse thymus, mouse eye, rat ovary, rat thymus, rat eye
|
|
Cellular location
|
Cell membrane, Multi-pass membrane protein
|
|
Customer validation
|
IF (rat)
|
|
About the Immunogen
|
|
Immunogen
|
Recombinant fusion protein of human TSHR (NP_001018046.1).
|
|
Sequence
|
GMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLLPLGRKSLSFETQKAPRSSMPS
|
|
Gene ID
|
7253
|
|
Swiss Prot
|
P16473
|
|
Synonyms
|
TSHR; CHNG1; LGR3; hTSHR-I; thyrotropin receptor; Thyroid Stimulating Hormone Receptor
|
|
Category
|
Endocrinology & Hormones
|
|
Background
|
Thyroid stimulating hormone receptor (TSHR) is an approximately 110-120 kDa glycosylated receptor for the endocrine hormone thyrotropin (TSH). TSH consists of a common alpha subunit which is also a subunit of luteinizing hormone (LH), follicle stimulating hormone (FSH), and chorionic gonadotropin (CH), plus a beta subunit which is unique to TSH. It is produced by the anterior pituitary and triggers the thyroid gland to release thyroxine (T4). T4 is then converted to triiodothyronine (T3) which exerts wide-ranging effects on growth and metabolism. TSHR additionally binds to the related hormone thyrostimulin which is composed of different alpha and beta subunits. TSHR is the dominant target of autoreactive antibodies in Graves’ disease but is a more rare target in Hashimoto’s thyroiditis. Human TSHR consists of a 393 amino acid (aa) N-terminal extracellular domain (ECD) with 7 tandem leucine-rich repeats, a 7-transmembrane segment region, and an 82 aa C-terminal cytoplasmic tail. Within the N-terminal ECD, human TSHR shares 87% aa sequence identity with mouse and rat TSHR. Alternative splicing generates soluble isoforms that are substituted and truncated following the fifth LRR. TSHR is primarily expressed by epithelial cells of the thyroid (thyrocytes), although it has also been detected in multiple non-endocrine tissues. TSHR is expressed as a disulfide linked heterodimer; it is proteolytically cleaved, and the C-terminal fragment is subsequently trimmed at its N-terminus. The 53 kDa alpha domain can be released by disulfide reduction at the cell surface and retains the ability to bind TSH. The agonistic anti-TSHR antibodies produced in Graves’ disease preferentially recognize the free alpha subunit over the heterodimeric receptor. The activation of TSHR in brown adipose tissue can trigger the up-regulation of UCP-1 as well as thermogenesis.
|
包装清单：
|
产品编号
|
产品名称
|
包装
|
|
AF8265
|
TSHR Rabbit Polyclonal Antibody
|
50μl
|
|
AZ050
|
Western一抗稀释液
|
50ml
|
|
—
|
说明书
|
1份
|
保存条件：
TSHR Rabbit Polyclonal Antibody -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
微信在线咨询
微信在线咨询
