碧云天生物技术-免疫染色通透液(Triton X-100)(P0096-500ml)

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免疫染色通透液(Triton X-100)

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产品编号: P0096-500ml

产品包装:500ml

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产品编号	产品名称	产品包装	产品价格
P0096-100ml	免疫染色通透液(Triton X-100)	100ml	40.00元
P0096-500ml	免疫染色通透液(Triton X-100)	500ml	143.00元

免疫染色通透液(Triton X-100) (Immunostaining Permeabilization Buffer with Triton X-100, 也称Immunostaining
Permeabilization Solution with Triton X-100)，可以用于免疫染色等多种原位检测时细胞样品、冰冻或石蜡切片的通透处理，有助于暴露抗原、核酸等作用靶点，使抗体、探针或标记物等更容易进入细胞内，从而确保染色等的检测效果。本产品为即用型工作液，可以直接使用，无需稀释。
本产品通透能力较强，推荐用于各种常规的免疫荧光、免疫组化、免疫细胞化学、流式检测等中细胞的通透。对于通透效果要求不高的情况，也可以使用碧云天的免疫染色洗涤液(P0106, P0106C, P0106L)，对于需要检测细胞膜蛋白的情况推荐使用免疫染色通透液(Saponin) (P0095)。对于通透要求比较高的情况，可以使用免疫染色强力通透液(P0097)。对于凝集素(lectin)的检测推荐使用本产品或免疫染色强力通透液(P0097)。
通透液通常可以通过使用有机溶剂例如甲醇、丙酮等，或者使用去垢剂例如Triton X-100、Saponin等来实现对于细胞膜的通透。甲醇或丙酮等有机溶剂，一方面可以溶解细胞膜和核膜从而充分暴露细胞浆和细胞核中的目的蛋白等，另一方面也可以使细胞内的蛋白变性起到固定的作用。甲醇或丙酮等有机试剂操作比较简单，一步处理可以同时实现固定和通透，缺点是膜蛋白也会被溶解掉并且有些蛋白变性后不利于后续的检测，因此有机溶剂使用相对较少，仅用于一些要求比较粗糙的检测。Triton X-100是常用的通透试剂，可以通透细胞膜和核膜，作用的原理也是非特异性地溶解细胞膜，因此其缺点是不利于膜蛋白的检测，但当使用多聚甲醛进行交联和固定后，相当一部分膜蛋白会被交联固定，从而不会被Triton X-100溶解，因此后续仍然能检测到。
Saponin可以特异性地溶解细胞膜中的胆固醇，从而实现在细胞膜上选择性地打孔，其优点是适合于细胞膜蛋白的检测，特别适合通过流式细胞仪检测细胞膜上的标志性蛋白，缺点是对于一些胆固醇含量低的细胞通透效果差，通透效果弱于Triton X-100和有机溶剂，并且不能通透胆固醇含量很低的核膜和线粒体膜。对于凝集素(lectin)的检测，含Triton X-100等非特异性去垢剂的通透液的效果显著优于主要含Saponin的通透液。
本免疫染色通透液(Triton X-100)中含有Triton X-100等去垢剂，配制在PBS中。经本免疫染色通透液(Triton X-100)处理的样品，对于细胞浆或细胞核内目的蛋白的免疫染色检测结果表明，染色效果与常规的通透液相比基本一致或略有增强。
按照每个样品需要0.1或1毫升免疫染色通透液(Triton X-100)计算，一个100ml包装的本产品可以通透1000个或100个样品，一个500ml包装的本产品可以通透5000个或500个样品。
包装清单：

产品编号	产品名称	包装
P0096-100ml	免疫染色通透液(Triton X-100)	100ml
P0096-500ml	免疫染色通透液(Triton X-100)	500ml
—	说明书	1份

保存条件：
4℃保存，一年有效。-20℃可以长期保存，室温保存一个月内有效。
注意事项：
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 对于切片，在固定、洗涤结束后，每样滴50-100μl免疫染色通透液(Triton X-100)，也可以在染色缸中完全浸没进行通透。对于细胞样品，在固定、洗涤结束后，按照六孔板每孔加入1毫升免疫染色通透液(Triton X-100)，其它多孔板参考该比例加入。对于其它样品，加入免疫染色通透液(Triton X-100)的量以充分盖住样品为准。
2. 通常在免疫染色通透液(Triton X-100)中室温孵育5-10分钟，即可完成通透。对于较难通透的样品或者要求通透特别充分的情况，可以在免疫染色通透液(Triton X-100)中室温孵育10-30分钟。

相关产品：

产品编号	产品名称	包装
P0095-100ml	免疫染色通透液(Saponin)	100ml
P0095-500ml	免疫染色通透液(Saponin)	500ml
P0096-100ml	免疫染色通透液(Triton X-100)	100ml
P0096-500ml	免疫染色通透液(Triton X-100)	500ml
P0097-100ml	免疫染色强力通透液	100ml
P0097-500ml	免疫染色强力通透液	500ml
P0098	免疫染色固定液	100ml
P0102	免疫染色封闭液	100ml
P0106	免疫染色洗涤液	250ml
P0106C	免疫染色洗涤液(10X)	100ml
P0106L	免疫染色洗涤液(10X)	10×100ml
P0260	QuickBlock™免疫染色封闭液	100ml

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