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抗体> 细胞骨架/细胞外基质> Cytoskeletal Signaling
α-Smooth Muscle Actin Rabbit Monoclonal Antibody
产品编号: AG8004
产品包装:50μl
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价格: ¥ 456.00
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产品编号 产品名称 产品包装 产品价格
AG8004 α-Smooth Muscle Actin Rabbit Monoclonal Antibody 50μl 456.00元
来源 用途 交叉反应性 理论分子量 实际分子量
Rabbit WB, IHC, FC, IF/ICC H, M, R, B, Dg, Mk, Pg, Z 42kDa 42kDa

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.

H, Human; M, Mouse; R, Rat; Ar, Arabidopsis; B, Bovine; C, Chicken; Cerastium, Ce; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Ph, Pharbitis; Pg, Pig; Rb, Rabbit; Rc, Rice; S, Sheep; Z, Zebrafish; All, all species expected.

配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ChIP-seq
1:1,000-1:2,000 - 1:80-1:200 1:2,000-1:4,000 1:80-1:200 1:20-1:100 - -

抗体详细信息如下:

About this Antibody
Name α-Smooth Muscle Actin Rabbit Monoclonal Antibody
Category Recombinant Rabbit Monoclonal Antibody (mAb); Primary antibody; 内参抗体
Isotype IgG
Purification method Protein A affinity purification
Positive samples -
Cellular location Cytoplasm
Customer validation -
About the Immunogen
Immunogen A synthetic peptide corresponding to residues on the N-terminus of human Alpha Actin 2 was used as an immunogen.
Sequence -
Gene ID 59
SwissProt P62736
Synonyms Actin, aortic smooth muscle, Alpha-actin-2, ACTA2, ACTSA, ACTVS
Category Cytoskeletal Signaling; Regulation of Apoptosis
Background Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells.
包装清单:
产品编号 产品名称 包装
AG8004 α-Smooth Muscle Actin Rabbit Monoclonal Antibody 50µl
AZ050 Western一抗稀释液 50ml
说明书 1份
保存条件:

α-Smooth Muscle Actin Rabbit Monoclonal Antibody -20℃保存,需避免反复冻融。Western一抗稀释液-20℃或4℃保存,一年有效,Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。

注意事项:

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。

回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000×g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。

提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
请根据抗体的实际用途选择相应的使用方法。
1.Western检测:
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的一抗,4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页:http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片。
All lanes: Anti-α-Smooth Muscle Actin (Alpha Actin 2) antibody at 1:2,000 dilution Predicted MW: 42 kDa Observed MW: 42 kDa Lane 1: Mu Brain Lane 2: Rat Kidney Lane 3: Rat Liver Lysate at 10 μg per lane 2nd Ab: GAR HRP(H+L) 1:10,000 Exposure: 120s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of placenta labelling Alpha Actin 2 with Alpha Actin 2 Rabbit Monoclonal Antibody at 1:2,000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Alpha Actin 2 Rabbit Monoclonal Antibody staining Alpha Actin 2 in HeLa cells by IF/ICC. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:200) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1:500).
Overlay histogram showing HeLa cells stained with Alpha Actin 2 Rabbit Monoclonal Antibody (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% Triton X-100 for 15 min. The cells were then incubated in the antibody (Alpha Actin 2 Rabbit Monoclonal Antibody , 1:100 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at 4°C. Unlabelled sample (Green) was used as a control.
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