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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AG8555 | BeyoIHC™ N-Cadherin Rabbit Monoclonal Antibody | 50μl | 826.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Rabbit | IHC | Human | 100kDa | - |
IHC-P, Immunohistochemistry-paraffin; IHC-Fr, Immunohistochemistry-frozen; IF, Immunofluorescence;
ICC, Immunocytochemistry; WB, Western blot; FC, Flow Cytometry.
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| IHC-P | IHC-Fr | IF/ICC | WB | FC |
| 1:100-1:200 | N/D (not determined) | N/D | N/D | N/D |
BeyoIHC™系列抗体是经过测试适用于免疫组织化学(Immunohistochemistry, IHC)的高品质抗体，也称病理抗体，很大一部分为重组兔单克隆抗体(Recombinant Rabbit Monoclonal Antibody)，通过先进的兔单克隆抗体技术平台研发并生产，无需获取动物的脾脏，仅需分离抗原特异性B细胞并通过基因工程方法克隆抗体基因，然后在哺乳动物细胞中重组表达并最终纯化获得[1-2]。
BeyoIHC™抗体灵敏度更高，背景更低，染色更清晰，定位更精准。BeyoIHC™系列抗体通过反复的筛选和测试，确保灵敏度高、背景低、染色效果好[3]。
BeyoIHC™抗体特异性更强，染色结果更为准确。BeyoIHC™系列抗体大部分都是精心筛选的高效价单克隆抗体，是由单个B细胞淋巴瘤表达，只针对一个抗原表位发生特定的免疫反应，应用于免疫组化时特异性更强，染色结果更为准确[3]。
BeyoIHC™抗体一致性更好，批次间差异更小，结果重复性更好。BeyoIHC™系列抗体很大一部分是重组抗体，具有确定的抗体序列，使用不含任何动物源性物质培养液的哺乳细胞表达体系，生产的可重复性、一致性高，批次间差异小，可避免杂交瘤细胞制备过程中的基因丢失、基因突变和细胞株漂移等问题，所制备的重组抗体的重复性更好[3]。
BeyoIHC™系列抗体主要用于福尔马林固定石蜡包埋(Formalin fixed paraffin embedded, FFPE)样品的免疫组织化学染色(Immunohistochemistry-paraffin or Immunohistochemistry paraffin-embedded, IHC-P)。对于冰冻切片免疫组织化学(Immunohistochemistry frozen sections, IHC-Fr)、细胞免疫荧光(Immunofluorescence, IF)或免疫细胞化学(Immunocytochemistry, ICC)未经测试(Not determined)。同时，BeyoIHC™系列抗体可识别人的相应蛋白，对于小鼠等其它种属很大一部分未经测试。对于未经测试的应用及种属样品，理论上有可能是可以检测的，但须用户自行测试。
BeyoIHC™系列抗体配套提供了免疫染色一抗稀释液，可以用于IHC检测或其它适当用途时的一抗稀释。为进一步提高信噪比，推荐使用QuickBlock™免疫染色一抗稀释液(P0262)、SignalUp™免疫染色一抗稀释液(P0277)用于IHC检测。
抗体详细信息如下:
| About this Antibody | |
| Name | BeyoIHC™ N-Cadherin Rabbit Monoclonal Antibody |
| Category | Recombinant Rabbit Monoclonal Antibody (mAb); Primary antibody; IHC抗体 |
| Isotype | IgG |
| Clone number | BA1230 |
| Purification method | Protein A affinity purification |
| Positive samples | - |
| Cellular location | Membrane & Cytoplasm |
| Customer validation | - |
| Epitope retrieval | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0). |
| About the Immunogen | |
| Immunogen | Synthetic peptide corresponding to residues within CTerm of N-Cadherin was used as an immunogen. |
| Sequence | - |
| Gene ID | 1000 |
| SwissProt | P19022 |
| Synonyms | ACOGS; ARVD14; CD325; CDHN; CDw325; NCAD; CDH2 |
| Category | Adherens Junction Dynamics; Wnt/βCatenin Signaling |
| Background | Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton. While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking. Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers. Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis. Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers. |
| 产品编号 | 产品名称 | 包装 |
| AG8555 | BeyoIHC™ N-Cadherin Rabbit Monoclonal Antibody | 50µl |
| AZ060 | 免疫染色一抗稀释液 | 10ml |
| — | 说明书 | 1份 |
-20℃保存，一年有效。免疫染色一抗稀释液也可以4℃保存，长期不使用推荐-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：如果本抗体用于免疫组化(IHC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常可以重复使用1-5次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000×g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的免疫染色一抗稀释液也可以用于Western blot (WB)等适当用途。如果希望获得最佳的检测效果，推荐使用Western一抗稀释液(P0023)。
抗原修复过程中应防止缓冲液过度蒸发，切勿干片。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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