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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AG8004 | α-Smooth Muscle Actin Rabbit Monoclonal Antibody | 50μl | 456.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Rabbit | WB, IHC, FC, IF/ICC | H, M, R, B, Dg, Mk, Pg, Z | 42kDa | 42kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; Ar, Arabidopsis; B, Bovine; C, Chicken; Cerastium, Ce; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Ph, Pharbitis; Pg, Pig; Rb, Rabbit; Rc, Rice; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:1,000-1:2,000 | - | 1:80-1:200 | 1:2,000-1:4,000 | 1:80-1:200 | 1:20-1:100 | - | - |
抗体详细信息如下:
| About this Antibody | |
| Name | α-Smooth Muscle Actin Rabbit Monoclonal Antibody |
| Category | Recombinant Rabbit Monoclonal Antibody (mAb); Primary antibody; 内参抗体 |
| Isotype | IgG |
| Purification method | Protein A affinity purification |
| Positive samples | - |
| Cellular location | Cytoplasm |
| Customer validation | - |
| About the Immunogen | |
| Immunogen | A synthetic peptide corresponding to residues on the N-terminus of human Alpha Actin 2 was used as an immunogen. |
| Sequence | - |
| Gene ID | 59 |
| SwissProt | P62736 |
| Synonyms | Actin, aortic smooth muscle, Alpha-actin-2, ACTA2, ACTSA, ACTVS |
| Category | Cytoskeletal Signaling; Regulation of Apoptosis |
| Background | Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells. |
| 产品编号 | 产品名称 | 包装 |
| AG8004 | α-Smooth Muscle Actin Rabbit Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
α-Smooth Muscle Actin Rabbit Monoclonal Antibody -20℃保存，需避免反复冻融。Western一抗稀释液-20℃或4℃保存，一年有效，Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000×g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
All lanes: Anti-α-Smooth Muscle Actin (Alpha Actin 2) antibody at 1:2,000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
Lane 1: Mu Brain
Lane 2: Rat Kidney
Lane 3: Rat Liver
Lysate at 10 μg per lane
2nd Ab:
GAR HRP(H+L) 1:10,000
Exposure: 120s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of placenta labelling Alpha Actin 2 with Alpha Actin 2 Rabbit Monoclonal Antibody at 1:2,000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Alpha Actin 2 Rabbit Monoclonal Antibody staining Alpha Actin 2 in HeLa cells by IF/ICC. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:200) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1:500).
Overlay histogram showing HeLa cells stained with Alpha Actin 2 Rabbit Monoclonal Antibody (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% Triton X-100 for 15 min. The cells were then incubated in the antibody (Alpha Actin 2 Rabbit Monoclonal Antibody , 1:100 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at 4°C. Unlabelled sample (Green) was used as a control.
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