碧云天生物技术-Polybrene (Hexadimethrine Bromide)(聚凝胺)(C0351-50mg)

试剂> 细胞相关> 基因表达调控> 病毒感染

Polybrene (Hexadimethrine Bromide)

一键复制产品信息

产品编号: C0351-50mg

产品包装:50mg

选择包装

10mg/ml×1ml 50mg

数

量

说明书下载

价格: ¥ 58.00

加入购物车

				产品简介
			
				使用说明
			
				产品文件
			
				相关产品
			
				相关论文
			
				产品问答

产品编号	产品名称	产品包装	产品价格
C0351-1ml	Polybrene (Hexadimethrine Bromide)	10mg/ml×1ml	53.00元
C0351-50mg	Polybrene (Hexadimethrine Bromide)	50mg	58.00元

Polybrene (聚凝胺)，也称Hexadimethrine Bromide (海美溴铵)，是一种聚溴化季铵阳离子，可显著提高慢病毒(lentivirus)、腺病毒(adenovirus)等病毒对细胞的感染效率。本产品为进口分装。

Polybrene的化学名称为1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide，分子式为(C₁₃H₃₀Br₂N₂)_n，CAS号为28728-55-4，纯度≥94% (titration)。

Polybrene可显著提高慢病毒、腺病毒等病毒对某些细胞的感染效率，其原理可能是通过中和细胞表面唾液酸与病毒颗粒之间的静电排斥从而促进吸附作用。另外，Polybrene也可用于哺乳动物细胞的转染、增强脂质体的转染效率。

带正电荷的Polybrene也是一种抗肝素剂(肝素拮抗剂)，可中和红细胞表面负电荷而常用于制备非特异性凝集的红细胞；其抗凝特性也为体外测定肝素活性提供了一种准确、快速、简单的方法。

Polybrene在蛋白测序中有一定的作用：少量的Polybrene在自动测序分析中可明显增加多肽的降解；加入Polybrene能提高PVDF膜的亲水性，降低测序过程中多肽的机械损伤。

Polybrene也可降低某些酶原的自发激活而用于酶动力学测定。

本产品为进口分装，其中10mg/ml包装产品为超纯水配制并用0.22μm滤膜除菌处理。50mg包装为粉末装。

包装清单：

产品编号	产品名称	包装
C0351-1ml	Polybrene (Hexadimethrine Bromide)	10mg/ml×1ml
C0351-50mg	Polybrene (Hexadimethrine Bromide)	50mg
—	说明书	1份

保存条件：

4℃保存，至少一年有效；-20℃保存，至少二年有效。50mg粉末装须密闭干燥保存。

注意事项：

为避免反复冻融，收到本产品或本产品配制成溶液后建议适当分装后-20℃保存。

因为Polybrene对某些细胞可能有一定的毒性，而且Polybrene长时间作用(大于12小时)也可能对某些细胞产生毒性，所以初次使用Polybrene处理细胞，建议先做毒性测试。

本产品可能对人体有一定的毒害作用，请注意适当防护，以避免直接接触人体或吸入体内。

本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。

为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1.推荐工作浓度：
Polybrene最佳终浓度因不同细胞株而异，通常范围在2~10μg/ml，最常用的浓度为5~8μg/ml。可以通过查阅相关文献或者预实验来摸索。
2.使用方法：
a.Day1：按实验需要将细胞铺板(比如12孔板)。细胞数以第2天密度约50%为宜。37℃培养过夜。
b.Day2：病毒感染前，从-80℃冰箱取出病毒后冰浴融化，参考相关文献或者根据预实验得到的MOI值用新鲜完全培养基将病毒稀释成所需浓度，并加入适量Polybrene，轻轻混匀。
c.吸除细胞原有培养基，将病毒液+培养基+Polybrene加入细胞中，轻轻摇匀。37℃继续培养。
d.Day4：吸除含病毒的培养基，换为新鲜的培养基。此时可以加入相应抗生素进行筛选。
e.Day5-6：根据需要收集细胞检测目的蛋白的表达。
相关产品：

产品编号	产品名称	包装
C0351-1ml	Polybrene (Hexadimethrine Bromide)	10mg/ml×1ml
C0351-50mg	Polybrene (Hexadimethrine Bromide)	50mg
ST551-10mg	Puromycin Dihydrochloride (嘌呤霉素)	10mg/ml×1ml
ST551-50mg	Puromycin Dihydrochloride (嘌呤霉素)	10mg/ml×5ml
ST551-250mg	Puromycin Dihydrochloride (嘌呤霉素)	250mg

物质安全数据单

输入右侧验证码点击下载：

下载

质检证书

输入批号搜寻COA

搜索

相关产品请点击如下按钮：

病毒感染

病毒构建

使用本产品的文献：

1. Bing Yao, Sha Zhu, Xiyi Wei, Ming-Kun Chen, Yangkun Feng, Zhimin Li, Xinyu Xu, Yuwei Zhang, Yang Wang, Jingwan Zhou, Ningyuan Tang, Chengjian Ji, Peng Jiang, Shan-Chao Zhao, Chao Qin, Ninghan Feng

The circSPON2/miR-331-3p axis regulates PRMT5, an epigenetic regulator of CAMK2N1 transcription and prostate cancer progression

Mol Cancer. 2022 May 27;21(1):119. doi: 10.1186/s12943-022-01598-6. (IF 15.302)

2. Ji Wang, Caiguangxi Deng, Ming Liu, Yihao Liu, Liubing Li, Zhangping Huang, Liru Shang, Juan Jiang, Yongyong Li, Ruohui Mo, Hui Zhang, Min Liu, Sui Peng, Haipeng Xiao

A fourth dose of the inactivated SARS-CoV-2 vaccine redistributes humoral immunity to the N-terminal domain

Nat Commun. 2022 Nov 11;13(1):6866. doi: 10.1038/s41467-022-34633-7. (IF 12.121)

3. Wei Wen, Xiangmin Li, Mengge Yin, Haoyuan Wang, Liuxin Qin, Hui Li, Wenqiang Liu, Zekai Zhao, Qiongqiong Zhao, Huanchun Chen, Junjie Hu, Ping Qian

Selective autophagy receptor SQSTM1/ p62 inhibits Seneca Valley virus replication by targeting viral VP1 and VP3

Autophagy. 2021 Nov;17(11):3763-3775. doi: 10.1080/15548627.2021.1897223. (IF 9.77)

4. Jiaren Li, Hanyu Yao, Jin Huang, Chao Li, Yichuan Zhang, Ran Xu, Zhenting Wang, Zhi Long, Jin Tang, Long Wang

METTL3 promotes prostatic hyperplasia by regulating PTEN expression in an m6A-YTHDF2-dependent manner

Cell Death Dis. 2022 Aug 19;13(8):723. doi: 10.1038/s41419-022-05162-4. (IF 6.304)

5. Quanfu Li, Yunkai Chu, Shengze Li, Liping Yu, Huayun Deng, Chunhua Liao, Xiaodong Liao, Chihyu Yang, Min Qi, Jinke Cheng, Guoqiang Chen, Lei Huang

The oncoprotein MUC1 facilitates breast cancer progression by promoting Pink1-dependent mitophagy via ATAD3A destabilization

Cell Death Dis. 2022 Oct 26;13(10):899. doi: 10.1038/s41419-022-05345-z. (IF 6.304)

6. Qiankun Luo, Yanfeng Pan, Qiang Fu, Xu Zhang, Shuai Zhou, Pengfei Yu, Huiyuan Tian, Pan Liu, Song Chen, Hongwei Zhang, Tao Qin

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity

Cell Biol Toxicol. 2022 Feb 10. doi: 10.1007/s10565-022-09695-4. (IF 6.284)

7. Yihao Liu, Qin Zeng, Caiguanxi Deng, Mengyuan Li, Liubing Li, Dayue Liu, Ming Liu, Xinyuan Ruan, Jie Mei, Ruohui Mo, Qian Zhou, Min Liu, Sui Peng, Ji Wang, Hui Zhang, Haipeng Xiao

Robust induction of B cell and T cell responses by a third dose of inactivated SARS-CoV-2 vaccine

Cell Discov. 2022 Feb 1;8(1):10. doi: 10.1038/s41421-022-00373-7. (IF 6.255)

8. Li Yuan, Chunyu Zhang, Jiaying Li, Yuandong Liao, Hua Huang, Yuwen Pan, Qiqiao Du, Yili Chen, Wei Wang, Shuzhong Yao

Profiling and integrated analysis of differentially expressed circRNAs in cervical cancer

Genomics. 2022 Jul;114(4):110418. doi: 10.1016/j.ygeno.2022.110418. (IF 6.205)

9. Yan Zhao, Shuai Jiang, Jian Zhang, Xiao-Lu Guan, Bo-Guang Sun, Li Sun

A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture

Virulence. 2021 Dec;12(1):1362-1376. doi: 10.1080/21505594.2021.1926649. (IF 5.542)

10. Minkai Song, Jiawen Gao, Tao Yan, Enguang Bi, Taixue An, Xiangyu Wang, Weizhou Jiang, Ting Wang, Zishuo Chen, Zhanjun Shi, Chao Zhang, Jun Xiao

Hsa_circ_0000652 Aggravates Inflammation by Activation of Macrophages and Enhancement of OX40/OX40L Interaction in Ankylosing Spondylitis

Front Cell Dev Biol. 2021 Dec 16;9:737599. doi: 10.3389/fcell.2021.737599. (IF 5.201)

11. Zhuo Huang, Ruihuan Yang, Ruyi Li, Yining Zuo, Fan Gu, Miao He, Zhuan Bian

Mesenchymal Mycn participates in odontoblastic lineage commitment by regulating Krüppel-like Factor 4 (Klf4) in mice

Stem Cell Res Ther. 2022 Feb 22;13(1):78. doi: 10.1186/s13287-022-02749-8. (IF 5.116)

12. Wen-Heng Ji, Dan-Dan Li, Dan-Ping Wei, Ai-Qin Gu, Ying Yang, Jing-Pian Peng

Cytochrome P450 26A1 Modulates the Polarization of Uterine Macrophages During the Peri-Implantation Period

Front Immunol. 2021 Oct 12;12:763067. doi: 10.3389/fimmu.2021.763067. (IF 5.085)

13. Yi-Ran Pan, Jing-Yao Song, Bin Fan, Ying Wang, Lin Che, Si-Ming Zhang, Yu-Xin Chang, Chang He, Guang-Yu Li

mTOR may interact with PARP-1 to regulate visible light-induced parthanatos in photoreceptors

Cell Commun Signal. 2020 Feb 17;18(1):27. doi: 10.1186/s12964-019-0498-0. (IF 4.344)

14. Meng Li, Yibin Fan, Yutong Wang, Jinhua Xu, Hui Xu

ZMIZ1 promotes the proliferation and migration of melanocytes in vitiligo

Exp Ther Med. 2020 Aug;20(2):1371-1378. doi: 10.3892/etm.2020.8849. (IF 1.785)

15. Yu Wang, Peihong Zhou, Ping Li, Fengxia Yang, Xue-Qiang Gao

Long non-coding RNA H19 regulates proliferation and doxorubicin resistance in MCF-7 cells by targeting PARP1

Bioengineered. 2020 Dec;11(1):536-546. doi: 10.1080/21655979.2020.1761512. (IF 2.205)

16. Yinan Ji, Xiaoming Liao, Yi Jiang, Wei Wei, Huawei Yang

Aquaporin 1 knockdown inhibits triple-negative breast cancer cell proliferation and invasion in vitro and in vivo

Oncol Lett. 2021 Jun;21(6):437. doi: 10.3892/ol.2021.12698. (IF 2.311)

17. Hongbin Su, Mengdi Qin, Qiang Liu, Bo Jin, Xianjun Shi, Zheng Xiang

Ubiquitin-Like Protein UBD Promotes Cell Proliferation in Colorectal Cancer by Facilitating p53 Degradation

Front Oncol. 2021 Jul 14;11:691347. doi: 10.3389/fonc.2021.691347. (IF 4.848)

18. Wei Wen, Xiangmin Li, Haoyuan Wang, Qiongqiong Zhao, Mengge Yin, Wenqiang Liu, Huanchun Chen, Ping Qian

Seneca Valley Virus 3C Protease Induces Pyroptosis by Directly Cleaving Porcine Gasdermin D

J Immunol. 2021 Jul 1;207(1):189-199. doi: 10.4049/jimmunol.2001030. (IF 4.886)

19. Chengchen Wu, Junyi Zhao, Qiuping Wu, Qiulong Tan, Qiong Liu, Shifeng Xiao

Tau N-Terminal Inserts Regulate Tau Liquid-Liquid Phase Separation and Condensates Maturation in a Neuronal Cell Model

Int J Mol Sci. 2021 Sep 8;22(18):9728. doi: 10.3390/ijms22189728. (IF 4.556)

20. Sheng Li, Leilei Zhang, Guoan Zhang, Guoqiang Shangguan, Xitan Hou, Wanglin Duan, Yan Xi, Nan Xu, Bowen Zhang, Junli Dong, Yequan Wang, Wen Cui, Su Chen

A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation

iScience. 2021 Oct 16;24(11):103296. doi: 10.1016/j.isci.2021.103296.

21. Ling Xie, Jun Yi, Yongjia Song, Mengyao Zhao, Liqiang Fan, Liming Zhao

Suppression of GOLM1 by EGCG through HGF/HGFR/AKT/GSK-3β/β-catenin/c-Myc signaling pathway inhibits cell migration of MDA-MB-231

Food Chem Toxicol. 2021 Nov;157:112574. doi: 10.1016/j.fct.2021.112574. (IF 4.679)

22. Yanhong Wang, Na Li, Yanping Zheng, Anqing Wang, Chunlei Yu, Zhenbo Song, Shuyue Wang, Ying Sun, Lihua Zheng, Guannan Wang, Lei Liu, Jingwen Yi, Yanxin Huang, Muqing Zhang, Yongli Bao, Luguo Sun

KIAA1217 Promotes Epithelial-Mesenchymal Transition and Hepatocellular Carcinoma Metastasis by Interacting with and Activating STAT3

Int J Mol Sci. 2021 Dec 22;23(1):104. doi: 10.3390/ijms23010104. (IF 4.556)

23. Lin Gu, Hailun Zheng, Rui Zhao, Xiaojing Zhang, Qizhi Wang

Diosgenin inhibits the proliferation of gastric cancer cells via inducing mesoderm posterior 1 down-regulation-mediated alternative reading frame expression

Hum Exp Toxicol. 2021 Dec;40(12_suppl):S632-S645. doi: 10.1177/09603271211053292. (IF 2.067)

24. Mo Zhu, Baofei Jiang, Hao Zuo, Xiaopeng Wang, Hengfa Ge, Ziming Huang

LIM-Domain-Binding Protein 1 Mediates Cell Proliferation and Drug Resistance in Colorectal Cancer

Front Surg. 2022 Jan 6;8:790380. doi: 10.3389/fsurg.2021.790380.

25. Tao Liu, Hui Wang, Zhibin Fu, Zheng Wang, Jie Wang, Xinxin Gan, Anbang Wang, Linhui Wang

Methyltransferase-like 14 suppresses growth and metastasis of renal cell carcinoma by decreasing long noncoding RNA NEAT1

Cancer Sci. 2022 Feb;113(2):446-458. doi: 10.1111/cas.15212. (IF 4.966)

26. Xue Li, Yankun Li, Fengxia Li, Qi Chen, Zhonghua Zhao, Xueguang Liu, Nong Zhang, Hui Li

NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy

Int J Mol Sci. 2022 Mar 22;23(7):3458. doi: 10.3390/ijms23073458. (IF 4.556)

27. Huaping Chen, Siyuan Chen, Chen Chen, Aifeng Li, Zhixiao Wei

Leucine zipper downregulated in cancer 1 may serve as a favorable prognostic biomarker by influencing proliferation, colony formation, cell cycle, apoptosis, and migration ability in hepatocellular ca

Front Genet. 2022 Jul 25:13:900951. doi: 10.3389/fgene.2022.900951. (IF 3.258)

28. Fangfang Hu, Yan Ren, Zunyun Wang, Hui Zhou, Yumei Luo, Minghua Wang, Faqing Tian, Jian Zheng, Juan Du, Gang Pang

Bioinformatics analysis of KLF2 as a potential prognostic factor in ccRCC and association with epithelial-mesenchymal transition

Exp Ther Med. 2022 Jul 5;24(3):561. doi: 10.3892/etm.2022.11498. (IF 1.785)

29. Guozheng Cao, Guizhong Zhang, Maoxuan Liu, Junchen Liu, Qi Wang, Lifang Zhu, Xiaochun Wan

GPC3-targeted CAR-T cells secreting B7H3-targeted BiTE exhibit potent cytotoxicity activity against hepatocellular carcinoma cell in the in vitro assay

Biochem Biophys Rep. 2022 Aug 13:31:101324. doi: 10.1016/j.bbrep.2022.101324.

30. Lin Cheng, Chengsheng Xu, Yue Chen, Jian Li, Jing Wang, Jiewen Xiao, Mingjia Ma, Xin Feng, Xiang Wei, Jing Fang

DEC1 represses cardiomyocyte hypertrophy by recruiting PRP19 as an E3 ligase to promote ubiquitination-proteasome-mediated degradation of GATA4

J Mol Cell Cardiol. 2022 Aug:169:96-110. doi: 10.1016/j.yjmcc.2022.05.005. (IF 4.133)

31. Wei Wen, Zixuan Zheng, Haoyuan Wang, Qiongqiong Zhao, Mengge Yin, Huanchun Chen, Xiangmin Li, Ping Qian

Seneca Valley Virus Induces DHX30 Cleavage to Antagonize Its Antiviral Effects

J Virol. 2022 Sep 14;96(17):e0112122. doi: 10.1128/jvi.01121-22. (IF 4.501)

32. Ting Li, Yao Wu, Wei-Cheng Chen, Xing Xue, Mei-Jiao Suo, Ping Li, Wei Sheng, Guo-Ying Huang

Functional analysis of HECA variants identified in congenital heart disease in the Chinese population

J Clin Lab Anal. 2022 Sep;36(9):e24649. doi: 10.1002/jcla.24649. (IF 1.54)

33. Chunsheng Hu, Anting Zhou, Xin Hu, Yu Xiang, Mengjun Huang, Jiuhong Huang, Donglin Yang, Yan Tang

LMNA Reduced Acquired Resistance to Erlotinib in NSCLC by Reversing the Epithelial-Mesenchymal Transition via the FGFR/MAPK/c-fos Signaling Pathway

Int J Mol Sci. 2022 Oct 31;23(21):13237. doi: 10.3390/ijms232113237. (IF 4.556)

34. Mingzhe Li, Jiaxin Zhou, Zhengkui Zhang, Jisong Li, Feng Wang, Ling Ma, Xiaodong Tian, Zebin Mao, Yinmo Yang

Exosomal miR-485-3p derived from pancreatic ductal epithelial cells inhibits pancreatic cancer metastasis through targeting PAK1

Chin Med J (Engl). 2022 Oct 5;135(19):2326-2337. doi: 10.1097/CM9.0000000000002154.

35. Qun Liu, Jingyu Tan, Zhenguo Zhao, Ruijun Li, Luyu Zheng, Xiangyu Chen, Lina Li, Xichen Dong, Tao Wen, Jian Liu

Combined Usage of MDK Inhibitor Augments Interferon-γ Anti-Tumor Activity in the SKOV3 Human Ovarian Cancer Cell Line

Biomedicines. 2022 Dec 21;11(1):8. doi: 10.3390/biomedicines11010008.

36. Ling Wang, Jin Wang, Ling Chen

TIMP1 represses sorafenib-triggered ferroptosis in colorectal cancer cells by activating the PI3K/Akt signaling pathway

Immunopharmacol Immunotoxicol. 2023 Dec;45(4):419-425. doi: 10.1080/08923973.2022.2160731.

浏览此产品的顾客也同时浏览：

C0351-1ml

10mg/ml×1ml

Polybrene (Hexadimethrine Bromide)

ST551-10mg

10mg/ml×1ml

Puromycin Dihydrochloride (嘌呤霉素)

ST1380-500mg

500mg

Polybrene (≥94%, Reagent grade)

ST1380-10g

10g

Polybrene (≥94%, Reagent grade)

ST1380-2g

Polybrene (≥94%, Reagent grade)

C2901M

可用于600ml

通用型病毒浓缩试剂盒

用户名：
密码：
校验码：
	后续自动登录

	找回密码注册

新冠相关产品

试剂

核酸相关

多肽与蛋白

细胞相关

信号转导

抗体

抑制剂激活剂等

常用试剂

耗材

仪器

技术服务