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来源
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用途
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交叉反应性
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理论分子量
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实际分子量
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Rabbit
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WB, IF
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H, M
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39kDa
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39kDa
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WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ChIP
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ChIP-seq
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1:500-1:2000
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-
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1:10-1:100
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-
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-
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-
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-
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-
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抗体详细信息如下:
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About this Antibody
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Name
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WNT7A Rabbit Polyclonal Antibody
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Category
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Rabbit Polyclonal Antibody (pAb); Primary antibody
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Isotype
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IgG
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Purification method
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Affinity purification
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Positive samples
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Mouse kidney
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Cellular location
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Secreted, extracellular matrix, extracellular space
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Customer validation
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-
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About the Immunogen
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Immunogen
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Recombinant fusion protein of human WNT7A (NP_004616.2).
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Sequence
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LGASIICNKIPGLAPRQRAICQSRPDAIIVIGEGSQMGLDECQFQFRNGRWNCSALGERTVFGKELKVGSREAAFTYAIIAAGVAHAITAACTQGNLSDCGCDKEKQGQYHRDEGWKWGGCSADIRYGIGFAKVFVDAR
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Gene ID
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7476
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Swiss Prot
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O00755
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Synonyms
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WNT7A; protein Wnt-7a
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Category
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Wnt/β-Catenin Signaling; ESC Pluripotency and Differentiation; Microtubule Dynamics; mTOR Signaling
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Background
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Human Wnt-7a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes. Wnts bind to receptors of the Frizzled family in conjunction with low-density lipoprotein receptor-related proteins (LRPs). Downstream effects of Wnt signaling occur through activation of one of three different intracellular pathways: the canonical Wnt pathway, Wnt/Ca2+ pathway, and planar cell polarity. The highly transforming Wnts, including Wnt-1, -3, -3a, -7a and -8 activate the canonical pathway, which regulates beta catenin-mediated gene expression. Human Wnt-7a is a 48 kDa secreted glycoprotein containing 24 cysteine residues that is expressed by epithelial and epithelially-derived cells of the placenta, kidney, testis, uterus, fetal lung and fetal and adult brain. Palmitate modification of a cysteine residue has been shown for Wnt-3a; this site is conserved on all Wnts and is Cys73 on Wnt7a. When modified, increased hydrophobicity and activity is expected. Human Wnt-7a shows 97% aa identity with mouse, rat and dog Wnt-7a and 92% aa identity with chicken Wnt-7a. During development, Wnt7a is expressed by the dorsal ectoderm and drives expression of homeodomain transcription factors that control effectors important in patterning and cell fates in adjacent mesenchyme. When Wnt-7a is deleted, mice show disruption of dorsalization and anterior/posterior patterning during limb development and abnormalities in the reproductive tract. Wnt-7a is frequently downregulated in leukemia and lung cancers, potentially affecting homeobox (HOX) gene expression, differentiation state and growth control. Roles for Wnt-7a have also been shown during formation of neural synapses, response of the uterus to estrogen and inflammatory cartilage destruction.
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包装清单：
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产品编号
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产品名称
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包装
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AF8361
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WNT7A Rabbit Polyclonal Antibody
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50μl
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AZ050
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Western一抗稀释液
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50ml
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—
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说明书
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1份
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保存条件：
WNT7A Rabbit Polyclonal Antibody -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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